Thyroid Function Changes in a Rat Model of Vitamin D Deficiency and Effect of Vitamin D and Metformin Treatment

Abulfadle, Khaled Abdelfattah; Abozaid, Eman R.; Rahman, Maha M. Abdul

doi:10.21608/mjcu.2020.94014

Cited by 2 publications

(1 citation statement)

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“…Rats were divided into two groups; control rats (n = 8) that received daily oral administration of tap water (2 ml/kg body weight) till the end of the experiment, and rat model of hypothyroidism (n = 32) induced by daily oral administration of PTU (15 mg/kg body weight) for 45 days [23]. Starting from the 46th day the animals of the rat model of hypothyroidism were divided into four groups: rat model of hypothyroidism (n = 8) that received PTU (15 mg/kg body weight) for 30 days, rat model of hypothyroidism (n = 8) treated daily with MET orally (300 mg/kg body weight) [24] one hour after the daily oral administration of PTU (15 mg/kg body weight) for 30 days, rat model of hypothyroidism (n = 8) treated daily with LA orally (100 mg/kg body weight) [25] for 30 days one hour after the daily oral administration of PTU (15 mg/kg body weight) and rat model of hypothyroidism (n = 8) co-treated daily with MET and LA orally one hour between each treatment for 30 days. At the end of the experiment, blood samples were collected from the retro-orbital venous plexus of the animals in clean heparinized capillary tubes and then centrifuged at 3000 rpm for 15 minutes at 4°C to separate sera.…”

Section: Experimental Designmentioning

confidence: 99%

Cardioprotective effects of metformin and alpha lipoic acid against myocardial complications induced by hypothyroidism in rats

Khadawy,

Hosny,

Abou-Seif

et al. 2023

Preprint

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The therapeutic effects of alpha lipoic acid (LA) and metformin (MET) against the myocardial complications associated with hypothyroidism were assessed in the current study. Rats were divided into three groups: control, hypothyroidism model induced by propylthiouracil (PTU), hypothyroidism model treated with LA and/or MET. At the end of the experiment, the thyroid hormones (T3, T4 and TSH) were measured in the serum. Lipid peroxidation (MDA), nitric oxide (NO), reduced glutathione (GSH), NrF2, BCL2, NF-κB, serotonin (5-HT), norepinephrine (NE) and dopamine (DA), acetylcholinesterase (AchE), monoamine oxidase (MAO) and Na+,K+,ATPase were measured in the cardiac tissue. The histopathological changes were also examined. PTU significantly decreased T3 and T4 and significantly increased TSH. A significant increase in MDA, GSH, NrF2, BCL2, NF-κB, 5-HT, NE, DA, AchE, MAO and Na+,K+,ATPase and a significant decrease in NO were observed in the cardiac tissue of hypothyroidism model. This was associated with histopathological changes. LA alone or with MET restored T3 and TSH and improved almost all the biochemical changes except the decreased NO and the increased DA in the cardiac tissue. MET restored T3, T4 and TSH and the biochemical changes induced in the cardiac tissue. MET ameliorated the histopathological changes that were still observed with LA alone or in combination with MET. The present findings indicate that MET had cardioprotective effect against hypothyroidism and its myocardial complications. This effect was less prominent with LA alone or in combination with MET. No synergistic effect was observed between LA and MET.

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Section: Experimental Designmentioning

confidence: 99%