Tigecycline resistance in Acinetobacter baumannii mediated by frameshift mutation in plsC, encoding 1-acyl-sn-glycerol-3-phosphate acyltransferase

X, Li; Liu, Lei; J, Ji; Chen, Q; Hua, Xin; Jiang, Yan; Feng, Youjun; Yang, Yu

doi:10.1007/s10096-014-2272-y

Cited by 50 publications

(33 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Log-phase bacterial cultures were diluted to approximately 1 ϫ 10 6 cells/ml in filtered PBS without washing. Membrane potential (MP) was measured as described previously (38). Membrane permeability was measured by 100 nM To-Pro-3 (Invitrogen, Thermo Fisher Scientific, USA), which stains nucleic acids in cells when the membrane is damaged.…”

Section: Methodsmentioning

confidence: 99%

Dual Role of gnaA in Antibiotic Resistance and Virulence in Acinetobacter baumannii

Chen

Yan

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Acinetobacter baumannii is an important Gram-negative pathogen in hospital-related infections. However, treatment options for A. baumannii infections have become limited due to multidrug resistance. Bacterial virulence is often associated with capsule genes found in the K locus, many of which are essential for biosynthesis of the bacterial envelope. However, the roles of other genes in the K locus remain largely unknown. From an in vitro evolution experiment, we obtained an isolate of the virulent and multidrug-resistant A. baumannii strain MDR-ZJ06, called MDR-ZJ06M, which has an insertion by the ISAba16 transposon in gnaA (encoding UDP-N-acetylglucosamine C-6 dehydrogenase), a gene found in the K locus. The isolate showed an increased resistance toward tigecycline, whereas the MIC decreased in the case of carbapenems, cephalosporins, colistin, and minocycline. By using knockout and complementation experiments, we demonstrated that gnaA is important for the synthesis of lipooligosaccharide and capsular polysaccharide and that disruption of the gene affects the morphology, drug susceptibility, and virulence of the pathogen.

show abstract

Section: Methodsmentioning

confidence: 99%

Dual Role of gnaA in Antibiotic Resistance and Virulence in Acinetobacter baumannii

Chen

Yan

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…The OD 600 of each culture was determined every 5 min for 16 h using a the BioTEK Synergy plate reader (Biotek, Winooski, VT, USA). The growth rate was estimated by an R script based on the OD 600 curves (Li et al, 2015). Fitness data are shown in the Supplementary Table S1 .…”

Section: Methodsmentioning

confidence: 99%

The Effect of Colistin Resistance-Associated Mutations on the Fitness of Acinetobacter baumannii

Wang

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

Acinetobacter baumannii had emerged as an important nosocomial and opportunistic pathogen worldwide. To assess the evolution of colistin resistance in A. baumannii and its effect on bacterial fitness, we exposed five independent colonies of A. baumannii ATCC 17978 to increasing concentrations of colistin in agar (4/5) and liquid media (1/5). Stable resistant isolates were analyzed using whole genome sequencing. All strains were colistin resistant after exposure to colistin. In addition to the previously reported lpxCAD and pmrAB mutations, we identified four novel putative colistin resistance genes: A1S_1983. hepA. A1S_3026, and rsfS. Lipopolysaccharide (LPS) loss mutants exhibited higher fitness costs than those of the pmrB mutant in nutrient-rich medium. The colistin-resistant mutants had a higher inhibition ratio in the serum growth experiment than that of the wild type strain in 100% serum. Minimum inhibitory concentration (MIC) results showed that the LPS-deficient but not the pmrB mutant had an altered antibiotic resistance profile. The compensatory mutations partially or completely rescued the LPS-deficient’s fitness, suggesting that compensatory mutations play an important role in the emergence and spread of colistin resistance in A. baumannii.

show abstract

“…However, its exact mechanism was not determined. Another study revealed that a frameshift mutation in plsC , encoding 1-acyl- sn -glycerol-3-phosphate acyltransferase, is associated with decreased susceptibility to tigecycline (Li X. et al, 2015). …”

Section: Antimicrobial Resistance Of a Baumanniimentioning

confidence: 99%

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Lee

Park

et al. 2017

Front. Cell. Infect. Microbiol.

755

585

View full text Add to dashboard Cite

Acinetobacter baumannii is undoubtedly one of the most successful pathogens responsible for hospital-acquired nosocomial infections in the modern healthcare system. Due to the prevalence of infections and outbreaks caused by multi-drug resistant A. baumannii, few antibiotics are effective for treating infections caused by this pathogen. To overcome this problem, knowledge of the pathogenesis and antibiotic resistance mechanisms of A. baumannii is important. In this review, we summarize current studies on the virulence factors that contribute to A. baumannii pathogenesis, including porins, capsular polysaccharides, lipopolysaccharides, phospholipases, outer membrane vesicles, metal acquisition systems, and protein secretion systems. Mechanisms of antibiotic resistance of this organism, including acquirement of β-lactamases, up-regulation of multidrug efflux pumps, modification of aminoglycosides, permeability defects, and alteration of target sites, are also discussed. Lastly, novel prospective treatment options for infections caused by multi-drug resistant A. baumannii are summarized.

show abstract

Tigecycline resistance in Acinetobacter baumannii mediated by frameshift mutation in plsC, encoding 1-acyl-sn-glycerol-3-phosphate acyltransferase

Cited by 50 publications

References 32 publications

Dual Role of gnaA in Antibiotic Resistance and Virulence in Acinetobacter baumannii

Dual Role of gnaA in Antibiotic Resistance and Virulence in Acinetobacter baumannii

The Effect of Colistin Resistance-Associated Mutations on the Fitness of Acinetobacter baumannii

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Contact Info

Product

Resources

About