Thyroid Hormones Stimulate Erythropoiesis in Vitro

Golde, David W.; Bersch, Noelle; Chopra, Inder J.; Cline, Martin J.

doi:10.1111/j.1365-2141.1977.tb06833.x

Cited by 121 publications

(74 citation statements)

References 22 publications

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“…Low temperature-induced erythropoiesis in mice (Fandrey et al, 1994). In connection with hematopoiesis, earlier studies demonstrated that TH stimulates the proliferation and differentiation of erythrocyte progenitors in vivo and in vitro (Golde et al, 1977;Dainiak et al, 1978). From these reports and our results, it would appear that TH triggers renal Epo gene expression after low temperature exposure, and erythropoiesis is subsequently enhanced by EPO.…”

Section: Discussionsupporting

confidence: 65%

Enhanced erythropoiesis in mice exposed to low environmental temperature

Maekawa

Iemura

Kato

2012

Journal of Experimental Biology

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SUMMARYHematopoietic responses to environmental factors are not fully characterized. Polycythemia has been reported during exposure to low temperatures in ectothermic animals. The relationship between the causes of polycythemia and erythropoiesis during low temperature exposure is not fully understood. In this study, we exposed C57BL/6 mice to 5°C and monitored the blood cell counts and erythropoiesis. The hematocrit level increased from 45.6 to 52.2% after 14days. Likewise, the hemoglobin concentration, initially 15.1gdl . The reticulocyte production index significantly increased from 4 to 8% after 7days. We examined the anatomy and cell composition of the spleens of the mice. On day5, the spleens were ~6mgg −1 of body mass, which was twofold greater than the spleens on day0. Flow cytometry showed fourfold more proerythroblasts on day5, compared with day0. Additionally, the number of late-stage mature erythroblasts increased on day14. Erythropoietin mRNA levels increased in the kidneys, and hypoxia-inducible genes were enhanced in the kidney. Our findings indicated that low ambient temperature is a novel erythropoietic stress, which induces polycythemia by enhanced erythropoiesis.

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Section: Discussionsupporting

confidence: 65%

Enhanced erythropoiesis in mice exposed to low environmental temperature

Maekawa

Iemura

Kato

2012

Journal of Experimental Biology

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“…Furthermore, it has been postulated that the influence of THs on haematopoiesis involves an increased production of erythropoietin or haematopoietic factors by nonerythroid cells (10,11). However, a growing number of studies have demonstrated a direct role of THs in normal human and animal erythropoiesis (12)(13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Clinical relevance of thyroid dysfunction in human haematopoiesis: biochemical and molecular studies

Kawa¹,

Grymuła²,

Paczkowska

et al. 2010

European Journal of Endocrinology

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Objective: Abnormalities in haematological parameters have been noted in patients with thyroid diseases. Nevertheless, the exact mechanism of thyroid hormones' (THs) action on human haematopoiesis is still not entirely clear. Design: The influence of THs through TH receptors (TRa-1 and TRb-1) on haematopoiesis in patients with hypo-and hyperthyroidism was analysed. Methods: TR gene expression at the mRNA and protein levels in human CD34C -enriched haematopoietic progenitor cells (HPCs) obtained from the peripheral blood of patients with thyroid disorders and healthy volunteers was analysed. The cell populations were also investigated for clonogenic growth of granulocyte macrophage-colony forming units and erythrocyte-burst forming units (BFU-E). The level of apoptosis was determined by annexin V/propidium iodide staining and quantitative RT-PCR. Results: The studies revealed that hypo-and hyperthyroidism modify TR gene expression in HPCs in vivo. TH deficiency resulted in a decrease in total blood counts and clonogenic potential of BFU-E. In contrast, hyperthyroid patients presented increased clonogenic growth and BFU-E number and significantly higher expressions of cell cycle-regulating genes such as those for PCNA and cyclin D1. Finally, an increase in the frequency of apoptotic CD34C -enriched HPCs in hypo-and hyperthyroidism with a modulation of apoptosis-related genes was detected. Conclusions: The following conclusions were derived: i) TR expression in human haematopoietic cells depends on TH status, ii) both hypo-and hyperthyroidism significantly influence clonogenicity and induce apoptosis in CD34 C -enriched HPCs and iii) the molecular mechanism by which THs influence haematopoiesis might provide a basis for designing novel therapeutic interventions in thyroid diseases.

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“…Also iron clearance from the blood is reduced in hypothyroidism, suggesting reduced erythropoiesis. (15,17,18) The least common type of anaemia found in the present study was macrocytic anaemia (16%) and has been attributed to pernicious anaemia, decreased dietary intake and malabsorption of vit B12 and folic acid. Some studies have attributed much higher prevalence to this type of anaemia in hypothyroidism.…”

Section: Discussionmentioning

confidence: 86%