Glued wood is one of the most commonly used materials made of wood. Glued wood has many advantages related to its strength characteristics and operation. Nevertheless, due to the use of an adhesive base, it becomes necessary to carefully approach the issue of the fire resistance of building structures made of glued wood. The purpose of this study was to assess the effect of structural fire protection on the fire resistance of glued laminated timber columns; the task of developing methods for experimental and analytical assessments of the fire resistance of glued laminated timber columns, with the possibility of assessing the temperature of the wood under a layer of fire protection, was set, and an analysis of the effectiveness of these methods for assessing the fire resistance of such structures was conducted. The experimental assessment of fire resistance was based on the combined effects of fire and force on structures. The analytical assessment of the fire resistance was carried out using two methods, each of which estimated the time of the beginning of the ignition of the wood, as well as its combustion before the limit state of the structure was reached, but did not ascertain the acting force. As a result of evaluating the effect of structural fire protection on the fire resistance of glued wood columns, data on the heating of wood under a layer of fire protection were obtained, and the relationship between the deformation of the sample and the heating of the layers of fire protection was revealed, consisting of an increase in the ignition time of the wood with an increase in the thickness of the fire protection.