Time-alignment of electrical network measurements through time series of cycle RMS values

Souza, Geraldo Pereira de; Boaventura, Wallace do Couto

doi:10.1016/j.ijepes.2022.108518

Cited by 3 publications

(4 citation statements)

References 40 publications

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“…The i−t traces and fluorescence−time traces were aligned to within 0.5 ms using cross-correlation time alignment methods. 17 The time at which liposomes entered the nanopipet was cross-correlated with the time associated with the onset of the fluorescence signal during the liposome multipassing to perform the alignment.…”

Section: Methodsmentioning

confidence: 99%

“…Coulter-counter or resistive-pulse sensing is widely used to characterize particle size, charge, shape, and concentration. − Resistive-pulse sensing was introduced in 1951 as an automated technique to count and size red blood cells and is still commonly used today. − In resistive-pulse sensing, a cell or particle is detected as it passes through an opening separating two ionically conductive solutions by partially blocking the electrical current established between the electrodes placed on each side of the opening. In this work, the orifice of a laser-pulled glass nanopipet is used to resistively sense the translocations of liposomes as they enter and leave the nanopipet.…”

mentioning

confidence: 99%

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Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

Schmeltzer,

Peterson,

Harris

et al. 2024

ACS Nano

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Simultaneous multipass resistive-pulse sensing and fluorescence imaging have been used to correlate the size and fluorescence intensity of individual E. coli lipid liposomes composed of E. coli polar lipid extracts labeled with membranebound 3,3-dioctadecyloxacarbocyanine (DiO) fluorescent molecules. Here, a nanopipet serves as a waveguide to direct excitation light to the resistive-pulse sensing zone at the end of the nanopipet tip. Individual DiO-labeled liposomes (>50 nm radius) were multipassed back and forth through the orifices of glass nanopipets' 110−150 nm radius via potential switching to obtain subnanometer sizing precision, while recording the fluorescence intensity of the membrane-bound DiO molecules. Fluorescence was measured as a function of liposome radius and found to be approximately proportional to the total membrane surface area. The observed relationship between liposome size and fluorescence intensity suggests that multivesicle liposomes emit greater fluorescence compared to unilamellar liposomes, consistent with all lipid membranes of the multivesicle liposomes containing DiO. Fluorescent and nonfluorescent liposomes are readily distinguished from each other in the same solution using simultaneous multipass resistive-pulse sensing and fluorescence imaging. A fluorescence "dead zone" of ∼1 μm thickness just outside of the nanopipet orifice was observed during resistive-pulse sensing, resulting in "on/off" fluorescent behavior during liposome multipassing.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

Schmeltzer,

Peterson,

Harris

et al. 2024

ACS Nano

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show abstract

“…The i-t traces and fluorescence-time traces were aligned to within 0.5 ms using crosscorrelation time alignment methods. 17 The time at which liposomes entered the nanopipette was…”

Section: Data Processingmentioning

confidence: 99%

“…Coulter-counter or resistive-pulse sensing is widely used to characterize particle size, charge, shape, and concentration. [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] Resistive-pulse sensing was introduced in 1951 as an automated technique to count and size red blood cells, and is still commonly used today. [20][21][22][23][24][25][26] In resistive-pulse sensing, a cell or particle is detected as it passes through an opening separating two ionically conductive solutions by partially blocking the electrical current established between the electrodes placed on each side of the opening.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

Schmeltzer,

Peterson,

Lathrop

et al. 2023

Preprint

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Simultaneous multipass resistive-pulse sensing and fluorescence imaging have been used to correlate the size and fluorescence intensity of individual E. coli lipid liposomes composed of E. coli polar lipid extract labeled with membrane-bound 3,3-dioctadecyloxacarbocyanine (DiO) fluorescent molecules. Here, a nanopipette serves as a waveguide to direct excitation light to the resistive-pulse sensing zone at the end of the nanopipette tip. Individual DiO-labeled liposomes (>50 nm radius) were multipassed back and forth through the orifices of glass nanopipettes 110-to-150 nm radius via potential switching to obtain sub-nanometer sizing precision, while recording the fluorescence intensity of the membrane-bound DiO molecules. Fluorescence was measured as a function of liposome radius and found to be approximately proportional to the total membrane surface area. The observed relationship between liposome size and fluorescence intensity suggests that multi-vesicle liposomes emit greater fluorescence compared to unilamellar liposomes, consistent with all lipid membranes of the multi-vesicle liposomes containing DiO. Fluorescent and non-fluorescent liposomes are readily distinguished from each other in the same solution using simultaneous multipass resistive-pulse sensing and fluorescence imaging. A fluorescence ‘dead zone’ of ~1 m thickness just outside of the nanopipette orifice was observed during resistive-pulse sensing, resulting in ‘on/off’ fluorescent behavior during liposome multipassing. Our results provide a path forward to simultaneously characterize the size of biologically relevant nanoparticles (e.g., extracellular vesicles) and the presence of fluorescently labeled surface proteins.

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Hybrid state-estimation in combined heat and electric network using SCADA and AMI measurements

Srinivas,

Wu,

Singh

et al. 2024

International Journal of Electrical Power & Energy Systems

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Time-alignment of electrical network measurements through time series of cycle RMS values

Cited by 3 publications

References 40 publications

Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

Hybrid state-estimation in combined heat and electric network using SCADA and AMI measurements

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