Thyroid hormone regulates protein expression in C6 glioma cells

Trentin, Andréa Gonçalves; Alvarez-Silva, Márcio

doi:10.1590/s0100-879x1998001000008

Cited by 11 publications

(7 citation statements)

References 20 publications

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“…The rat glioblastoma C6 cell line was a gift from Dr. Vivaldo Moura Neto (Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil) and cultured as previously described (Trentin and Alvarez-Silva, 1998). Cells were grown in Dulbecco's modified Eagle's medium (DMEM, Sigma, St. Louis) supplemented with 5% fetal calf serum (FCS, Fazenda Pigue, Brazil), 100 mg/ml streptomycin and 100 U/ml penicillin (all from Sigma) in humidified incubator at 37 • Ci na5 %C O 2 , 95% air atmosphere (standard culture conditions).…”

Section: Cell Culturementioning

confidence: 99%

Undersulfation of proteoglycans and proteins alter C6 glioma cells proliferation, adhesion and extracellular matrix organization

Aguiar

Garcez

Alvarez-Silva

et al. 2002

Intl J of Devlp Neuroscience

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Proteoglycans are considered to be important molecule in cell-microenvironment interactions. They are overexpressed in neoplastic cells modifying their growth and migration in hosts. In this work we verified that undersulfation of proteoglycans and other sulfated molecules, induced by sodium chlorate treatment, inhibited C6 glioma cells proliferation in a dose-dependent way. This effect was restored by the addition of exogenous heparin. We could not detect significant cell mortality in our culture condition. The treatment also impaired in a dose-dependent manner, C6 cell adhesion to extracellular matrix (ECM) proteins (collagen IV, laminin and fibronectin). In addition, sodium chlorate treatment altered C6 glioma cell morphology, from the fibroblast-like to a more rounded one. This effect was accompanied by increased synthesis of fibronectin and alterations in its extracellular network organization. However, we could not observe modifications on laminin organization and synthesis. The results suggest an important connection between sulfation degree with important tumor functions, such as proliferation and adhesion. We suggest that proteoglycans may modulate the glioma microenvironment network during tumor cell progression and invasion.

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Section: Cell Culturementioning

confidence: 99%

Undersulfation of proteoglycans and proteins alter C6 glioma cells proliferation, adhesion and extracellular matrix organization

Aguiar

Garcez

Alvarez-Silva

et al. 2002

Intl J of Devlp Neuroscience

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“…Established permanent cell lines offer an excellent opportunity to study several aspects of glioma pathology, which may be pertinent to diagnostic and therapeutic issues (Kruse et al, 1998;Nederman, 1984;Laerum et al, 1985;Linksey and Gilbert, 1995;Trentin and Alvarez-Silva, 1998;Michailowsky et al, 2003). NG97 cell line was established and partially characterized in 2001 by Grippo and co-workers from a temporal grade III astrocytoma (WHO, 2000;Cavenee et al, 2000) in a 66-yearold male.…”

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confidence: 99%

Immunophenotypic and Ultrastructural Validation of a New Human Glioblastoma Cell Line

et al. 2005

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1. A human glioma cell line, NG97, was established by Grippo et al. in 2001 from tissue obtained from a grade III astrocytoma (WHO, 2000). In this first study, the cell line grew as two morphologically distinct subpopulations: dendritic/spindle cells and small round cells. The injection of NG97 cells into nude mice induced an aggressive tumor characterized by: severe cytological atypia, vascular proliferation and pseudopalisading necrosis (glioblastoma multiforme features). 2. The purpose of the present study was to characterize the immunophenotype and ultrastructural aspects of this cell line, using the parental tumor, cultured cells and the xenotransplant, in order to assess its glial nature and possible divergent differentiation. 3. NG97 cells and xenotransplant expressed the main neuroglial markers (GFAP, S-100 protein, NSE and Leu-7) and showed no aberrant expression of other histogenetic markers. GFAP was similarly expressed in the parental tumor and in the cells in culture, but decreased in the xenotransplant. NSE expression was reduced in NG97 cells, but substantially recovered in the xenotransplant. This variability in expression of GFAP and NSE was interpreted as either a phenomenon of dedifferentiation or to microenvironmental selection of specific subclones. S-100 was equally expressed in the three contexts. The xenotransplant's ultrastructural features were those of a highly undifferentiated tumor. No significant immunophenotypic or ultrastructural differences between the two morphologically distinct populations were found. 4. Thus, our data demonstrate that NG97 cells constitute a pure glial-committed cell line, which may prove useful as a malignant glioma model in studies addressing pathophysiological, diagnostic and therapeutic issues.

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“…The C6 cell line originated from a chemically induced rat brain tumor and expresses GFAP and vimentin [31], [32], which is a property of undifferentiated astrocytic cell type [33]. B35 cells were derived from a neonatal rat brain tumor displaying neuronal characteristics: showing membrane excitability, expression of enzymes for neurotransmitters and neuron-specific enolase [34].…”

Section: Discussionmentioning

confidence: 99%

TTF-1 Action on the Transcriptional Regulation of Cyclooxygenase-2 Gene in the Rat Brain

et al. 2011

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We have recently found that thyroid transcription factor-1 (TTF-1), a homeodomain-containing transcription factor, is postnatally expressed in discrete areas of the hypothalamus and closely involved in neuroendocrine functions. We now report that transcription of cyclooxygenase-2 (COX-2), the rate limiting enzyme in prostaglandin biosynthesis, was inhibited by TTF-1. Double immunohistochemistry demonstrated that TTF-1 was expressed in the astrocytes and endothelial cells of blood vessel in the hypothalamus. Promoter assays and electrophoretic mobility shift assays showed that TTF-1 inhibited COX-2 transcription by binding to specific binding domains in the COX-2 promoter. Furthermore, blocking TTF-1 synthesis by intracerebroventricular injection of an antisense oligomer induced an increase of COX-2 synthesis in non-neuronal cells of the rat hypothalamus, and resulted in animals' hyperthermia. These results suggest that TTF-1 is physiologically involved in the control of thermogenesis by regulating COX-2 transcription in the brain.

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Thyroid hormone regulates protein expression in C6 glioma cells

Cited by 11 publications

References 20 publications

Undersulfation of proteoglycans and proteins alter C6 glioma cells proliferation, adhesion and extracellular matrix organization

Undersulfation of proteoglycans and proteins alter C6 glioma cells proliferation, adhesion and extracellular matrix organization

Immunophenotypic and Ultrastructural Validation of a New Human Glioblastoma Cell Line

TTF-1 Action on the Transcriptional Regulation of Cyclooxygenase-2 Gene in the Rat Brain

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