Time-course study of the accumulation of hydroxyproline-rich glycoproteins in root cells of susceptible and resistant tomato plants infected by Fusarium oxysporum f. sp. radicis-lycopersici

Abstract

The accumulation of hydroxyproline-rich glycoproteins (HRGPs) in cell walls of dicotyledonous plants is thought to be involved in the defense response to pathogens. An antiserum raised against deglycosylated HRGPs from melon was used for studying the subcellular localization of these glycoproteins in susceptible and resistant tomato (Lycopersicon esculentum Mill.) root tissues infected by Fusarium oxysporum f.sp. radicis-lycopersici. A time-course of HRGP accumulation revealed that these glycoproteins increased earlier and to a higher extent in resistant than in susceptible cultivars. In the compatible interaction, increase in HRGPs was largely correlated with pathogen invasion and appeared to occur as a result of wall damage. In the incompatible interaction, HRGPs accumulated in the walls of uninvaded cells, thus indicating a possible role in the protection against fungal penetration. The occurrence of substantial amounts of HRGPs in papillae, known to be physical barriers formed in response to infection, and in intercellular spaces provides additional support to the concept that such glycoproteins play an important role in disease resistance.

Infection induced oxidative cross-linking of hydroxyproline-rich glycoproteins (HRGPs) is associated with restriction ofColletotrichum sublineolumin sorghum

“…These results confirm and extend several previous studies which have reported more rapid increases in cell wall HRGPs in resistant than in susceptible genotypes in different host-pathogen interactions such as the cucumber Á Cladosporium cucumerinum (Hammerschmidt et al 1984), the tomato Á Fusarium oxysporum f. sp. radicis-lycopersici (Benhamou et al 1991), the tobacco Á Erysiphe cichoracearum (Raggi 2000), the wheat Á F. culmorum (Kang and Buchenauer 2003) and the pearl millet Á Sclerospora graminicola (Shailasree et al 2004) interactions. HRGPs were extracted from cell walls by a harsher treatment using a combination of acid and ethanol.…”

Hrp Mutant of Pseudomonas syringae pv phaseolicola Induces Cell Wall Alterations but Not Membrane Damage Leading to the Hypersensitive Reaction in Lettuce

“…Benhamou et al (1991) consider that the major epitopes recognized by the HRGP 2b antiserum are on the polypeptide moiety of the molecule. The accumulation of HRGPs within deposits and cell walls prior to the detection of phenolic compounds at these sites is consistent with their proposed role in providing nucleation sites for lignin-like polymer deposition (Whitmore, 1978).…”

Induction Patterns of an Extensin Gene in Tobacco upon Nematode Infection.

“…Localized induction of extensin proteins has been documented in several compatible and incompatible plant-fungi (Benhamou et al, 1991) or plant-bacteria (Mazau and Esquerre-Tugaye, 1986;O'Connell et al, 1990) interactions, both in regions of contact with the pathogen and in the neighboring intercellular space. We thus expected to observe a similar phenomenon upon nematode infection.…”

“…Whereas significant efforts have been devoted to studying HRGPs in plant-fungus (Benhamou et al, 1990a(Benhamou et al, , 1991, plantbacteria (OConnell et al, 1990), and plant-virus (Benhamou et al, 1990b) interactions, to our knowledge, no work has been reported on HRGPs in plant-nematode interactions.…”