Time course of the age-related alterations in stored blood

Türkes, Sibel; Korkmaz, Özden; Korkmaz, Mustafa

doi:10.1016/s0301-4622(03)00141-8

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…Oxidation of sulfhydryls of the membrane protein to disulfides causes reversible changes. This may be due to the disulfide exchange reactions carried out by a class of thioltransferases that catalyze reactions between glutathione and thioredoxin to regenerate the protein sulfhydryls [ 48 ]. These may be the possible reasons for variations in sulfhydryls during the storage.…”

Section: Discussionmentioning

confidence: 99%

Prospects of Vitamin C as an Additive in Plasma of Stored Blood

Vani

Ravikumar

Hsieh

et al. 2015

Advances in Hematology

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There is a dire necessity to improve blood storage and prolong shelf-life of blood. Very few studies have focused on oxidative stress (OS) in blood and its influence on plasma with storage. This study attempts to (i) elucidate the continuous changes occurring in plasma during storage through oxidant levels and antioxidant status and (ii) evaluate the influence of vitamin C (VC) as an additive during blood storage. Blood was drawn from male Wistar rats and stored for 25 days at 4°C. Blood samples were divided into control and experimental groups. Plasma was isolated every 5 days and the OS markers, antioxidant enzymes, lipid peroxidation, and protein oxidation products, were studied. Catalase activity increased in all groups with storage. Lipid peroxidation decreased in VC (10) but was maintained in VC (30) and VC (60). Although there were variations in all groups, carbonyls were maintained towards the end of storage. Advanced oxidation protein products (AOPP) increased in VC (30) and were maintained in VC (10) and VC (60). Sulfhydryls were maintained in all groups. Vitamin C could not sufficiently attenuate OS and hence, this opens the possibilities for further studies on vitamin C in combination with other antioxidants, in storage solutions.

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Section: Discussionmentioning

confidence: 99%

Prospects of Vitamin C as an Additive in Plasma of Stored Blood

Vani

Ravikumar

Hsieh

et al. 2015

Advances in Hematology

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“…[5] used ESR to detect human hemoglobin from bloodstains. Türkes et al [6] analyzed blood stored under blood bank conditions using ESR. They reported that the intensity of ESR signals from methemoglobin, nonheme irons, and organic radicals in dried human blood increase with time.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

Ito

Mori

Kanazawa

et al. 2011

Journal of Toxicology

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Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗ and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗ in liver (y = 113.8–40.7 log (day), R1 = −0.779, R2 = 0.6, P < .001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗ signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

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“…Currently, a large amount of research effort is focused on developing hemoglobin (Hb) based oxygen carriers to address various issues associated with blood transfusions. − One of the most compelling reasons for developing oxygen carriers that can replace lost volumes of blood is the impending shortage of donor blood, especially in cases of natural disasters and wars. − Additionally, the costs associated with blood collection, storage, and distribution are steadily increasing. ,− An oxygen carrier suitable for human use would help alleviate the risks associated with blood transfusions, which include transmittance of infectious diseases, mild allergic reactions, delayed hemolytic transfusion reactions, acute respiratory distress syndrome, and fatal acute hemolytic transfusion reactions. −,− Furthermore, the use of donated human blood is limited due to loss of efficacy associated with deterioration of the red blood cell (RBC) as evidenced by changes in oxygen affinity, solution pH, RBC hemolysis, RBC deformability, microaggregation, and protein denaturation compared to fresh blood. , …”

Section: Introductionmentioning

confidence: 99%

Physical Properties of Hemoglobin−Poly(acrylamide) Hydrogel-Based Oxygen Carriers: Effect of Reaction pH

Patton

Palmer

2006

Langmuir

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This work examines the physical properties of bovine hemoglobin (BHb) chemically cross-linked to a pH responsive polymer (poly(acrylamide)) with the goal of taking advantage of the polymer's pH sensitivity to generate low-P50 oxygen carriers for application in physiological conditions characterized by deviations from normal pH. BHb-hydrogel-based oxygen carriers encapsulating 10-16 g/dL BHb displayed P50s < 10 mmHg when encapsulated inside of liposomes (i.e. Hb-LGs) and <15 mmHg in the absence of a lipid bilayer (i.e. Hb-NHPs), when synthesized at pHs less than normal physiological pH. The results of this work suggest that synthesis of Hb-LGs/Hb-NHPs under different pH conditions affect the ionization of BHb and the flexibility of the polymer chains to which BHb is chemically cross-linked. The degree of BHb cross-linking to the polymer matrix and the chain flexibility of the polymer influences the oxygen affinity and cooperativity of the oxygen carrier by influencing how easy it is for the Hb molecule to change conformations between the R and T states. The magnitude of the zeta potential of Hb-LGs and Hb-NHPs was shown to be within the range of stored red blood cells and within the range of limited flocculation. Taken together, this work describes the preparation and characterization of oxygen carriers with increased oxygen affinities compared to those of red blood cells.

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Time course of the age-related alterations in stored blood

Cited by 5 publications

References 24 publications

Prospects of Vitamin C as an Additive in Plasma of Stored Blood

Prospects of Vitamin C as an Additive in Plasma of Stored Blood

Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

Physical Properties of Hemoglobin−Poly(acrylamide) Hydrogel-Based Oxygen Carriers: Effect of Reaction pH

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