2009
DOI: 10.1152/ajplung.00199.2009
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Tie2-mediated loss of peroxisome proliferator-activated receptor-γ in mice causes PDGF receptor-β-dependent pulmonary arterial muscularization

Abstract: Peroxisome proliferator-activated receptor (PPAR)-gamma is reduced in pulmonary arteries (PAs) of patients with PA hypertension (PAH), and we reported that deletion of PPARgamma in smooth muscle cells (SMCs) of transgenic mice results in PAH. However, the sequelae of loss of PPARgamma in PA endothelial cells (ECs) are unknown. Therefore, we bred Tie2-Cre mice with PPARgamma(flox/flox) mice to induce EC loss of PPARgamma (Tie2 PPARgamma(-/-)), and we assessed PAH by right ventricular systolic pressure (RVSP), R… Show more

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Cited by 144 publications
(133 citation statements)
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“…Our results in IP 2/2 mouse PASMCs favor the latter, although we cannot rule out the possibility in these experiments that GW9662 is acting in a non-PPARg-dependent manner because it enhanced PDGFinduced growth on its own. However, knockdown of PPARg in endothelial cells enhances lung PDGF receptor protein levels (30), and PPARg can oppose PDGF signaling in human PASMCs (18), thus providing a plausible explanation for the specificity of the observed effects of GW9662.…”
Section: Antiproliferative Mechanism Of Prostacyclin Analogsmentioning
confidence: 99%
“…Our results in IP 2/2 mouse PASMCs favor the latter, although we cannot rule out the possibility in these experiments that GW9662 is acting in a non-PPARg-dependent manner because it enhanced PDGFinduced growth on its own. However, knockdown of PPARg in endothelial cells enhances lung PDGF receptor protein levels (30), and PPARg can oppose PDGF signaling in human PASMCs (18), thus providing a plausible explanation for the specificity of the observed effects of GW9662.…”
Section: Antiproliferative Mechanism Of Prostacyclin Analogsmentioning
confidence: 99%
“…27 Briefly, RV systolic pressure, RV dp/dt, and heart rate were measured in unventilated mice that were under isoflurane anesthesia (1.5% to 2.5%, 2 L O 2 /min) using a closed chest technique, by introducing a 1.4-F Millar catheter into the jugular vein and directing it to the right ventricle. The lungs were then flushed with warm isotonic saline through a vented left atrium to clear blood, and the heart and lungs were removed en bloc.…”
Section: Hemodynamic and Structural Evaluation Of Pahmentioning
confidence: 99%
“…33 Similarly, PAH developed in mice with SMC-or endothelial cellspecific deletion of PPARγ. 34,35 In contrast, PPARγ activation ameliorated PAH. [32][33][34][36][37][38] However, the effect of PPARγ agonists on the vasoconstriction in PAH remains unknown.…”
mentioning
confidence: 97%