Thyroid hormone and retinoic acid induce the synthesis of insulin-like growth factor-binding protein-4 in prepubertal pig sertoli cells

G, Bardi; Bottazzi, C; Demori, Ilaria; Palmero, S.

doi:10.1530/eje.0.1410637

Cited by 14 publications

(7 citation statements)

References 52 publications

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“…In this work, we have shown that hepatic IGFBP-4 expression is stimulated simultaneously by RA and T 3 , in agreement with data from other experimental models [38,39]. Both agents induce an increase in the IGFBP-4 mRNA content after several hours of incubation, and the effects are similar in magnitude.…”

Section: Discussionsupporting

confidence: 90%

Retinoic Acid Increases Insulin-like Growth Factor-binding Protein-4 Expression in Cultured Rat Hepatocytes

2004

Horm Metab Res

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Hepatic insulin-like growth factor binding protein (IGFBP) expression is controlled by diverse factors including thyroid hormone, which enhances IGFBP-4 production in hepatocytes. In the present work, we have investigated whether hepatic IGFBP-4 expression is regulated by retinoic acid (RA), which acts via nuclear receptors belonging to the steroid/thyroid hormone receptor superfamily. Primary cultures of adult rat hepatocytes were incubated with two natural stereoisomers of RA, all-trans RA and 9-cis RA (atRA and 9cRA), and with the synthetic RA receptor (RAR)-selective agonist TTNPB. IGFBP-4 mRNA abundance was measured by Northern blot and protein production was evaluated by Ligand blot on hepatocyte-conditioned culture media. Our results indicate that atRA, 9cRA, and TTNPB increase IGFBP-4 expression by cultured hepatocytes, both at the mRNA and protein level. The RARs play a definite role in this regulation, which is independent from ongoing protein synthesis but dependent on active transcription. AtRA and thyroid hormone act synergistically in increasing hepatic IGFBP-4 expression. Our data establish a role for hormonal factors such as thyronines and retinoids in regulating the hepatic IGF system directly at the IGFBP-4 level.

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Section: Discussionsupporting

confidence: 90%

Retinoic Acid Increases Insulin-like Growth Factor-binding Protein-4 Expression in Cultured Rat Hepatocytes

2004

Horm Metab Res

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“…Early observations suggest that T3 regulates some of these paracrine factors. T3 stimulates Sertoli cell IGF-I secretion and IGFBP-4 mRNA expression [12,82]. It also stimulates serum immunoreactive inhibin-a levels and mRNA expression [8,100].…”

Section: Thyroid Hormones and Testicular Paracrine Factorsmentioning

confidence: 99%

Thyroid Hormones: Their Role in Testicular Steroidogenesis

Maran

2003

Archives of Andrology

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“…Only half of the 20 genes were known to be expressed in Sertoli cells, i.e. c-jun, cyclin D2, GRP78 (Day and Lee 1989), IRF1 (Kanzaki and Morris 1998), proliferating cell nuclear antigen (PCNA; Schlatt and Weinbauer 1994), inhibin b A subunit (Kaipia et al 1992), IGFBP-3 (Smith et al 1990), IGFBP-4 ( Bardi et al 1999), cytokeratin-18 (Stosiek et al 1990) and Timp-2 (Grima et al 1996).…”

Section: Resultsmentioning

confidence: 99%

Gene expression profiling of mouse Sertoli cell lines

Strothmann¹,

Simoni²,

Mathur

et al. 2004

Cell and Tissue Research

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The proliferation and differentiation of Sertoli cells is regulated by follicle-stimulating hormone (FSH). The molecular events following FSH stimulation are only partially known. To investigate FSH action in Sertoli cells, we established two novel FSH-responsive mouse Sertoli-cell-derived lines expressing human wild-type (WT) FSH receptor (FSHR) or overexpressing mutated (Asp567Gly) constitutively active FSHR (MUT). Gene expression profiling with commercially available cDNA arrays, including 588 mouse genes, revealed 146 genes expressed in both cell lines. Compared with the expression pattern of WT cells, 20 genes were identified as being either up- or down-regulated (>two-fold) in the MUT cells. We observed a strong differential expression of factors involved in cellular proliferation, e.g. cyclin D2 (repressed to nearly undetectable levels), proliferating cell nuclear antigen (2.5-fold repression) and Eps-8 (six-fold repression), and in genes involved in cellular differentiation, e.g. cytokeratin-18 (13-fold induction). The cDNA array results for six representative genes were confirmed by Northern blotting, which also included the parental SK-11 cell line devoid of FSHR expression. We found no further acute FSH- or forskolin-induced change in expression levels after 3-h stimulations, suggesting that the observed differences between the two cell lines is a consequence of mild, chronically increased, cAMP production in MUT cells. These results provide a platform for the further investigation of selected candidate genes in primary cultures and/or in vivo.

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Thyroid hormone and retinoic acid induce the synthesis of insulin-like growth factor-binding protein-4 in prepubertal pig sertoli cells

Cited by 14 publications

References 52 publications

Retinoic Acid Increases Insulin-like Growth Factor-binding Protein-4 Expression in Cultured Rat Hepatocytes

Retinoic Acid Increases Insulin-like Growth Factor-binding Protein-4 Expression in Cultured Rat Hepatocytes

Thyroid Hormones: Their Role in Testicular Steroidogenesis

Gene expression profiling of mouse Sertoli cell lines

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