Time course gene expression in the one-carbon metabolism network using HepG2 cell line grown in folate-deficient medium

Chango, Abalo; Nour, Afif M. Abdel; Bousserouel, Souad; Eveillard, Damien; Anton, Pauline M.; Guéant, Jean‐Louis

doi:10.1016/j.jnutbio.2008.04.004

Cited by 19 publications

(14 citation statements)

References 36 publications

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“…Recently, it has been shown that folate deficiency in HepG2 cells produces time-dependent transcriptional changes that involve clusters of genes related to the remethylation and transmethylation pathways and transport of folates (Chango et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Placental markers of folate-related metabolism in preeclampsia

Mislanova¹,

Martsenyuk²,

Huppertz³

et al. 2011

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The etiology and degree of clinical symptoms of preeclampsia depend on genotypic and phenotypic maternal and trophoblast factors, and elevated levels of plasma homocysteine (Hcy) are one of the pathogenetic factors of preeclampsia. To assess the impact of the folate-related metabolism, we characterized the indices of this metabolism in 40 samples from uncomplicated term placentas and 28 samples from preeclamptic pregnancies by quantifying the total content of folate, methionine (Met), Hcy and related cysteine, and glutathione (GSH) in compliance with the 677 C/T genotype of methylene tetrahydrofolate reductase (MTHFR). The prevalence of MTHFR genotypes was not significantly different between the two groups. The polymorphism of MTHFR was not unambiguously connected with the content of total placental Met, Hcy and related cysteine, and GSH either in uncomplicated or in complicated pregnancies. By contrast, the combination of the heterozygous MTHFR genotype with folate deficiency in the samples from preeclamptic pregnancies was characterized by a statistically significant decrease in the Met content, a trend toward increased Hcy levels and a tight association between metabolically directly and indirectly related compounds, e.g. positive relation between Hcy versus cysteine and folate versus GSH and negative relation between folate versus Hcy and both Hcy and cysteine versus GSH. We demonstrated the expression of cystathionine-b-synthase (CBS) in human placenta at term by RT-PCR and western blot analysis, for the first time, and confirmed its catalytic activity and the accumulation of cysteine and CBS in placental explants cultivated in the presence of elevated Hcy concentrations. We suggest that disturbance in placental folate-related metabolism may be one of the pathogenetic factors in preeclampsia.

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Section: Discussionmentioning

confidence: 99%

Placental markers of folate-related metabolism in preeclampsia

Mislanova¹,

Martsenyuk²,

Huppertz³

et al. 2011

Reproduction

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“…Recent evidence suggests that alterations in gene expression specific to folate deficiency (441,442) and proteomic changes in response to folic acid supplementation (443) can be identified, which potentially could be used to supplement interpretation of established biomarkers. Furthermore, gene expression network analysis was used to identify gene expression patterns associated with micronuclei formation under different environmental exposure conditions such as acrylamide or nitrosamine exposure (444,445).…”

Section: Research Gaps and Needsmentioning

confidence: 99%

Biomarkers of Nutrition for Development—Folate Review

Lynch¹,

Pfeiffer

Georgieff

et al. 2015

The Journal of Nutrition

651

412

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The Biomarkers of Nutrition for Development (BOND) project is designed to provide evidence-based advice to anyone with an interest in the role of nutrition in health. Specifically, the BOND program provides state-of-the-art information and service with regard to selection, use, and interpretation of biomarkers of nutrient exposure, status, function, and effect. To accomplish this objective, expert panels are recruited to evaluate the literature and to draft comprehensive reports on the current state of the art with regard to specific nutrient biology and available biomarkers for assessing nutrients in body tissues at the individual and population level. Phase I of the BOND project includes the evaluation of biomarkers for 6 nutrients: iodine, iron, zinc, folate, vitamin A, and vitamin B-12. This review represents the second in the series of reviews and covers all relevant aspects of folate biology and biomarkers. The article is organized to provide the reader with a full appreciation of folate's history as a public health issue, its biology, and an overview of available biomarkers (serum folate, RBC folate, and plasma homocysteine concentrations) and their interpretation across a range of clinical and population-based uses. The article also includes a list of priority research needs for advancing the area of folate biomarkers related to nutritional health status and development.

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“…The apparent lack of biochemical regulation of the folate pathway heightens interest in mechanisms operating at the gene level, either during development or in response to folates. The latter possibility has various precedents in other organisms, including folate regulation of folate-related genes in animal cells (Chango et al, 2009;Hayashi et al, 2007), and yeast (Gelling et al, 2004). There is so far only fragmentary evidence for developmental or folate-mediated regulation of folate synthesis genes in plants.…”

Section: Introductionmentioning

confidence: 99%

Developmental and Feedforward Control of the Expression of Folate Biosynthesis Genes in Tomato Fruit

et al. 2010

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Little is known about how plants regulate their folate content, including whether the expression of folate biosynthesis genes is orchestrated during development or modulated by folate levels. Nor is much known about how folate levels impact the expression of other genes. These points were addressed using wild-type tomato fruit and fruit engineered for high folate content. In wild-type fruit, the expression of genes specifying early steps in folate biosynthesis declined during development but that of other genes did not. In engineered fruit overexpressing foreign GTP cyclohydrolase I and aminodeoxychorismate synthase genes, the expression of the respective endogenous genes did not change, but that of three downstream pathway genes-aminodeoxychorismate lyase, dihydroneopterin aldolase, and mitochondrial folylpolyglutamate synthase-respectively increased by up to 7.8-, 2.8-, and 1.7-fold, apparently in response to the build-up of specific folate pathway metabolites. These results indicate that, in fruit, certain folate pathway genes are developmentally regulated and that certain others are subject to feedforward control by pathway intermediates. Microarray analysis showed that only 14 other transcripts (of 11 000 surveyed) increased in abundance by two-fold or more in high-folate fruit, demonstrating that the induction of folate pathway genes is relatively specific.

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Time course gene expression in the one-carbon metabolism network using HepG2 cell line grown in folate-deficient medium

Cited by 19 publications

References 36 publications

Placental markers of folate-related metabolism in preeclampsia

Placental markers of folate-related metabolism in preeclampsia

Biomarkers of Nutrition for Development—Folate Review

Developmental and Feedforward Control of the Expression of Folate Biosynthesis Genes in Tomato Fruit

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