Thyrotropin-induced formation of functional follicles in primary cultures of ovine thyroid cells

Mak, Walter; Errick, Janice E.; Chan, Raymond K. H.; Eggo, Margaret C.; Burrow, Gerard N.

doi:10.1016/0014-4827(86)90031-5

Cited by 13 publications

(5 citation statements)

References 27 publications

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“…The porcine thyroid cells in this primary culture retain the following characteristics of thyroid follicular cells in the presence of TSH (11,15): three-dimensional follicle-like structure and iodide uptake like ovine thyroid cells in culture as reported previously (16).…”

Section: Effects Of Bile Acid On Iodide Uptakesupporting

confidence: 68%

Effects of Bile Acids on Iodide Uptake and Deoxyribonucleic Acid Synthesis in Porcine Thyroid Cells in Primary Culture

Kanri

Takiyama²,

Makino³

1996

Thyroid

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The effects of bile acids on iodide uptake and DNA synthesis were studied in cultured porcine thyroid cells. All five bile acids, which are commonly found in serum, chenodeoxycholic acid (CDCA), cholic acid (CA), deoxycholic acid (DCA), lithocholic acid (LCA), and ursodeoxycholic acid (UDCA) dose-dependently inhibited both basal and TSH-induced iodide uptake at concentrations of 25-250 microM. Since CDCA is one of the two major primary endogenous bile acids, were studied mainly the effects of CDCA. The inhibitory effect of CDCA was detected after 24 h treatment of thyroid cells, and was dependent on the time of exposure up to 72 h. Treatment of thyroid cells with CDCA for 72 h inhibited cAMP production stimulated by 50 mU/L TSH or 0.5 mg/L forskolin and also inhibited iodide uptake induced by 0.5 mM 8-bromo cAMP or 0.5 mg/L forskolin. These results suggest that CDCA inhibits iodide uptake by decreasing cAMP production as well as post-cAMP generation. Bile acids except LCA stimulated [3H]thymidine incorporation into the thyroid cells by itself, indicating that the inhibitory effect of bile acids on iodide uptake is not due to cytotoxic effect. In conclusion, these findings suggest that the direct inhibition of thyroid function by bile acids might cause hypothyroidism in chronic liver disease.

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Section: Effects Of Bile Acid On Iodide Uptakesupporting

confidence: 68%

Effects of Bile Acids on Iodide Uptake and Deoxyribonucleic Acid Synthesis in Porcine Thyroid Cells in Primary Culture

Kanri

Takiyama²,

Makino³

1996

Thyroid

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“…Although we have shown that TSH mediates many aspects of the differentiated function of sheep thyroid cells including follicle formation (Mak et al 1986a), protein glycosylation (Eggo & Burrow 1982), thyroglobulin synthesis (Errick et al 1985), iodide uptake and organification (Eggo et al 1984) and thyroid hormone synthesis (Becks et al 1986), we have not found TSH to be a growth factor. We have assayed for TSH effects on growth by cell counting, DNA content and thymidine uptake.…”

contrasting

confidence: 54%

Role of non-TSH factors in thyroid cell growth

Eggo

Bachrach

Burrow

1987

Acta Endocrinologica

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Abstract. The effects of insulin, the tumour promotor tetradecanoyl phorbol acetate (TPA), TSH and combinations of these factors on growth and DNA synthesis have been examined in the FRTL-5 cell strain and in sheep thyroid cells. In addition the regulation of the production by sheep thyroid cells of the insulin-like growth factors (IGF) by TSH and their possible autocrine roles have been investigated. We found that insulin and the IGF's stimulated DNA synthesis in both rat FRTL-5 cells and sheep cells. TPA also stimulated growth in both cell types, and its effects were additive to those of insulin. In the FRTL-5 cells, TPA was a less potent stimulator of growth than TSH, but the effects of TPA and TSH were not additive which may imply growth stimulation through a common pathway. In sheep cells TSH was not mitogenic and did not appear to activate protein kinase C, the receptor for TPA. Sheep cells, unlike FRTL-5 cells, were found to produce IGF-I and IGF-II, and their syntheses were regulated by TSH. Sheep cells were also found to produce IGF-binding proteins which may modulate the biologic effects of the IGF's. Sheep thyroid IGF binding proteins were found to copurify with urokinase-like plasminogen activator on immunoaffinity chromatography. The production of this serine protease has also been shown to be regulated by TSH.

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“…It is postulated that the dense granules may be intracellular storage deposits of the secretory product of the follicular cells (Gabe 1971, Henderson andGorbman 1971). Reabsorption products induced by thyroid stimulating hormone especially have been demonstrated by scanning electron microscopy (Balasse et al 1972, 1973, Avivi 1982, Mak et al 1986). 1972, 1973, Sobrinho-Simoes and Johannessen 1981.…”

Section: Discussionmentioning

confidence: 99%

“…In mammals it has been shown that these protrusions correspond to thyroglobulin secretory and reabsorption products. Reabsorption products induced by thyroid stimulating hormone especially have been demonstrated by scanning electron microscopy (Balasse et al 1972, 1973, Avivi 1982, Mak et al 1986). It appears likely that the cytoplasmic protrusions found on the apical surfaces of the hagfish thyroid follicles are thyroglobulin secretory and/or reabsorption products.…”

Section: Discussionmentioning

confidence: 99%

A Scanning Electron Microscopic Study on the Thyroid Follicle of the Hagfish Eptatretus burgeri

Suzuki

Kawabata

1988

Acta Zoologica

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Suzuki, S. and Kawabata, I. 1988. a scanning electron microscopic study on the thyroid follicle of the hagfish Eptatretus burgeri. Japan.)-Acta zool. (Stockh.) 69, 253-258.The external form of the follicles was mostly spherical or oval and the large oval follicles reached more than 300 X 180 pm in size. Cytoplasmic inclusions of various sizes were observed in the follicular epithelium. Follicles were composed of hexagonal epithelial cells, and on the apical surface of the cells many microvilli were found (380-500 microvilli per cell) with globular or amorphic cytoplasmic protrusions. In a few cells a central cilium was also observed. From these results a relationship between thyroid structure and function in the hagfish is discussed.

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Thyrotropin-induced formation of functional follicles in primary cultures of ovine thyroid cells

Cited by 13 publications

References 27 publications

Effects of Bile Acids on Iodide Uptake and Deoxyribonucleic Acid Synthesis in Porcine Thyroid Cells in Primary Culture

Effects of Bile Acids on Iodide Uptake and Deoxyribonucleic Acid Synthesis in Porcine Thyroid Cells in Primary Culture

Role of non-TSH factors in thyroid cell growth

A Scanning Electron Microscopic Study on the Thyroid Follicle of the Hagfish Eptatretus burgeri

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