Ti3C2@Bi2O3 nanoaccordion for electrochemical determination of miRNA-21

Ouyang, Ruizhuo; Jiang, Lan; Xie, Xian-Jin; Yuan, Ping; Zhao, Yuefeng; Li, Yuhao; Tamayo, Abel Ibrahim Balbín; Liu, Baolin; Miao, Yuqing

doi:10.1007/s00604-022-05624-8

Cited by 16 publications

(3 citation statements)

References 44 publications

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“…Ouyang et al [55] constructed an electrochemical biosensor for miRNA-21 detection by combining nanomaterials and hybridization chain reaction (HCR). First, Ti 3 C 2 was obtained by etching Ti 3 AlC 2 with HF, and then Ti 3 C 2 was covered with Bi 2 O 3 nanoparticles to form Ti 3 C 2 @Bi 2 O 3 with an accordion-like structure.…”

Section: Voltammetrymentioning

confidence: 99%

MicroRNA Biosensors for Early Detection of Hepatocellular Carcinoma

Lin,

Wang,

Luo

et al. 2023

Chemosensors

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Hepatocellular carcinoma (HCC) is the main pathological type of liver cancer. Due to its insidious onset and the lack of specific early markers, HCC is often diagnosed at an advanced stage, and the survival rate of patients with partial liver resection is low. Non-coding RNAs (ncRNAs) have emerged as valuable biomarkers for HCC detection, with microRNAs (miRNAs) being a particularly relevant class of short ncRNAs. MiRNAs play a crucial role in gene expression regulation and can serve as biomarkers for early HCC detection. However, the detection of miRNAs poses a significant challenge due to their small molecular weight and low abundance. In recent years, biosensors utilizing electrochemical, optical, and electrochemiluminescent strategies have been developed to address the need for simple, rapid, highly specific, and sensitive miRNA detection. This paper reviews the recent advances in miRNA biosensors and discusses in detail the probe types, electrode materials, sensing strategies, linear ranges, and detection limits of the sensors. These studies are expected to enable early intervention and dynamic monitoring of tumor changes in HCC patients to improve their prognosis and survival status.

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Section: Voltammetrymentioning

confidence: 99%

MicroRNA Biosensors for Early Detection of Hepatocellular Carcinoma

Lin,

Wang,

Luo

et al. 2023

Chemosensors

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“…Ouyang et al fabricated a dual signal amplification strategy coupling accordion-like Bi 2 O 3 -decorated Ti 3 C 2 nanocomposites and HCR. Methylthionine chloride (MB) molecules were loaded on the final products, the electrochemical responses of which were then recorded for the evaluation of the miRNA level …”

mentioning

confidence: 99%

“…22 the electrochemical responses of which were then recorded for the evaluation of the miRNA level. 23 Besides commonly used nanomaterials, DNA itself serves as a type of powerful biomaterial to fabricate different nanostructures, which have broad prospects in the field of biosensing. 24 By rational designs, multiple ssDNA with specific base pairing are consumed from various DNA nanostructures like a DNA tetrahedron, DNA triangular prism (TPDNA), and DNA nanosphere.…”

mentioning

confidence: 99%

Irregular DNA Triangular Prism/Triplex Assembly for Duplicate MiRNA Analysis with Nicking Endonuclease-Mediated Amplification

2023

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Highly sensitive and selective detection of microRNA (miRNA) is becoming more and more important in the discovery, diagnosis, and prognosis of various diseases. Herein, we develop a three-dimensional DNA nanostructure based electrochemical platform for duplicate detection of miRNA amplified by nicking endonuclease. Target miRNA first helps construction of three-way junction structures on the surfaces of gold nanoparticles. After nicking endonuclease-powered cleavage reactions, single-stranded DNAs labeled with electrochemical species are released. These strands can be facilely immobilized at four edges of the irregular triangular prism DNA (iTPDNA) nanostructure via triplex assembly. By evaluating the electrochemical response, target miRNA levels can be determined. In addition, the triplexes can be disassociated by simply changing pH conditions, and the iTPDNA biointerface can be regenerated for duplicate analyses. The developed electrochemical method not only exhibits an excellent prospect in the detection of miRNA but also may inspire the engineering of recyclable biointerfaces for biosensing platforms.

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Efficient electrochemical immunosensor for detection of bovine interferon gamma‐based gold nanoparticles/chitosan composite

Wang,

Li,

Yang

et al. 2024

J Chinese Chemical Soc

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Interferon gamma (IFN‐γ) released by T lymphocytes, plays an important role in immune regulation and is also an important biomarker for the early diagnosis of bovine tuberculosis. In this work, an electrochemical immunosensor based on gold nanoparticles (AuNPs)/chitosan composite was developed for highly sensitive determination of bovine IFN‐γ. AuNPs/chitosan composite shows high surface area and good hydrophilicity. The bovine IFN‐γ immunosensor was prepared by immobilizing IFN‐γ antibody on AuNPs/chitosan composite‐modified glassy carbon electrode. The immunosensors were characterized by scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry (CV). Using horseradish peroxidase (HRP)‐labeled IFN‐γ antibody as amplification probe, the concentration of bovine IFN‐γ can be monitored via the increasing current signal, which can be attributed to the specific immune binding between bovine IFN‐γ antigen and HRP‐labeled IFN‐γ antibody, as well as the efficient catalysis reaction between thionine and HRP with the presence of H2O2. The developed electrochemical immunosensor exhibited a wide linear range of 0.01 to 30 ng/mL, and low detection limit of 0.003 ng/mL with good specificity and stability. This study provides a simple and promising method for diagnosis of bovine tuberculosis, which can be expanded to determine other biomarkers.

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Ti3C2@Bi2O3 nanoaccordion for electrochemical determination of miRNA-21

Cited by 16 publications

References 44 publications

MicroRNA Biosensors for Early Detection of Hepatocellular Carcinoma

MicroRNA Biosensors for Early Detection of Hepatocellular Carcinoma

Irregular DNA Triangular Prism/Triplex Assembly for Duplicate MiRNA Analysis with Nicking Endonuclease-Mediated Amplification

Efficient electrochemical immunosensor for detection of bovine interferon gamma‐based gold nanoparticles/chitosan composite

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