Time-dependent effects of chitosan on dentin structures

Silva, Polliana Vilaça; Guedes, Débora Fernandes Costa; Pécora, Jesus Djalma; Filho, Antônio Miranda da Cruz

doi:10.1590/s0103-64402012000400008

Cited by 87 publications

(131 citation statements)

References 22 publications

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“…Pimentaet al [44] reported that the 0.2% chitosan solution (pH=3.2) has a capacity to reduce dentin microhardness similar to that of 15% EDTA (pH=7.25) representing similar demineralization potential. The effect of 0.2% chitosan applied for 3 minutes was the most effective for removing the smear layer and smear plug with minimal erosive effect on dentin substrate as suggested previously in the study of Silva et al [38].…”

Section: Discussionsupporting

confidence: 66%

“…The most reported causes of their failure are retention loss or marginal degradation [39]. Many factors were proposed to be involved in bond degradation and different methods were used to reproduce clinical situations and simulate the oral environment in order to test the dentin bonding durability [37,38]. Among these methods, thermal cycling, load cycling and prolonged water or artificial saliva incubation were commonly used [39,40].…”

Section: Discussionmentioning

confidence: 99%

“…Group CH: Dentin surfaces were conditioned with cotton pellets soaked in 0.2% chitosan solution for three minutes [37,38] (pH=3.2, Fig. 1a).…”

Section: Dentin Conditioningmentioning

confidence: 99%

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Adhesive strength of self-adhesive resins to lithium disilicate ceramic and dentin: effect of dentin chelating agents

2014

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The aim of this study was to test the effect of different chelating agents on microtensile bond strength (MTBS) of self-adhesive resin cements to dentin. The occlusal surfaces of extracted human mandibular molars (N = 80) were cut horizontally to expose sound dentin. The teeth were randomly divided into four groups (n = 20 per group) and dentin surfaces were conditioned according to one of the following methods: group C: no treatment (control group); group CH: 0.2 % chitosan; group E: 17 % ethylene diamine tetra acetic acid (EDTA) and group P: 25 % polyacrylic acid (PAA). Lithium disilicate glass ceramic (e.max CAD) blocks were cemented to conditioned dentin surfaces with self-adhesive cements (RelyX Unicem or Clearfil SA) and photo-polymerized. Specimens were stored in distilled water at 37°C for 24 h and thermocycled for 6,000 times. The beams were obtained from bonded ceramic-cement-tooth assemblies and were subjected to the MTBS test (1 mm/min). Failure types were analyzed and selected beams were examined under scanning electron microscope. Data (MPa) were analyzed using two-way ANOVA and Tukey's test (P < 0.05). While cement type significantly affected the MTBS results (P < 0.05), no significant difference was observed between the dentin chelating agents (P = 0.785). Interaction terms were not significant (P = 0.114). Control group with no dentin conditioning presented significantly lower results with both cements (RelyX Unicem: 8.1 ± 1.9(a), Clearfil SA: 8 ± 1.6(a)) than those of conditioned groups (19.3 ± 4.2(b)-24.5 ± 5.2(b)) (P < 0.05). Failure types were predominantly adhesive in all groups. Chitosan (2 %), EDTA (17 %) or PAA (25 %) could all be used as dentin chelating agents in conjunction with self-adhesive resin cements tested. Abstract:The aim of this study was to test the effect of different chelating agents on microtensile bond strength (MTBS) of self-adhesive resin cements to dentin. The occlusal surfaces of extracted human mandibular molars (N=80) were cut horizontally to expose sound dentin. The teeth were randomly divided into 4 groups (n=20 per group) and dentin surfaces were conditioned according to one of the following methods: Group C: No treatment (Control group); Group CH: 0.2% chitosan; Group E: 17% ethylene diamine tetra acetic acid (EDTA) and Group P: 25% polyacrylic acid (PAA). Lithium disilicate glass ceramic (e.max CAD) blocks were cemented to conditioned dentin surfaces with self-adhesive cements (RelyX Unicem or Clearfil SA) and photo-polymerized.Specimens were stored in distilled water at 37 o C for 24 hours and thermocycled for 6000 times.The beams were obtained from bonded ceramic-cement-tooth assemblies and they were subjected to the MTBS test (1 mm/min). Failure types were analyzed and selected beams were examined under scanning electron microscope. Data (MPa) were analyzed using two-way ANOVA and Tukey's test (P<0.05). While cement type significantly affected the MTBS results (P<0.05), no significant difference was observed between the dentin chelating agents (...

