THz irradiation inhibits cell division by affecting actin dynamics

Yamazaki, Shota; Ueno, Yuya; Hosoki, Ryosuke; Saito, Takanori; Idehara, T.; Yamaguchi, Yuusuke; Otani, Chiko; Ogawa, Yuichi; Harata, Masahiko; Hoshina, Hiromichi

doi:10.1101/2021.02.26.433024

Cited by 4 publications

(1 citation statement)

References 58 publications

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“…There exist conflicting reports with some authors suggesting that THz collapses actin filaments [28] while others claim an enhancement of actin fibrils lengthening dynamics [29]. It has also been observed that cellular division is inhibited via actin-mediated interferences [30].…”

Section: Interaction With Animal Tissue and Cellsmentioning

confidence: 99%

Introduction to the Biological Effects of Terahertz Radiation

Bucur-Portase¹

2023

Trends in Terahertz Technology

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Terahertz (THz) radiation has been noted to affect biological organisms to a unique degree with various effects ranging from modifications brought to protein activity to epigenetic changes that lead to altered metabolism or reproduction. These effects are classified into thermal and non-thermal, with the former being caused by THz’s capacity to induce localised thermal changes while the latter involves more complex interactions with cells’ macromolecules which are poorly understood. Terahertz’s ability to enhance actin polymerisation and alter gene expression leads to a number of possible applications in agriculture, as it has been observed that certain plant species have higher growth speeds post-exposure, and medicine, with cancer’s rapid division being possibly slowed down.

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Section: Interaction With Animal Tissue and Cellsmentioning

confidence: 99%

Introduction to the Biological Effects of Terahertz Radiation

Bucur-Portase¹

2023

Trends in Terahertz Technology

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Specific Features of the Proteomic Response of Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation

Bannikova

Khlebodarova

Vasilieva

et al. 2022

IJMS

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Studying the effects of terahertz (THz) radiation on the proteome of temperature-sensitive organisms is limited by a number of significant technical difficulties, one of which is maintaining an optimal temperature range to avoid thermal shock as much as possible. In the case of extremophilic species with an increased temperature tolerance, it is easier to isolate the effects of THz radiation directly. We studied the proteomic response to terahertz radiation of the thermophilic Geobacillus icigianus, persisting under wide temperature fluctuations with a 60 °C optimum. The experiments were performed with a terahertz free-electron laser (FEL) from the Siberian Center for Synchrotron and Terahertz Radiation, designed and employed by the Institute of Nuclear Physics of the SB of the RAS. A G. icigianus culture in LB medium was THz-irradiated for 15 min with 0.23 W/cm2 and 130 μm, using a specially designed cuvette. The life cycle of this bacterium proceeds under conditions of wide temperature and osmotic fluctuations, which makes its enzyme systems stress-resistant. The expression of several proteins was shown to change immediately after fifteen minutes of irradiation and after ten minutes of incubation at the end of exposure. The metabolic systems of electron transport, regulation of transcription and translation, cell growth and chemotaxis, synthesis of peptidoglycan, riboflavin, NADH, FAD and pyridoxal phosphate cofactors, Krebs cycle, ATP synthesis, chaperone and protease activity, and DNA repair, including methylated DNA, take part in the fast response to THz radiation. When the response developed after incubation, the systems of the cell’s anti-stress defense, chemotaxis, and, partially, cell growth were restored, but the respiration and energy metabolism, biosynthesis of riboflavin, cofactors, peptidoglycan, and translation system components remained affected and the amino acid metabolism system was involved.

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The Transcriptomic Response of Cells of the Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation

Peltek,

Bannikova,

Khlebodarova

et al. 2024

IJMS

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As areas of application of terahertz (THz) radiation expand in science and practice, evidence is accumulating that this type of radiation can affect not only biological molecules directly, but also cellular processes as a whole. In this study, the transcriptome in cells of the thermophilic bacterium Geobacillus icigianus was analyzed immediately after THz irradiation (0.23 W/cm2, 130 μm, 15 min) and at 10 min after its completion. THz irradiation does not affect the activity of heat shock protein genes and diminishes the activity of genes whose products are involved in peptidoglycan recycling, participate in redox reactions, and protect DNA and proteins from damage, including genes of chaperone protein ClpB and of DNA repair protein RadA, as well as genes of catalase and kinase McsB. Gene systems responsible for the homeostasis of transition metals (copper, iron, and zinc) proved to be the most sensitive to THz irradiation; downregulation of these systems increased significantly 10 min after the end of the irradiation. It was also hypothesized that some negative effects of THz radiation on metabolism in G. icigianus cells are related to disturbances in activities of gene systems controlled by metal-sensitive transcription factors.

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THz irradiation inhibits cell division by affecting actin dynamics

Cited by 4 publications

References 58 publications

Introduction to the Biological Effects of Terahertz Radiation

Introduction to the Biological Effects of Terahertz Radiation

Specific Features of the Proteomic Response of Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation

The Transcriptomic Response of Cells of the Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation

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