Time and temperature effects on potassium concentration of stored whole blood from four mammalian species

S, Itó; Matsuzawa, Toshiaki; Saida, M.; Izumisawa, S.; Iwasaki, Mai

doi:10.1007/bf02642495

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…The interchangeability of serum and different plasmas for biochemistry profiles is somewhat surprising, as clot formation results in the modification of the concentration of some analytes. For instance, intracellular molecules such as potassium can leak from cells, as a consequence, potassium concentration can be lower in plasma than in serum in species with high intracellular potassium levels or in cases with thrombocytosis . At the same time, clot formation can also result in sequestration of analytes; for instance, mean copper concentration is about 25% lower in serum than plasma of ruminants, although the interindividual variability appears considerable .…”

Section: Technical Preanalytic Factorsmentioning

confidence: 99%

The preanalytic phase in veterinary clinical pathology

Braun

Bourgès‐Abella

Geffré

et al. 2014

Veterinary Clinical Pathol

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This article presents the general causes of preanalytic variability with a few examples showing specialists and practitioners that special and improved care should be given to this too often neglected phase. The preanalytic phase of clinical pathology includes all the steps from specimen collection to analysis. It is the phase where most laboratory errors occur in human, and probably also in veterinary clinical pathology. Numerous causes may affect the validity of the results, including technical factors, such as the choice of anticoagulant, the blood vessel sampled, and the duration and conditions of specimen handling. While the latter factors can be defined, influence of biologic and physiologic factors such as feeding and fasting, stress, and biologic and endocrine rhythms can often not be controlled. Nevertheless, as many factors as possible should at least be documented. The importance of the preanalytic phase is often not given the necessary attention, although the validity of the results and consequent clinical decision making and medical management of animal patients would likely be improved if the quality of specimens submitted to the laboratory was optimized.

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Section: Technical Preanalytic Factorsmentioning

confidence: 99%

The preanalytic phase in veterinary clinical pathology

Braun

Bourgès‐Abella

Geffré

et al. 2014

Veterinary Clinical Pathol

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“…plasma and erythrocytes) must be conducted under laboratory conditions prior to freezer storage, samples are often stored on ice while in the field and subsequently transported to the laboratory after a number of hours with the expectation that the blood properties do not change significantly in the interim. Although some studies exist on the effects of storage on the blood properties of fishes and other vertebrates (Lumeij, 1985; Korcock et al , 1988; Nielsen & Lykkeboe, 1992; Ito et al , 1998; Reece et al , 2006), inconsistent findings emphasize the need for further research on new species.…”

Section: Introductionmentioning

confidence: 99%

The efficacy of field techniques for obtaining and storing blood samples from fishes

Clark

Donaldson

Drenner

et al. 2011

Journal of Fish Biology

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Prompted by the dramatic increase in the use of blood analyses in fisheries research and monitoring, this study investigated the efficacy of common field techniques for sampling and storing blood from fishes. Three questions were addressed: (1) Do blood samples taken via rapid caudal puncture (the 'grab-and-stab' technique) yield similar results for live v. sacrificed groups of fishes? (2) Do rapidly obtained caudal blood samples accurately represent blood properties of fishes prior to capture? (3) Does storage of whole blood in an ice slurry for a working day (8·5 h) modify the properties of the plasma? It was shown that haematocrit, plasma ions, metabolites, stress hormones and sex hormones of caudal blood samples were statistically similar when taken from live v. recently sacrificed groups of adult coho salmon Oncorhynchus kisutch. Moreover, this study confirmed by using paired blood samples from cannulated O. kisutch that blood acquired through the caudal puncture technique (mean ±s.e. 142 ± 26 s after capture) was representative of fish prior to capture. Long-term (8·5 h) cold storage of sockeye salmon Oncorhynchus nerka whole blood caused significant decreases in plasma potassium and chloride, and a significant increase in plasma glucose. Previous research has suggested that these changes largely result from net movements of ions and molecules between the plasma and erythrocytes, movements that can occur within minutes of storage. Thus, blood samples from fishes should be centrifuged as quickly as practicable in the field for separation of plasma and erythrocytes to prevent potentially misleading data.

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