Time-course of the retinal nerve fibre layer degeneration after complete intra-orbital optic nerve transection or crush: A comparative study

Parrilla–Reverter, G.; Agudo, Marta; Nadal-Nicolás, Francisco M.; Alarcón-Martínez, Luis; Jiménez-López, Manuel; Salinas‐Navarro, Manuel; Sobrado‐Calvo, Paloma; Bernal-Garro, José Manuel; Villegas‐Pérez, María Paz; Vidal‐Sanz, Manuel

doi:10.1016/j.visres.2009.08.020

Cited by 64 publications

(63 citation statements)

References 60 publications

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“…1988; Parrilla‐Reverter et al. 2009) or ocular hypertension (Salinas‐Navarro et al. 2009, 2010; Vidal‐Sanz et al.…”

Section: Resultsmentioning

confidence: 99%

“…2014) or RGCs (Brn3a) and their intraretinal axons (pNFH, Parrilla‐Reverter et al. 2009) were double‐immunodetected. Primary antibodies were rabbit anti‐Iba‐1 (1:500 dilution; Dako; Rafer, Zaragoza, Spain), mouse IgG1 anti‐pNFH (1:200 dilution, Clone RT‐97; Serotec, Bionova, Spain) and goat anti‐Brn3a (1:750 dilution; C‐20, Santa Cruz Biotechnology, Heidelberg, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…1988; Parrilla‐Reverter et al. 2009). Secondary detection was carried out with Alexa Fluor‐conjugated antibodies (donkey anti‐rabbit Alexa 594, donkey anti‐goat Alexa 488 and goat anti‐mouse IgG1 Alexa 488, all diluted at 1:500; Molecular Probes ThermoFisher, Madrid, Spain).…”

Section: Methodsmentioning

confidence: 99%

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MicroRNA regulation in an animal model of acute ocular hypertension

Wang

Valiente‐Soriano

Nadal-Nicolás

et al. 2016

Acta Ophthalmologica

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PurposeTo analyse miRNA regulation in a rat model of acute ocular hypertension (AOH).MethodsAcute ocular hypertension (AOH) was induced in the left eye of adult albino rats by inserting a cannula connected with a saline container into the anterior chamber. The contralateral eye served as a control. Seven days later, animals were killed. Retinas were used either for quantitative analysis of retinal ganglion cells (RGCs) and microglial cells or for miRNA array hybridization, qRT‐PCR and Western blotting.ResultsAnatomically, AOH caused axonal degeneration, a significant loss of RGCs and a significant increase in microglial cells in the ganglion cell layer. The miRNAs microarray analysis revealed 31 differentially expressed miRNAs in the AOH versus control group, and the regulation of 12 selected microRNAs was further confirmed by qRT‐PCR. Bioinformatic analysis indicates that several signalling pathways are putatively regulated by the validated miRNAs. Of particular interest was the inflammatory pathway signalled by mitogen‐activated protein kinases (MAPKs). In agreement with the in silico analysis, p38 MAP kinase, tumour necrosis factor‐alpha (TNF‐α) and iNOS proteins were significantly upregulated in the AOH retinas.ConclusionsAcute IOP elevation led to changes in the expression of miRNAs, whose target genes were associated with the regulation of microglia‐mediated neuroinflammation or neural apoptosis. Addressing miRNAs in the process of retinal ischaemia and optic nerve damage in association with high IOP elevation may open new avenues in preventing retinal ganglion cell apoptosis and may serve as target for future therapeutic regimen in acute ocular hypertension and retinal ischaemic conditions.

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“…1988; Parrilla‐Reverter et al. 2009) or ocular hypertension (Salinas‐Navarro et al. 2009, 2010; Vidal‐Sanz et al.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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MicroRNA regulation in an animal model of acute ocular hypertension

Wang

Valiente‐Soriano

Nadal-Nicolás

et al. 2016

Acta Ophthalmologica

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“…Thus, as a model to study the underlying causes of RGC death by axonal injury, IONC is clean and predictable. Traumatic axonal injury to the optic nerve, either by complete transection or crush, is a well established model that can be evaluated morphologically Parrilla-Reverter et al, 2009a, molecularly (Agudo et al, 2008) and functionally, and has been thoroughly analyzed by our group, both in rats and mice (Alarcon-Martinez et al, 2009, 2010. These lesions induce a quick and massive death of RGCs (Peinado-Ramon et al, 1996;Chidlow et al, 2005;Sobrado-Calvo et al, 2007;Agudo et al, 2008Galindo-Romero et al, 2011;Sanchez-Migallon et al, 2011).…”

Section: Intraorbital Optic Nerve Crush (Ionc)mentioning

confidence: 99%

Anatomical and Molecular Responses Triggered in the Retina by Axonal Injury

Agudo‐Barriuso¹,

Nadal-Nicolás²,

Parrilla–Reverter³

et al. 2011

The Mystery of Glaucoma

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“…Those methods are reversible and have been shown to be valuable in understanding the role of patterned vision and low-level correlation of binocular inputs in sculpting cortical circuits during development [6][7][8] . In glaucoma research, the optic nerve crush model in adult animals has been widely used because it establishes a progressive loss of retinal ganglion cell inputs that constitute the optic nerve 9,10 . On the other hand, enucleation, where the eye and thus the retina is completely and instantly removed, is the appropriate choice of deprivation when the aim is to irreversibly remove both spontaneous and patterned vision at once.…”

Section: Introductionmentioning

confidence: 99%

A Highly Reproducible and Straightforward Method to Perform <em>In Vivo</em> Ocular Enucleation in the Mouse after Eye Opening

Aerts

Nys

Arckens

2014

JoVE

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Enucleation or the surgical removal of an eye can generally be considered as a model for nerve deafferentation. It provides a valuable tool to study the different aspects of visual, cross-modal and developmental plasticity along the mammalian visual system [1][2][3][4] .Here, we demonstrate an elegant and straightforward technique for the removal of one or both eyes in the mouse, which is validated in mice of 20 days old up to adults. Briefly, a disinfected curved forceps is used to clamp the optic nerve behind the eye. Subsequently, circular movements are performed to constrict the optic nerve and remove the eyeball. The advantages of this technique are high reproducibility, minimal to no bleeding, rapid post-operative recovery and a very low learning threshold for the experimenter. Hence, a large amount of animals can be manipulated and processed with minimal amount of effort. The nature of the technique may induce slight damage to the retina during the procedure. This side effect makes this method less suitable as compared to Mahajan et al. (2011) 5 if the goal is to collect and analyze retinal tissue. Also, our method is limited to post-eye opening ages (mouse: P10 -13 onwards) since the eyeball needs to be displaced from the socket without removing the eyelids. The in vivo enucleation technique described in this manuscript has recently been successfully applied with minor modifications in rats and appears useful to study the afferent visual pathway of rodents in general.

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Time-course of the retinal nerve fibre layer degeneration after complete intra-orbital optic nerve transection or crush: A comparative study

Cited by 64 publications

References 60 publications

MicroRNA regulation in an animal model of acute ocular hypertension

MicroRNA regulation in an animal model of acute ocular hypertension

Anatomical and Molecular Responses Triggered in the Retina by Axonal Injury

A Highly Reproducible and Straightforward Method to Perform <em>In Vivo</em> Ocular Enucleation in the Mouse after Eye Opening

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