Thyroid Hormone Inhibits Aromatase Activity in Porcine Thecal Cells Cultured Alone and in Coculture with Granulosa Cells

Gregoraszczuk, Ewa Ł.; Słomczyńska, Maria; Wilk, Renata

doi:10.1089/thy.1998.8.1157

Cited by 30 publications

(12 citation statements)

References 14 publications

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“…In an in vitro system, T 3 caused about a twofold increase of protein synthesis in rat granulosa cells compared to cells without T 3 (Bandyopadhyay et al, 1996). In vitro T 3 assisted FSH and LH to enhance steroid biosynthesis in porcine (Chan and Tan, 1986;Maruo et al, 1987;Gregoraszczuk et al, 1998) and human (Goldman et al, 1993;Wakim et al, 1995a, b) granulosa cells. A recent study has reported that T 3 mediated stimulation of P 4 release from human luteal cells is not direct, but is mediated through a putative protein factor (Datta et al, 1998).…”

Section: Yroid Hormonal Regulation Of Ovarian Endocrine Functionmentioning

confidence: 99%

Clinical endocrinology of thyroid gland function in ruminants

Huszenicza¹,

Kulcsár²,

Rudas³

2002

Vet. Med.

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After briefly introducing the basec steps in production and metabolism of thyroid hormones, the author gives an overview of nutritional, metabolic and disease related factors and endocrine interactions influencing thyroid gland function in ruminants, particularly in the postpartum dairy cow. Involvement of thyroid hormonal regulation of seasonal patterns of reproduction as well as ovarian endocrine function are discussed.Keywords: thyroxin; triiodothyronine; ovary; metabolism; ruminants Abbreviations: 5'D = 5'-deiodinase (syn.: outer-ring deiodinase); 5D = 5-deiodinase (syn.: inner-ring deiodinase); BHB = βOH-butyrate; E 2 = 17β-estradiol; FSH = follicle stimulating hormone; GH = growth hormone; GnRH = gonadotropin releasing hormone; hCG = human chorionic gonadotropin; IGF-I = insulin-like growth factor-I (syn.: somatomedin C); IL = interleukin; LH = luteinizing hormone; mRNA = messenger ribonucleic acid; NEB = negative energy balance; NEFA = non-esterified fatty acids; P 4 = progesterone; PVN = paraventricular nucleus of the hypothalamus; rT 3 = 3,3',5'-triiodothyronine (syn.: reverse-triiodothyronine); T 3 = 3,3',5-triiodothyronine; T 4 = thyroxine; TNFα = tumor necrosis factor-α; TRH = thyrotropin-releasing hormone; TSH = thyroid-stimulating hormone (syn.: thyrotropin)

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Section: Yroid Hormonal Regulation Of Ovarian Endocrine Functionmentioning

confidence: 99%

Clinical endocrinology of thyroid gland function in ruminants

Huszenicza¹,

Kulcsár²,

Rudas³

2002

Vet. Med.

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“…In porcine GCs, T 4 stimulates FSH-induced estradiol and progesterone secretion (26). Wakim et al (25) additionally demonstrated that T 4 augments steroidogenesis in human GCs.…”

Section: Discussionmentioning

confidence: 98%

Effect of Thyroid Hormone on CYP19 Expression in Ovarian Granulosa Cells From Gonadotropin-Treated Immature Rats

et al. 2004

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Abstract. The present study was designed to determine the mechanisms by which serum estradiol levels are enhanced during gonadotropin-induced ovarian development in hypothyroid immature rats. Thyroidectomized (Tx)-immature rats receiving thyroidectomy on day 22 were treated with 8 IU equine chorionic gonadotropin (eCG) at 26 days of age to induce follicular development in ovaries. Ovaries or ovarian granulosa cells were collected on the first proestrus (day 28). Enhanced expression of aromatase (CYP19) mRNA induced by eCG treatment was inhibited by injection of thyroxine (T 4 : 5 m g´6 times) in Tx rats. Ovarian granulosa cells harvested from Tx rats were cultured for 24 h in chemically defined serum-free medium containing gonadotropin in the presence of 3,5,3'-triidothyronine (T 3 ). Addition of low doses of T 3 (10 -8 , 10 -7 M) to the medium significantly decreased 17b-estradiol (E 2 ) levels, whereas high doses of T 3 (10 -6 , 10 -5 M) did not inhibit the levels. Similar effects of T 3 on CYP19 mRNA expression were observed. These results indicate that augmentation of E 2 production in hypothyroid animals may be mediated, in part, via the stimulation of CYP19 mRNA expression in granulosa cells, which is suppressed by thyroid hormone.

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“…At the same time, these hormones significantly inhibit apoptosis during in vitro cultivation of porcine granular layer cells. They also participate in the regulation of steroidogenesis by inducing or inhibiting the effect on steroidogenic enzymes [39] [40].…”

Section: Factors Influencing Follicular Steroidogenesismentioning

confidence: 99%

Characteristic of factors influencing the proper course of folliculogenesis in mammals

Rybska

Knap

Jankowski

et al. 2018

Medical Journal of Cell Biology

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Folliculogenesis is the process of ovarian follicle formation,, taking presence during foetal period. During the follicular development, oogoniums undergo meiosis and oocytes are formed. In the ovaries of new born sows, primary and secondary follicles are present and, 90 days after birth, tertiary follicles appear. During development in the ovarian follicles growth of granulosa cells and differentiation of the thecal cells can be observed. A cavity filled with follicular fluid appears. Granulosa cells are divided into: mural cells and corona radiata, which together with the oocyte form the cumulus oophorus. Corona radiata cells, mural layers and oolemma contact each other by a network of gap junctions. Secreted from the pituitary gland, FSH and LH gonadotropin hormones act on receptors located in granular and follicular cells. In the postnatal life tertiary follicles and Graafian follicles are formed. When the follicle reaches a diameter of 1 mm, further growth depends on the secretion of gonadotropins. Mature ovarian follicles produce: progestins, androgens and oestrogens. The growth, differentiation and steroidogenic activity of ovarian follicles, in addition to FSH and LH, is also affected by prolactin, oxytocin, steroid and protein hormones, numerous proteins from the cytokine and interleukin family, metabolic hormones like insulin, glucocorticoids, leptin, thyroid hormones and growth hormones. Despite numerous studies, many processes related to folliculogenesis have not been discovered Learning the mechanisms regulating reproductive processes would allow to easily distinguish pathological processes and discover more and more genes and mechanisms of their expression in cells that build ovarian follicles. Running title: regulation of folliculogenesis

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Thyroid Hormone Inhibits Aromatase Activity in Porcine Thecal Cells Cultured Alone and in Coculture with Granulosa Cells

Cited by 30 publications

References 14 publications

Clinical endocrinology of thyroid gland function in ruminants

Clinical endocrinology of thyroid gland function in ruminants

Effect of Thyroid Hormone on CYP19 Expression in Ovarian Granulosa Cells From Gonadotropin-Treated Immature Rats

Characteristic of factors influencing the proper course of folliculogenesis in mammals

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