Time Course of Recovery of Juvenile Skeletal Muscle After Botulinum Toxin A Injection

Ma, Jun; Elsaidi, Gamal A.; Smith, Thomas L.; Walker, Francis O.; Tan, Kay Sin; Martin, Eileen; Koman, L. Andrew; Smith, Beth Paterson

doi:10.1097/01.phm.0000137315.17214.93

Cited by 72 publications

(84 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Doses administered in this study fall within clinical recommendations and were previously demonstrated to be effective in the mouse, rat, and rabbit. 8,12,19,20 The results demonstrate a dosedependent response to BoNTA injection and that injection volume plays a critical role in determining the efficacy of a particular dose.…”

Section: Discussionmentioning

confidence: 86%

“…19 Although histology may confirm the morphometric changes in the atrophied muscle and its neuromuscular junctions, alterations in neuromuscular junction remodeling, and muscle recovery are already well documented. 1,5,12,13,15,16,19,34,35 This study explores functional outcomes after varying injection dose and volume, and recognizes that it is limited to MFG testing, EMG testing, and muscle mass.…”

Section: Discussionmentioning

confidence: 99%

“…EMG examination was conducted according to our previously published methods with the following modifications. 12 The nerve was stimulated with a nonrecurring stimulus (0.8 mA, 0.1 ms duration). EMG recording was performed twice per leg, and the average latency and compound muscle action potential (CMAP) was reported.…”

Section: Botulinum Toxin Injectionmentioning

confidence: 99%

“…The effects of BoNTA injection may be seen within 8 h but the blockade is temporary. 1,[11][12][13] Nerve growth factors, myonuclear addition, axonal sprouting, and motor end plate activity are increased within a week. [14][15][16][17] Muscle recover occurs within 3-6 months in humans which enables BoNTA to be a valuable tool in reversibly altering muscle tone.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Dose‐ and volume dependent‐response to intramuscular injection of botulinum neurotoxin‐A optimizes muscle force decrement in mice

Stone

Callahan

et al. 2011

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

Botulinum neurotoxin-A (BoNTA) is a potent neurotoxin used to alter muscle tone to manage spasticity and to provide tendon bioprotection; however, the appropriate dose and injection volume to administer is not defined. Male mice (n ¼ 120) received BoNTA injections into one gastrocnemius with either a constant volume (10 ml) with a variable dose (1, 3, 6 U/kg) or a constant dose (3 U/kg) in a variable volume (2.5, 5, 10, 20, 30 ml). Electromyographic (EMG) examination, muscle force generation (MFG), and wet muscle mass were measured in the ipsilateral and contralateral limbs at 1, 2, 4, or 12 weeks post-injection. MFG and EMG responses decreased to approximately 40% of contralateral after a 1 U/kg injection and 0% of contralateral by 3 and 6 U/kg injection at 1 week after injection. Neuromuscular blockade was greatest with a 10 ml injection volume. MFG, EMG examination, and wet muscle mass reached contralateral values 12 weeks after injection for all injection doses and volumes tested. Effective injection doses and volumes were identified for producing full and partial neuromuscular blockade in the mouse gastrocnemius. These findings have important clinical implications in the intramuscular administration of BoNTA to manage muscle tone. ß

show abstract

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Botulinum Toxin Injectionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Dose‐ and volume dependent‐response to intramuscular injection of botulinum neurotoxin‐A optimizes muscle force decrement in mice

Stone

Callahan

et al. 2011

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…For a limb skeletal muscle, the lateral gastrocnemius was selected because it is composed largely of fast muscle fiber types (Armstrong and Phelps, 1984), which is similar to the high percent of fast muscle fiber types found in extraocular muscle. The lateral gastrocnemius muscle can be easily stimulated in situ with wire electrodes in contact with the lateral tibial nerve and has been used frequently for in situ contractile studies (Ameredes and Provenzano, 1997;Grassi et al, 2002;de Haan, 1998;Ma et al, 2004).…”

Section: Surgical Preparation Of Animalsmentioning

confidence: 99%

Measurement of contractile force of skeletal and extraocular muscles: Effects of blood supply, muscle size and in situ or in vitro preparation

Croes

Bartheld

2007

Journal of Neuroscience Methods

View full text Add to dashboard Cite

Contractile forces can be measured in situ and in vitro. To maintain metabolic viability with sufficient diffusion of oxygen, established guidelines for in vitro skeletal muscle preparations recommend use of relatively thin muscles (≤1.25 mm thick). Nevertheless, forces of thin extraocular muscles vary substantially between studies. Here, we examined parameters that affect force measurements of in situ and in vitro preparations, including blood supply, nerve stimulation, direct muscle stimulation, muscle size, oxygenated or non-oxygenated buffer solutions, and the time after interruption of vascular circulation. We found that the absolute forces of extraocular muscle are substantially lower when examined in vitro. In vitro preparation of 0.58 mm thick extraocular muscle from 3-week old birds underestimated contractile function, but not of thinner (0.33 mm) muscle from 2-day old birds. Our study shows that the effective criteria for functional viability, tested in vitro, differ between extraocular and other skeletal muscle. We conclude that contractile force of extraocular muscles will be underestimated by between 10 and 80%, when measurements are made after cessation of blood supply (at 5 -40 min). The mechanisms responsible for the declining values for force measurements are discussed, and we make specific recommendations for obtaining valid measurements of contractile force.

show abstract

Botulinum Toxin (Clostridium Botulinum)

Pilch¹,

Bray²

2011

Encyclopedia of Bioterrorism Defense

View full text Add to dashboard Cite

Botulinum toxin is classified as a Category A agent of bioterrorism because of the ability of an extremely small dose to interrupt cholinergic nerve transmission, leading to paralysis and death. Treatment is currently limited to the carefully supervised administration of equine antitoxin after early symptom onset, an approach that would be inadequate in the event of a massive botulism outbreak. Efforts to develop new therapies applicable to a mass‐casualty situation focus on blocking specific steps in pathogenesis and speeding the recovery of neuronal and muscular function. This entry first reviews basic features of botulinum toxin, sources of poisoning, the clinical syndrome, current approaches to diagnosis and management, and the potential of the toxin as a bioterrorist weapon. It then presents the steps in the pathogenesis and recovery from botulism at the molecular level, accompanied by a description of current research efforts aimed at developing specific therapeutic measures.

show abstract

Time Course of Recovery of Juvenile Skeletal Muscle After Botulinum Toxin A Injection

Abstract: Our findings provide scientific evidence to support the clinical situation in which the interinjection interval of 3-6 mos of botulinum toxin A at a similar dosage is used.

Cited by 72 publications

References 32 publications

Dose‐ and volume dependent‐response to intramuscular injection of botulinum neurotoxin‐A optimizes muscle force decrement in mice

Dose‐ and volume dependent‐response to intramuscular injection of botulinum neurotoxin‐A optimizes muscle force decrement in mice

Measurement of contractile force of skeletal and extraocular muscles: Effects of blood supply, muscle size and in situ or in vitro preparation

Botulinum Toxin (Clostridium Botulinum)

Contact Info

Product

Resources

About