We report an improved measurement of νµ disappearance over a distance of 735 km using the MINOS detectors and the Fermilab Main Injector neutrino beam in a νµ-enhanced configuration. From a total exposure of 2.95 × 10 20 protons on target, of which 42% have not been previously analyzed, we make the most precise measurement of ∆m 2 = [2.62 +0.31 −0.28 (stat.)±0.09(syst.)] ×10 −3 eV 2 and constrain the νµ mixing angle sin 2 (2θ) > 0. 75 (90%CL). These values are in agreement with ∆m 2 and sin 2 (2θ) measured for νµ, removing the tension reported in [1]. The CPT symmetry of the SM requires that ν µ and ν µ have the same masses and mixing parameters. In vacuum, the probability P (ν µ → ν µ ) that a ν µ is detected after a distance L as a ν µ (rather than a ν e or ν τ ) must be equal to the corresponding probability P (ν µ → ν µ ) for antineutrinos. For a ν µ with energy E the probability may be written as(1) where ∆m 2 and sin 2 (2θ) are effective parameters that are functions of the angles parameterizing U PMNS and the differences in the squared masses ∆m [15,16]. Muon antineutrino oscillations are described by an equation which has the same form as Eq. 1 with parameters ∆m 2 and sin 2 (2θ). The extended SM predicts ∆m 2 = ∆m 2 and sin 2 (2θ) = sin 2 (2θ) for vacuum oscillations [17,18]. Observation of P (ν µ → ν µ ) = P (ν µ → ν µ ) would therefore be evidence for physics beyond the SM, such as neutrino interactions in the earth's crust that do not conserve lepton flavor.In this Letter we describe a measurement of P (ν µ → ν µ ) conducted over a baseline L = 735 km using a ν µ -enhanced beam with a peak energy of 3 GeV. The beam was produced by directing 120 GeV/c protons from the Fermilab Main Injector onto a graphite target to produce π/K mesons that decay to produce neutrinos. Two magnetic horns focus the mesons, allowing us to control the energy spectrum and ν/ν content of the beam.The neutrino beam is pointed towards two detectors, referred to as Near and Far. The 980 ton Near Detector (ND) measures the ν µ and ν µ content of the beam as a function of energy at a distance of 1.04 km from the π/K production target. The 5.4 kton Far Detector (FD) is located in the Soudan Underground Laboratory, 734 km from the ND, and remeasures the beam composition. The neutrino detectors are steel-scintillator, tracking-sampling calorimeters optimized to identify and measure the energy of muon neutrinos and antineutrinos and reject backgrounds from neutral current and ν e interactions [19]. The detectors are magnetized with an average field of 1.3 T to distinguish ν µ from ν µ based on the charge of the µ produced in weak interactions.We previously reported ν µ oscillations with an energy dependence consistent with Eq. 1 and ∆m 2 = 2.32 +0.12 −0.08 (stat.+syst.) ×10 −3 eV 2 , sin 2 (2θ) In 2009-2010 we collected 1.71 × 10 20 POT in a ν µ -enhanced beam [20] created by reversing the polarity of the horns. The magnetic fields in the FD and ND were also reversed to focus the µ + created in ν µ interactions. These antineutrino d...