Time-based Elution of TEGDMA and BisGMA from Resin Composite Cured with LED, QTH and High-intensity QTH Lights

Abstract

A Nalçaci • N Ulusoy • O Atakol Clinical RelevanceUnder the conditions of this study, when compared to standard QTH, both standard LED and high-intensity QTH light curing resulted in lower levels of the elution of TEGDMA, which is suspected to be the prime cause of cytotoxic reactions in resin composite restorations. SUMMARYThis study measured the elution of TEGDMA and BisGMA monomers from hybrid, micro-filled resin composites over 72 hours at different time intervals after polymerization with standard quartz-tungsten-halogen (QTH), high-intensity fast-curing QTH and standard blue light emitting diode (LED) light units. Samples were polymerized from the top and bottom surfaces, then immersed in methanol. High performance liquid chromatography (HPLC) was used to measure the amount of monomers released from the samples at various time intervals, ranging from 0 to 72 hours (0, 3, 6, 9, 12, 24, 48 and 72 hours). Data was analyzed using two-way ANOVA and Duncan tests with a significance level of 0.05. No significant differences were observed among curing groups in the elution of TEGDMA monomers at 0, 9, 12, 24, 48 and 72 hours; whereas, significant differences were observed among curing groups at 3 and 6 hours. BisGMA elution in samples immersed for longer periods (9-72 hours) were significantly higher than samples immersed for shorter time periods (0-6 hours); however, 72 hours appeared to be too short a period for the total elution of BisGMA into methanol.

Degree of conversion and leached monomers of urethane dimethacrylate-hydroxypropyl methacrylate-based dental resin systems

“…In the current study [ Fig. 5 (a-d)], all the weight percentage values of the eluted monomers were under 3.5%, which was in agreement with some previous studies (14,15,24,25).…”

“…Apart from the relatively bigger molecular weight, bis-GMA is comprised of two phenyl groups, making it a bulky three-dimensional molecule. This may also explain bis-GMA's slower and lesser elution compared to MMA (25).…”

In vitro potential cytotoxicity of an adhesive system to alveolar macrophages

“…Considerable reduction in the substances capable of being lixiviated was recorded around the 7th day of immersion (20); this could indicate that in this period, complete lixiviation of the free monomers does not occur (19). Although these substances can still be detected after 7 days, cellular viability is not affected by the extracts at 5 or more days (20).…”

“…The extracts used in the present study were obtained by immersing the samples in the culture medium for either 24 or 72 h, which is a sufficient period for 85% of the unreacted monomer Bis-GMA to be released (19). Considerable reduction in the substances capable of being lixiviated was recorded around the 7th day of immersion (20); this could indicate that in this period, complete lixiviation of the free monomers does not occur (19).…”

Distribution and Excretion of BisGMA in Guinea Pigs

“…Direct evidence of BisGMA release from composite resins and fissure sealants into the biophase was provided previously (Tanaka et al, 1991;Spahl et al, 1998;Nalcaci et al, 2006). Released dental material components can be expected to enter the body by 2 different routes after resin placement, via the saliva and gastrointestinal tract, plus (if the material is placed on dentin) via the dentin and pulp (Hume and Gerzina, 1996).…”