The tiger beetles of the southern Levant (Egypt: Sinai, Israel, Jordan) and adjacent regions of the neighboring countries Lebanon, Syria, Iraq, Saudi Arabia and Egypt are reviewed in terms of species taxonomy, ecological and distributional traits and conservation biology. An illustrated dichotomous identification key from the species of this region is provided. Based on the classical identification key, we developed a digital identification application for smartphones and tablets. The species status of Calomera
aulicoides (J.R. Sahlberg, 1913) is (re-) established (stat. rest.) as this taxon can be found sympatrically and parapatrically together with Calomera
littoralis
winkleri (Mandl, 1934). Morphological character states are discussed to identify Cicindela
javetii Chaudoir, 1861 and C.
herbacea Klug, 1832. Calomera
aphrodisia (Baudi di Selve, 1864) is recorded for the first time from Israel. The presence of Calomera
aulica (Dejean, 1831) and Grammognatha
euphratica (Dejean, 1822) is confirmed by new records. At least five taxa are threatened or extinct in Israel. For one of these species, Israel has a national responsibility for the conservation as the main part of the distribution range is within this country.Availability: The application TIGER BEETLE ID for Android devices can be freely downloaded at https://doi.org/10.3897/zookeys.734.21989.suppl1. See also disclaimer of warranties.