Tight Junction Disruption and Recovery after Sublethal γ Irradiation

Porvaznik, Martin

doi:10.2307/3575041

Cited by 59 publications

(16 citation statements)

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“…However, there are indications for TJs' roles in the cellular and pathological processes following exposure to radiation. For instance, TJs in MDCK cells exposed to ionizing irradiation were disassembled [60,61]. Recently, an interesting investigation by Zitzelsberger et al [62] showed that Par3 gene was strongly amplified in radiation-transformed retinal pigment epithelial cell lines, suggesting that Par3 may function in radiation-induced carcinogenesis.…”

Section: Intercellular Junctions and Dna Damage Repairmentioning

confidence: 99%

Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair

Fang

Wang

et al. 2007

Cell Res

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Section: Intercellular Junctions and Dna Damage Repairmentioning

confidence: 99%

Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair

Fang

Wang

et al. 2007

Cell Res

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“…However, increased intestinal permeability was also found at irradiation doses that do not induce denudation of intestinal epithelium. For instance, an increased passage of tracer in extracellular spaces was observed in rat ileum following 5 Gy (Porvaznik 1979;Somosy et al 1993).…”

Section: Introductionmentioning

confidence: 92%

“…The role of adherent junctions in cell-cell adhesion is complementary to that of tight junctions. Porvaznik was one of the first to postulate the role of tight junctions in the radiation-induced epithelial barrier disruption (Porvaznik 1979). Thus, one of the hypotheses attempting to explain the increase of intestinal permeability observed after radiation exposure is a loss of "epithelial tightness" owing to a disorganization of junctional complexes of intestinal epithelium.…”

Section: Introductionmentioning

confidence: 99%

Functional and structural alterations of epithelial barrier properties of rat ileum following X-irradiation

Dublineau¹,

Lebrun²,

Grison³

et al. 2004

Can. J. Physiol. Pharmacol.

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Irradiation of the digestive system leads to alterations of the small intestine. We have characterized the disruption of the barrier integrity in rat ileum from 1 to 14 days following irradiation ranging from 6 to 12 Gy. The intestinal permeability to 14C-mannitol and 3H-dextran 70 000 was measured in vitro in Ussing chambers. In parallel to these functional studies, immunohistochemical analyses of junctional proteins (ZO-1 and beta-catenin) of ileal epithelium were performed by confocal microscopy. Irradiation with 10 Gy induced a marked decrease in epithelial tissue resistance at three days and a fivefold increase in mannitol permeability, without modifications of dextran permeability. A disorganization of the localization for ZO-1 and beta-catenin was also observed. At 7 days after irradiation, we observed a recovery of the organization of junctional proteins in parallel to a return of intestinal permeability to control value. In addition to these time-dependent effects, a gradual effect on epithelial integrity of the radiation doses was observed 3 days after irradiation. This study shows a disruption of the integrity of the intestinal barrier in rat ileum following abdominal X-irradiation, depending on the time postirradiation and on the delivered dose. The loss of barrier integrity was characterized by a disorganization of proteins of tight and adherent junctions, leading to increased intestinal permeability to mannitol.

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“…Large macular-tight junctions are present on the lateral region of the plasma mem brane. Similar tight junction alterations have been described in other experi mental systems using physiological, pharmacological and pathological stimuli (Orci et aL, 1973;Bulger etaL, 1974;Goodenoughand Gilula, 1974;77ce et aL, 1975;Elias and Friend, 1976;Humpert et aL, 1976: Montesano et aL, 1976Bouchaud and Bouvier, 1978\ Meldolesi et aL, 1978,Porvaznik, 1979, 1980. The previous descriptions of tight junction alterations offered a number of speculations concerning the mechanism of their unusual formation.…”

Section: Freeze Fracturementioning

confidence: 82%

A Correlated Thin Section and Freeze-Fracture Study of <i>o</i>-Phenylphenol-Induced Alterations in the Rat Liver

Robenek¹,

Meiß²,

Themann³

et al. 1980

Pathobiology

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The ultrastructural changes in the liver cells of male Wistar rats induced by oral and subcutaneous application of o-phenylphenol were studied electron microscopically using the thin section and freeze-fracture technique. The rats were given the o-phenylphenol once at a dose of 2,500 mg/kg b.w. and were sacrificed 60 h after treatment. In the orally treated animals, alterations in the nuclei and nucleoli of the hepatocytes were the most prominent alterations. Furthermore, an increase in smooth endoplasmic reticulum, lysosomes and microbodies were found. After the subcutaneous injection of o-phenylphenol enlargements of the bile canaliculi, intercellular space and pathological alterations in the mitochondria were seen. A proliferation of the smooth endoplasmic reticulum, an increase of microbodies and a decrease in the rough endoplasmic reticulum could be found. Both groups showed an increase in small intracellular fat droplets in the hepatocytes. In general, the effects of the subcutaneous application on the liver were more pronounced than the effects of the oral application. Freeze-fracture replicas exhibited a disorganization of the zonulae occludentes and an apparent increase in the number and size of gap junctions. These alterations were interpreted as an attempt of the liver cells to counteract the intracanalicular pressure and to increase the mechanical stability of the liver tissue.

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Tight Junction Disruption and Recovery after Sublethal γ Irradiation

Cited by 59 publications

References 0 publications

Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair

Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair

Functional and structural alterations of epithelial barrier properties of rat ileum following X-irradiation

A Correlated Thin Section and Freeze-Fracture Study of <i>o</i>-Phenylphenol-Induced Alterations in the Rat Liver

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