Time and frequency domain deconvolution for cross-sectional cultured cell observation using an acoustic impedance microscope

Prastika, Edo Bagus; T, Shintani; Kawashima, T.; Murakami, Yoshinobu; Hozumi, Naohiro; Soon, Thomas Tiong Kwong; Nagaoka, Ryo; Kobayashi, Kazuto

doi:10.1016/j.ultras.2021.106601

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…5,7,8,11,12,30,31) While conventional optical microscopy also provides us with information on the cell morphology, SAM was able to provide us with the changes of the acoustic impedance value of cells upon drug treatment, which is infeasible in optical microscopy. 6,9,10,13) This indicates that SAM has the potential to provide more information in cell study as compared to optical microscopy.…”

Section: Discussionmentioning

confidence: 99%

“…The cell was seeded on a polystyrene (PS) film dish (Honda Electronics Co. Ltd., Japan) as described previously. 6,9,10,13) Briefly, the cell was cultured at an initial density of 7000 ∼ 15 000 cells cm −2 and incubated at 37 °C in a 5% CO 2 atmosphere. The cell was proliferated in culture media containing Dulbecco's Modified Eagle's Medium nutrient mixture F-12 (DMEM/F-12) supplemented with 10% immobilized fetal bovine serum and 1% Kanamycin (Wako Pure Chemical Industries).…”

Section: Breast Cancer Cell Culturementioning

confidence: 99%

“…The specimen was subjected to waveform acquisition eight times for noise reduction by averaging. 13) Figure 1(b) illustrates the signals used for calculation of the acoustic impedance as described in a previous paper. 16) The reference signal, denoted by S ref , is the reflection signal obtained from the interface between the substrate, which is the PS film, and the water, while the target signal, denoted by S tgt , is the signal obtained from the interface between the substrate and the cell.…”

Section: Scanning Acoustic Microscopymentioning

confidence: 99%

“…First, living observation is feasible as the acoustic wave generated from the transducer in order to map the cells is non-invasive. [3][4][5][6][7][8][9][10][11][12][13] Next, the dynamics of cellular morphology correspond with the cell elasticity and could give us new insight into the changes in cellular mechanical properties which are often overlooked. 6,[9][10][11]13,14) These benefits are unattainable with current techniques for studying breast cancer, which often involve invasive methods such as biopsy.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of elastic change during the mitotic phase of murine breast cancer cells using scanning acoustic microscopy

Soon

Sasaki

Prastika

et al. 2022

Jpn. J. Appl. Phys.

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Scanning acoustic microscope (SAM) is a useful observational tool in cellular study as living observation which is non-invasive is feasible compared to conventional optical microscope. In previous study, the cell morphological change by acoustic impedance measurement were successfully visualized. These acoustic impedance changes correspond with cell elasticity mainly reflects the changes of the cytoskeleton. In this study, we evaluate the elastic change of murine breast cancer cell, C127I during mitosis. C127I cells were cultured to ~75% confluency before measurement, using a transducer with a central frequency of 320 MHz. Dynamic changes during mitosis were successfully mapped using SAM and confirmed by laser confocal microscopy. Cells in prometaphase, anaphase and telophase which can only be confirmed through immunostaining before were successfully visualized using SAM. This suggests that SAM is capable to distinguish cell in different mitotic phase based on the changes of acoustic impedance.

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Section: Discussionmentioning

confidence: 99%

Section: Breast Cancer Cell Culturementioning

confidence: 99%

Section: Scanning Acoustic Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of elastic change during the mitotic phase of murine breast cancer cells using scanning acoustic microscopy

Soon

Sasaki

Prastika

et al. 2022

Jpn. J. Appl. Phys.

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“…Calculation of the acoustic impedance, of which details have been described in previous papers [ 1 , 2 ], is illustrated in Fig. 1 c. For C-mode, the acoustic impedance is obtained by comparing reflection intensity directly with a reference signal.…”

Section: Acoustic Cell Observation and Calculationmentioning

confidence: 99%

Noninvasive imaging of rat-derived microglia and its reactivity to inflammatory molecules via acoustic impedance microscopy

Lee,

Yap,

Umemura

et al. 2023

J Med Ultrasonics

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Purpose Microglia, the brain's immune cells, play important roles in neuronal differentiation, survival, and death. The function of microglia is deeply related to the morphologies; however, it is too complex to observe conventionally and identify the condition of living microglia using optical microscopes. Herein, we proposed a new method to observe living cultured microglia and their reactivity to inflammation via the acoustic impedance mode of a scanning acoustic microscope. Methods Primary cultured microglia collected from rat pups exposed to acetamiprid, an insecticide, in utero were observed with both acoustic interface impedance mode (C-mode) and transparent three-dimensional impedance mode (B-mode). Results We characterized microglia into four types based on the results obtained from acoustic impedance, cytoskeletal information, and laser confocal imaging. Biphasic acoustic observation using B-mode and C-mode gave us information regarding the dynamic morphologies of living microglia treated with adenosine triphosphate (ATP) (600 μmol/L), which reflects distress signals from inflamed neurons. Acetamiprid exposure induced microglia response even in the neonatal period. ATP stimulus altered the shape and thickness of microglia with a change in the bulk modulus of the cell. Three-dimensional alteration with ATP stimulus could be observed only after biphasic acoustic observation using B-mode and C-mode. This acoustic observation was consistent with confocal observation using anti-Iba-1 and P2Y12 immunocytochemistry. Conclusion This study demonstrated the adequacy of using a scanning acoustic microscope in analyzing microglia's shape, motility, and response to inflammation.

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Singular value decomposition with weighting matrix applied for optical-resolution photoacoustic microscopes

Sulistyawan,

Nishimae,

Ishii

et al. 2024

Ultrasonics

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Time and frequency domain deconvolution for cross-sectional cultured cell observation using an acoustic impedance microscope

Cited by 5 publications

References 32 publications

Evaluation of elastic change during the mitotic phase of murine breast cancer cells using scanning acoustic microscopy

Evaluation of elastic change during the mitotic phase of murine breast cancer cells using scanning acoustic microscopy

Noninvasive imaging of rat-derived microglia and its reactivity to inflammatory molecules via acoustic impedance microscopy

Singular value decomposition with weighting matrix applied for optical-resolution photoacoustic microscopes

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