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

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Adhesive strength of self-adhesive resins to lithium disilicate ceramic and dentin: effect of dentin chelating agents

2014

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“…However, endodontic irrigants may alter the physicochemical properties of dentin (3,9). NaOCl causes oxidation of the organic matrix and denaturation of collagen, changing the chemical structure of dentin and affecting its mechanical properties (10).…”

Section: Introductionmentioning

confidence: 99%

Effect of sodium hypochlorite and edta irrigation, individually and in alternation, on dentin microhardness at the furcation area of mandibular molars

2012

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The aim of this study was to evaluate the effect of irrigation regimens on dentin microhardness at the furcation area of mandibular molars, using sodium hypochlorite and ethylenediaminetetraacetic acid (EDTA), individually and in alternation. The occlusal surface and the roots of 20 non-carious extracted human permanent mandibular molars were cut transversally and discarded. The tooth blocks were embedded in acrylic resin and randomly assigned to 4 groups (n=5) according to the irrigating regimens: 1% NaOCl solution, 17% EDTA solution, 1% NaOCl and 17% EDTA and distilled water (control). Knoop microhardness of dentin at the furcation area was evaluated. Data were analyzed using one-way ANOVA and Tukey's multiple comparison tests (α=0.05). The results of this study indicated that all irrigation solutions, except for distilled water (control), decreased dentin microhardness. EDTA did not show a significant difference with NaOCl/EDTA (p>0.05), but showed a significant difference with NaOCl (p<0.01). EDTA and NaOCl/EDTA showed a maximum decrease in microhardness. The 17% EDTA solution, either alone or in combination with 1% NaOCl reduced significantly dentin microhardness at the furcation area of mandibular molars.

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“…In the present study, the decalcification properties of two chelators were evaluated when associated with different protocols of agitation. Chelating agents play an important role in the process of removing smear layer, as they increase dentin permeability [23, 24]. We assessed the effectiveness of two chelating substances (0.2% Chitosan and 17% EDTA) based on the amount of Calcium ions released, using atomic absorption spectrometry.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Amount of Calcium Ions Released after the use of Different Chelating Agents and Agitation Protocols

Pedro

Costa

Filho

et al. 2017

TODENTJ

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Background:The main goal of endodontic treatment is to achieve cleaning and shaping prior to the filling process.Objective:This study aimed to evaluate, using atomic absorption spectrometry, the release of Calcium ions after the use of different chelating agents and protocols of agitation.Method:Ninety human canine teeth were randomly assigned to one of nine groups (n=10), as follows: 1) 0.2% Chitosan and manual agitation; 2) 0.2% Chitosan and sonic agitation; 3) 0.2% Chitosan and ultrasonic agitation; 4) 17% EDTA and manual agitation; 5) 17% EDTA and sonic agitation; 6) 17% EDTA and ultrasonic agitation; 7) distilled water and manual agitation; 8) distilled water and sonic agitation; 9) distilled water and ultrasonic agitation. Following instrumentation, all chelating substances remained inside the root canal for 3 min. Then the fluid was collected for the identification and quantification of Calcium ions. The amount of Calcium ions released in each group was compared using analysis of variance (ANOVA) and the Kolmogorov-Smirnov and Levene tests followed by Tukey’s post-hoc test. Significance was set at 5%.Results:The groups in which 0.2% Chitosan was used showed the highest concentration of Calcium ions (p<0.05). Concerning the agitation method, ultrasonic agitation showed the greatest values, followed by sonic and manual agitation (all comparisons, p<0.05).Conclusion:The present findings suggest that, among the combinations here tested, Chitosan associated with ultrasonic agitation yielded the greatest release of Calcium ions.

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Time-dependent effects of chitosan on dentin structures

Cited by 87 publications

References 22 publications

Adhesive strength of self-adhesive resins to lithium disilicate ceramic and dentin: effect of dentin chelating agents

Adhesive strength of self-adhesive resins to lithium disilicate ceramic and dentin: effect of dentin chelating agents

Effect of sodium hypochlorite and edta irrigation, individually and in alternation, on dentin microhardness at the furcation area of mandibular molars

Assessment of the Amount of Calcium Ions Released after the use of Different Chelating Agents and Agitation Protocols

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