The Satellite Laser Ranging (SLR) technique provides very accurate distance measurements to artificial Earth satellites. SLR is employed for the realization of the origin and the scale of the terrestrial reference frame. Despite the high precision, SLR observations can be affected by various systematic errors. So far, range biases were used to account for systematic measurement errors and mismodeling effects in SLR. Range biases are constant for all elevation angles and independent of the measured distance to a satellite. Recently, intensity-dependent biases for single-photon SLR detectors and offsets of barometer readings and meteorological devices were reported for some SLR stations. In this paper, we study the possibility of the direct estimation of tropospheric biases from SLR observations to LAGEOS satellites. We discuss the correlations between the station heights, range biases, tropospheric biases, and their impact on the repeatability of station coordinates, geocenter motion, and the global scale of the reference frame. We found that the solution with the estimation of tropospheric biases provides more stable station coordinates than the solution with the estimation of range biases. From the common estimation of range and tropospheric biases, we found that most of the systematic effects at SLR stations are better absorbed by elevation-dependent tropospheric biases than range biases which overestimate the total bias effect. The estimation of tropospheric biases changes the SLR-derived global scale by 0.3 mm and the geocenter coordinates by 1 mm for the Z component, causing thus an offset in the realization of the reference frame origin. Estimation of range biases introduces an offset in some SLR-derived low-degree spherical harmonics of the Earth’s gravity field. Therefore, considering elevation-dependent tropospheric and intensity biases is essential for deriving high-accuracy geodetic parameters.