Time Course of C-Reactive Protein and Inflammatory Mediators after Neonatal Surgery

Nguyen-Vermillion, Annie; Juul, Sandra E.; McPherson, Ronald J.; Ledbetter, Daniel J.

doi:10.1016/j.jpeds.2010.12.048

Cited by 26 publications

(23 citation statements)

References 27 publications

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“…A recent study in surgical patients, including gastroschisis, showed that C-reactive protein (CRP), produced by the liver, is an extremely sensitive indicator of inflammation. In addition, corticosteroids treatment did not affect the production of CRP, enabling to quantify them due to the level of inflammation and not for direct intervention in its expression 13 .…”

Section: Resultsmentioning

confidence: 99%

“…The CRP expression can take longer to normalize due to chronic inflammation, which was does not happen with other inflammatory mediators such as IL-1b, IL-6, IL-8, IL-10 and TNF, that doesn't change their levels, and therefore are not useful to monitor a postoperative period, except for that IL-6 increases during 24 hours after a surgical procedure, but returns to its basal levels afterwards 13 .…”

Section: Resultsmentioning

confidence: 99%

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Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Gonçalves

Bittencourt²,

Velloso

et al. 2013

Acta Cir. Bras.

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Paulo (FMRP-USP). Ribeirao Preto -SP, Brazil. Mentor, conception and design of study, manuscript writing and critical revision. ABSTRACT PURPOSE:To evaluate the effect of corticosteroids on intestinal and liver interleukin profile in an experimental model of gastroschisis in fetal rats. METHODS: Sprague-Dawley rats at 19.5 days of gestation had its fetuses operated for the creation of gastroschisis. Two groups of fetuses were studied with and without maternal administration of dexamethasone. Each group was composed of fetuses who underwent gastroschisis (G), control fetuses without manipulation (C) and sham fetuses (S). A dosage of the following interleukins was carried out in fetal intestinal and liver tissues: IL-1, IL-6, IL-10, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ). The differences between the groups and subgroups were tested by ANOVA with Tukey post-test, with significant values of p<0.05. RESULTS: Dexamethasone led to an increase in intestinal and liver IL-6 (p<0.05) and a decrease in intestinal TNF-α (p<0.001) in fetuses with gastroschisis. CONCLUSION: Corticosteroids had an effect on the intestinal interleukin profile and a small effect on the liver interleukin profile due to immunological immaturity of the fetus, and also of fetuses with gastroschisis. The steroid action may not be exclusively antiinflammatory, but also pro-inflammatory, varying with time of pregnancy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Gonçalves

Bittencourt²,

Velloso

et al. 2013

Acta Cir. Bras.

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“…If the VEGF response in neonates is similar to that of adults, an operative procedure prior to 32 weeks gestation could result in sustained upregulation of VEGF during a developmental phase when infants normally would have downregulation of VEGF [13]. Surgical exposure or the infant's underlying pathology may increase inflammatory cytokines that could increase VEGF and affect vessel growth as well [17][18][19]. Disturbed VEGF regulation might contribute to more disorganized vessel formation and ultimately the need for more laser intervention.…”

Section: Discussionmentioning

confidence: 99%

Assessing the Surgical Experience to Identify Risk Factors Associated with Severe Retinopathy of Prematurity and Laser Intervention

Torr

Yoder²,

Beachy

2018

Neonatology

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Background: Retinopathy of prematurity (ROP) is a known complication of preterm birth and one of the leading causes of blindness. Known risk factors include low gestational age (GA), birth weight, and oxygen exposure. It is unknown if there are surgical risk factors associated with severe ROP. Objectives: Identify risk factors of a neonatal surgical exposure associated with laser therapy for ROP. Methods: Institutional review of 76 infants with GA ≤27 weeks or birth weight ≤800 g. Infant demographics and details of surgical experience were collected. Infants who underwent a surgical procedure were analyzed to identify risk factors associated with laser treatment for severe ROP. Surgical and nonsurgical infants were compared to assess if the rate of laser intervention differed among institutions/published data. Data were analyzed using the Mann-Whitney U test, unpaired t test, OR and Fischer’s exact test. Result: Out of 49 surgical infants, 11 underwent laser intervention. Infants undergoing laser had surgery at an earlier postmenstrual age (PMA; 31.5 [29.3–39.4] weeks vs. 38.1 [31.3–42.5] weeks, p = 0.01), were more likely to have an exploratory laparotomy (adjusted odds ratio 1.3 [1.04–1.64], p = 0.02), and to undergo more surgical procedures (3 vs. 2, p = 0.04). Conclusion: At our institution, preterm infants who undergo a surgical procedure prior to 32 weeks PMA and those having ≥3 surgeries were at a higher risk for progressing to laser intervention. Additional studies with larger sample sizes are needed to assess if the risk factors we have identified remain significant and to identify other possible risk factors.

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“…It plays an important role in host defense by binding to phosphocholine and related microbial molecules. As an early indicator of infectious or inflammatory conditions 31,32 , CRP is usually elevated in patients with neonatal sepsis [33][34][35] , meningitis, pancreatitis, pneumonia and pelvic inflammatory disease and occult bacteremia. The significantly elevated serum CRP levels are associated with malignant diseases, bacterial infections and correlate with increased 30-day mortality rates in hospitalized patients 36 .…”

Section: Introductionmentioning

confidence: 99%

“…The American Heart Association/Center for Disease Control has considered CRP as the best inflammatory marker for clinical diagnosis 37 . The precise and rapid determination of human C-reactive protein (CRP) is essential for diagnosis and management of neonatal sepsis [33][34][35]38,39 , cardiovascular diseases [40][41][42][43][44] , infectious/inflammatory conditions 31 and diabetes [45][46][47] . The ability for repeated CRP measurements with high precision in an acute setting provides clinicians with the valuable information to assess disease diagnosis and circumvent any unnecessary administration of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

A rapid one-step kinetics-based immunoassay procedure for the highly-sensitive detection of C-reactive protein

Vashist¹,

Czilwik²,

Oordt³

et al. 2014

Protocol Exchange

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A rapid one-step kinetics-based sandwich enzyme-linked immunosorbent (ELISA) procedure has been developed for highly-sensitive detection of C-reactive protein (CRP) in less than 30 min. With minimal process steps, the procedure is highly simplified and cost-effective. The analysis only involves sequentially the formation of a sandwich immune complex on capture anti-CRP antibody (Ab)-bound Dynabeads, followed by two magnet-assisted washings and an enzymatic reaction. The developed immunoassay (IA) detected CRP in the dynamic range of 0.3-81 ng mL-1 with a limit of detection and analytical sensitivity of 0.4 ng mL-1 and 0.7 ng mL-1. Its analytical precision for analysis of CRP spiked in diluted human serum, whole blood, and ethylenediaminetetraacetic acid (EDTA) plasma samples of patients was validated by conventional ELISA, unravelling its immense potential for in vitro diagnostics (IVD).

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Time Course of C-Reactive Protein and Inflammatory Mediators after Neonatal Surgery

Cited by 26 publications

References 27 publications

Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model

Assessing the Surgical Experience to Identify Risk Factors Associated with Severe Retinopathy of Prematurity and Laser Intervention

A rapid one-step kinetics-based immunoassay procedure for the highly-sensitive detection of C-reactive protein

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