Tick-borne encephalitis virus in Mongolia

Isolation and characterization of tick-borne encephalitis virus from Ixodes persulcatus in Mongolia in 2012

“…Severe TBE cases have been reported since the 1980s in Selenge aimag and Bulgan aimag (near the border with Russia) (Frey et al, 2012). In Bulgan aimag, the viral genome was detected in a patient in 2008 and from ticks in 2010 and these viral genes were clustered within the Far-Eastern subtype and the Siberian subtype, respectively (Frey et al, 2012;Khasnatinov et al, 2010). However, minimal data are available concerning the biological characteristics of Mongolian TBEV strains (e.g., virulence and viral multiplication).…”

Fractionating the Plant Extract in Mini Volumes to Purify the Potential Antivirals from Terminalia Chebula

“…We used an isolate 92M of TBEV of the Siberian subtype. (15) Passaging of TBEV and determination of the concentration of infectious virus was carried out in the pig embryo kidney cell line (SPEV) purchased from the "Collection of human and animal cell lines for research in the field of virology" (FSI Influenza Research Institute, St. Petersburg, the Russian Federation). Cell culture was maintained on an RPMI1640 medium, supplemented with antibiotics and 5% fetal calf serum (ThermoScientific, UK).…”

Identification of the Siberian Type Tick-Borne Encephalitis Virus and Serological Surveillance in Mongolia

“…RT-PCR for detection of the TBEV E gene was performed with primers EncE-L (5'-GACCAGAGTGATCGAGGCTG-3') and 1643-R (5'-GCCAGATCATTRAACCAGTC-3') [9]. The mixture for RT-PCR was prepared using RNase-free water 13.3 ìêë, dNTP mixture 1.6 ìêë l, 10x buffer 2 ìêë l, F and R primers each 1 ìêë l, Ex Taq 0.1 ìêë l, and sample cDNA 1 ìêë l (final volume 20 ìêë l/sample).…”