Time-course full profiling of circulating miRNAs in neurologically deceased organ donors: a proof of concept study to understand the onset of the cytokine storm

Clément, Andrée-Anne; Lamarche, Daphnée; Masse, Marie-Hélène; Légaré, Cécilia; Tai, Lee-Hwa; Deland, Laurence Fleury; Battista, Marie-Claude; Bouchard, Luigi; D’Aragon, Frédérick

doi:10.1080/15592294.2022.2076048

Cited by 1 publication

(1 citation statement)

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“…2 In the context of organ donation, their correlation with the evolution of the cytokine storm emphasizes their potential significance in monitoring and understanding the underlying processes associated with this inflammatory response. 3 The majority of micro-RNAs are present in bodily fluids at extremely low concentrations, typically ranging from attomolar to femtomolar levels. This poses a challenge in detecting and accurately quantifying them.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance imaging signal amplification for the detection of micro-RNAs in the context of organ donation

Beltrami,

Moreau,

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et al. 2024

Biophotonics in Point-of-Care III

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Numerous medical conditions require extensive monitoring of diverse biomarkers, imposing a significant time and effort burden on healthcare professionals. This underscores the need for fast, reliable, and personalized point-of-care diagnostic tools. In the context of organ transplantation, the physiological status of a braindeceased organ donor requires comprehensive surveillance, accompanied by appropriate medical interventions to mitigate organ degradation. An effective strategy for monitoring this degradation involves tracking the progression and, ideally, the onset of the inflammatory response, the so-called "cytokine storm", by monitoring specific biomarkers: micro-RNAs. We propose two approaches based on a surface plasmon resonance imaging biosensor that combine various methodologies to amplify the hybridization signal of an oligonucleotide duplex (sandwich assay or bimaterial nanostructured biochip). In a proof-of-concept experiment involving a proteinoligonucleotide complex in a sandwich assay, we successfully demonstrate signal amplification by one order of magnitude. Additionally, using the contribution of localized surface plasmons induced by nanostructuring the biochip, our simulations predict a further significant gain in amplification.

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Section: Introductionmentioning

confidence: 99%