Time-Dependent Effects of a High-Energy-Yielding Diet on the Regulation of Specific White Adipose Tissue Genes

Abstract

White adipose tissue development is regulated by many factors, including the energy content of food and the genetic background. Nevertheless, little is known about possible differential effects of high-fat palatable diets when fed for short or long-time periods. Thus, the expression of certain genes involved with lipid metabolism (peroxisome proliferator-activated receptor gamma, PPAR␥2; retinoic receptors; fatty acid binding protein, aP2 and uncoupling proteins, UCP) may be affected by those dietary manipulations (highenergy-yielding diet and time duration of feeding). High-fat feeding for 8 days decreased mRNA UCP3 levels compared to control fed animals, while feeding for 30 days increased them over controls. Similar findings occurred for PPAR␥2 and aP2. Furthermore, statistically significant associations were found among PPAR␥2, aP2 and UCP3 mRNA levels. These data suggest a physiological time-dependent response seeking to prevent excessive fat deposition when animals are fed for short-term with a high amount of dietary fat, which was followed by an adaptive period to the highenergy content of diet throughout a coregulation among certain lipid metabolism related genes: PPAR␥2, aP2, UCP3.

Anti-obesity Effects of Juniperus chinensis Extract Are Associated with Increased AMP-Activated Protein Kinase Expression and Phosphorylation in the Visceral Adipose Tissue of Rats

“…44) In addition, because aP2 facilitates intracellular mobilization of free fatty acids, 45,46) and free fatty acids are known to be natural PPARg2 agonists, a possible association among PPARg2, UCP3 and aP2 in the white adipose tissue has been suggested. 47) Supporting these reports, the results of this study indicate that the expression levels of PPARg2, aP2, UCP2 and UCP3 genes are positively correlated, and that these four genes are regulated towards the same direction by the experimental diets in the visceral fat tissue. Epididymal mRNA levels of these genes decrease in response to an HFD (vs. an ND) and increase in response to an HFD+JCE (vs. an HFD).…”

Down-regulation of heart HFABP and UCP2 gene expression in diet-induced (cafeteria) obese rats

“…Similarly, MARGARETa et al (9) found also a statistically significant correlation between FABP (ap2), UCP3 and PPARy mRNA levels in white adipose tissue from control and obese animals.…”

Time‐dependent regulation of muscle caveolin activation and insulin signalling in response to high‐fat diet

“…Subsequently, at day 35, a delayed response characterized by the establishment of an obese metabolism was observed [24], with the animals showing hyperglycemia, hyperinsulinemia and insulin resistance. In accordance to this, Cav-1 and IR are no longer activated.…”