Time-Dependent Effect of Cyclosporine on Mitogenic Responses and Lymphocyte Subset Populations in Rat Spleen and Submaxillary Lymph Nodes

Esquifino, Ana I.; Arce, Agustı́n; Selgas, Laura; Cardinali, Daniel P.

doi:10.1159/000026424

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…The relative size distributions of lymph cells in submaxillary lymph nodes of rats were determined by fluorescence‐activated cell sorter analysis, as previously described ( 13, 14). For these studies, we used the following monoclonal antibodies: Anti‐rat LCA (OX‐33) for B lymphocytes (Serotec, Oxford, UK), anti‐rat TCR alpha/beta (R7.3) for T lymphocytes (Serotec, Oxford, UK), anti‐rat CD4 (OX‐35) which recognizes a rat T helper cell differentiation antigen (Pharmingen, San Diego, CA, USA), and anti‐rat CD8a (OX‐8) which recognize the reactive antigen expressed on rat T cytotoxic/suppressor cells (Pharmingen).…”

Section: Methodsmentioning

confidence: 99%

Effect of Melatonin Treatment on 24‐h Variations in Responses to Mitogens and Lymphocyte Subset Populations in Rat Submaxillary Lymph Nodes

Castrillón

Esquifino

Varas

et al. 2000

J Neuroendocrinology

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Wistar male rats were injected s.c. with melatonin (30 microg) or vehicle, 1 h before lights off, for 11 days. Ten days after beginning melatonin treatment, rats received Freund's complete adjuvant or its vehicle s.c., and after 2 days, they were sacrificed at six different time intervals throughout a 24-h cycle. The mitogenic effect of lipopolysaccharide (LPS) and concanavalin A (Con A), the activity of ornithine decarboxylase (ODC) and the relative size of lymphocyte subset populations were measured in submaxillary lymph nodes. In control rats, the mitogenic effects of LPS and Con A and ODC activity peaked during the afternoon. Injection of Freund's adjuvant induced a 10-h shift in the diurnal rhythm of the mitogenic effect of LPS to attain maximal values at night. Melatonin pretreatment blunted the daily variations in the mitogenic activity of Con A or LPS and, when given to Freund's adjuvant-injected rats, augmented mesor and amplitude of diurnal rhythm in ODC activity. Maxima in B cell number occurred at night whereas those of T and B-T cell number occurred during the afternoon. During the early phase of immunization tested, the number of B cells augmented and the amplitude of its diurnal rhythmicity increased both after immunization and following melatonin pretreatment. Maxima of 24-h rhythms in CD4+ and CD4+/CD8+ cell populations occurred during the afternoon while those of CD8+ cells occurred at late night. Melatonin significantly augmented CD4+ cell number and decreased CD8+ cell number; it therefore augmented the CD4+:CD8+ ratio. The results suggest that pretreatment with a pharmacological dose of melatonin exerts immunomodulating effects at an early, preclinical, phase of Freund's adjuvant-induced arthritis in rats.

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Section: Methodsmentioning

confidence: 99%

Effect of Melatonin Treatment on 24‐h Variations in Responses to Mitogens and Lymphocyte Subset Populations in Rat Submaxillary Lymph Nodes

Castrillón

Esquifino

Varas

et al. 2000

J Neuroendocrinology

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“…The subsets (T lymphocyte, B lymphocyte and NK cell) of lymphocytes were determined by a direct immunofluorescent staining with a flow cytometric analysis ( 35 ). We used the Rat T/B/NK Cell Cocktail (Fluorescence-labeled antibody cocktail, Becton Dickinson, USA): APC-conjugated CD3 for T lymphocyte (clone 1F4); fluoresce in isothiocyanate (FITC)-conjugated anti-rat CD45RA for B lymphocyte (clone OX-33); and Phycoerythrin (PE)-conjugated anti-rat CD161a for NK cells (clone 10/78).…”

Section: Methodsmentioning

confidence: 99%

Zinc-Deficiency Induced Changes in the Distribution of Rat White Blood Cells

Someya

Tanihata

Sato

et al. 2009

J Nutr Sci Vitaminol

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Summary Zinc is known to play an important role for immune-functions. However, the effects of zinc-deficiency on the immune response system from the point of view of the distribution changes of the number of total white blood cells (WBCs) are still primarily unknown. Therefore, the effects of zinc-deficiency on the number of total WBCs, neutrophil, eosinophil, basophil, monocyte and lymphocytes (T lymphocyte, B lymphocyte and NK cell) were studied in rats. The weaned male rats were randomly divided into the zinc deficient diet (ZDD: 0.7 mg zinc/kg diet) group and the control diet (CON: 34.8 mg zinc/kg diet) group, and were pair-fed for 4 wk. The number of lymphocyte subsets, visceral organ weights, serum zinc, corticosterone and IL-6 concentrations were also determined. Zinc-deficiency increased duration-dependently the number of total white blood cells, granulocytes (neutrophil, eosinophil and basophil) and monocytes in 2-4 wk without changing the number of lymphocytes, T lymphocytes, B lymphocytes or NK cells. The relative weights of thymus and adrenals were 0.63 times ( p Ͻ 0.01) lower and 1.60 times ( p Ͻ 0.001) higher in ZDD group than in CON group, respectively. Zinc-deficiency increased serum corticosterone concentration to 1.48 times ( p Ͻ 0.05) without changing serum IL-6 concentration, as compared with those of CON group. From these results, zinc-deficiency increases markedly the number of granulocytes and monocytes without changing the number of lymphocytes, T lymphocytes, B lymphocytes or NK cells. These results also suggest that zinc-deficiency induces stress responses and the responses may have in part participated in increased actions of the number of granulocytes and monocytes during zinc-deficiency, and induce thymus atrophy and adrenal hypertrophy.

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“…Splenocyte proliferation was evaluated after in vitro stimulation with Concanavalin A (ConA5μg/mL), Phytohaemagglutinin A (PHA) and Lypopolysaccharide (LPS 3 μg/mL). These three mitogens are commonly reported to display cell proliferation [31][32][33][34]. The CFSE fluorescence intensity is inversely correlated with cell proliferation.…”

Section: Cell Proliferation Evaluationmentioning

confidence: 98%

Effects of different doses and schedules of diazepam treatment on lymphocyte parameters in rats

Lima

Sakai

Latorre

et al. 2010

International Immunopharmacology

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Time-Dependent Effect of Cyclosporine on Mitogenic Responses and Lymphocyte Subset Populations in Rat Spleen and Submaxillary Lymph Nodes

Cited by 7 publications

References 20 publications

Effect of Melatonin Treatment on 24‐h Variations in Responses to Mitogens and Lymphocyte Subset Populations in Rat Submaxillary Lymph Nodes

Effect of Melatonin Treatment on 24‐h Variations in Responses to Mitogens and Lymphocyte Subset Populations in Rat Submaxillary Lymph Nodes

Zinc-Deficiency Induced Changes in the Distribution of Rat White Blood Cells

Effects of different doses and schedules of diazepam treatment on lymphocyte parameters in rats

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