2018
DOI: 10.1126/science.aau6348
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Thyroid hormone signaling specifies cone subtypes in human retinal organoids

Abstract: The mechanisms underlying specification of neuronal subtypes within the human nervous system are largely unknown. The blue (S), green (M), and red (L) cones of the retina enable high-acuity daytime and color vision. To determine the mechanism that controls S versus L/M fates, we studied the differentiation of human retinal organoids. Organoids and retinas have similar distributions, expression profiles, and morphologies of cone subtypes. S cones are specified first, followed by L/M cones, and thyroid hormone s… Show more

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Cited by 220 publications
(192 citation statements)
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“…There was significantly higher number of RHODOPSIN‐positive cells in WT1 organoids differentiated with Method II (Figure B); WT2 organoids differentiated with the same method showed the presence of RECOVERIN‐ and RHODOPSIN‐ positive cells and OPSIN RED/GREEN positive cells could also be observed; however, protein localization to the outer segments was not always apparent. The reported M/L‐ to S‐cone (red/green to blue) ratios range between 5 and 8 to 1 . We observed similar proportions in WT1 and WT2 Method I‐derived organoids (Figure C).…”
Section: Resultssupporting
confidence: 64%
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“…There was significantly higher number of RHODOPSIN‐positive cells in WT1 organoids differentiated with Method II (Figure B); WT2 organoids differentiated with the same method showed the presence of RECOVERIN‐ and RHODOPSIN‐ positive cells and OPSIN RED/GREEN positive cells could also be observed; however, protein localization to the outer segments was not always apparent. The reported M/L‐ to S‐cone (red/green to blue) ratios range between 5 and 8 to 1 . We observed similar proportions in WT1 and WT2 Method I‐derived organoids (Figure C).…”
Section: Resultssupporting
confidence: 64%
“…The development of in vitro retinal models has been driven by a lack of adequate animal models that recapitulate the structure and function of the human retina. Induced pluripotent stem cell (IPSC)‐derived retinal organoids have been shown to have a wide range of applications, including the study of human retinogenesis, disease modeling, drug discovery, and cell therapy . Numerous protocols have been developed for the generation of retinal organoids that follow basic developmental principles of forebrain development and eye formation.…”
Section: Introductionmentioning
confidence: 99%
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“…Interestingly, CCDC136 is located next to the OPN1SW locus in humans and could possibly be co-regulated at the transcriptional level. The thyroid hormone receptor THRB is required for the development of M-cones in mice (Ng et al, 2001) and L/M-cones in humans as determined by a pluripotent stem cell model (Eldred et al, 2018). Notably, there are two known receptor isoforms for THRB (TRb1 and TRb2) and further research to determine the precise roles of THRB isoforms in subtype specification of human cones would be of great interest.…”
Section: Profiling Healthy and Degenerating Human Rod Photoreceptor Smentioning
confidence: 99%
“…These different protocols have been the basis for many others that followed, and they have been replicated using mESCs, hESCs, and iPSCs (mouse and human) ( Table 1) (Osakada et al, 2008;Parameswaran et al, 2010;Tucker et al, 2011a;Tucker et al, 2011b;La Torre et al, 2012;Nakano et al, 2012;Boucherie et al, 2013;Tucker et al, 2013;Decembrini et al, 2014;Riazifar et al, 2014;Zhong et al, 2014;La Torre et al, 2015;Sluch et al, 2015;Tanaka et al, 2015;Volkner et al, 2016;Aparicio et al, 2017;Chao et al, 2017;Teotia et al, 2017;Vergara et al, 2017;Yokoi et al, 2017;Daniszewski et al, 2018;DiStefano et al, 2018;Kobayashi et al, 2018;Langer et al, 2018). Retinal organoids have also proven useful as platforms to study different aspects of development using not only mouse cells, but also human cells Phillips et al, 2014;Ohlemacher et al, 2016;Takata et al, 2017;Eldred, 2018). The latter is especially crucial since human developmental studies are often unfeasible given the limited availability of tissue and the difficulties to perform genetic manipulations.…”
Section: Stem Cell Strategies To Generate Rgcs In Vitromentioning
confidence: 99%