Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues

Banday, Anees Ahmad; Farooq, Neelam; Priyamvada, Shubha; Yusufi, A. N. K.; Khan, Farah

doi:10.1016/j.lfs.2007.11.014

Cited by 109 publications

(63 citation statements)

References 34 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Association of GM-induced nephrotoxicity with marked increase in levels of Cr and BUN has been reported, 7,26 and we observed signiˆcant increases of both Cr and BUN levels in the GM group compared with the control group (Fig. 8).…”

Section: Pº0supporting

confidence: 73%

“…2,4 Although the exact mechanism of GM-induced nephrotoxicity is unclear, ROS are considered important mediators. 2,3,[5][6][7][8][9][10][11] Recent studies have shown molecular hydrogen as an e‹cient antioxidant, owing to its ability to diŠuse rapidly into tissues and cells across membranes. 12 It has also been reported that water dissolving molecular hydrogen (hydrogen-rich water [HW]) improved metamorphosis accompanying decreased apoptosis in the kidney and nephrotoxicity induced by cisplatin, a widely used drug for cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protective Effect of Hydrogen-rich Water against Gentamicin-induced Nephrotoxicity in Rats using Blood Oxygenation Level-dependent MR Imaging

Matsushita

Kusakabe

Kitamura

et al. 2011

MRMS

View full text Add to dashboard Cite

Purpose: We assessed intrarenal oxygenation in gentamicin-induced nephrotoxicity (GIN) and the protective eŠect of hydrogen-rich water (HW) against GIN using blood oxygenation level-dependent magnetic resonance (MR) imaging.Materials and Methods: We acquired T 2 * -weighted images (T 2 * WI) of 21 rats on Days 0, 2, 4, and 7 using a 1.5-tesla MR imaging system. The rats were divided into 3 groups of seven each: control rats had free access to standard water and no gentamicin (GM) injection; rats designated the GM group had free access to standard water and were injected with GM (80 mg/kg/day) subcutaneously for 7 days; and the third group, designated the GM＋HW group, had free access to HW and were injected with GM. R 2 * (＝1/T 2 *) was estimated from T 2 *WI.Results: R 2 * values in the cortex were signiˆcantly decreased on Days 2, 4, and 7 compared with those on Day 0 in the GM group but not signiˆcantly changed in the control and GM＋HW groups. R 2 * values in the medulla did not change signiˆcantly in any group.Conclusions: Ourˆndings suggested reduced oxygen utility, mainly in the cortex, in gentamicin-induced nephrotoxicity and an ameliorative eŠect of hydrogen-rich water against GIN.

show abstract

Section: Pº0supporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Protective Effect of Hydrogen-rich Water against Gentamicin-induced Nephrotoxicity in Rats using Blood Oxygenation Level-dependent MR Imaging

Matsushita

Kusakabe

Kitamura

et al. 2011

MRMS

View full text Add to dashboard Cite

show abstract

“…Since brush border membrane (BBM) and other intracellular organelles such as mitochondria and lysosomes are known GEN target, 38,39 the structural/functional integrity was assessed by the status of their respective biomarker enzymes. So, here, we measured the MDA, GSH, and NO, as a means of oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Pomegranate Extract Attenuates Gentamicin-Induced Nephrotoxicity in Rats by Reducing Oxidative Stress

et al. 2012

View full text Add to dashboard Cite

Nephrotoxicity is a major complication of gentamicin (GEN), which is widely used in the treatment of severe Gramnegative infections. Reactive oxygen species are important mediators of GEN-induced nephrotoxicity. Because of the strong antioxidant properties of pomegranate extract (PE), we evaluated the protective effect of PE against GEN-induced nephrotoxicity. Thirty-two adult male rats were randomly divided into four equal groups: (1) controls; (2) treated with GEN for 14 consecutive days (100 mg/kg/day); (3) treated with GEN plus distilled water; and (4) treated with GEN plus PE (100 μL). After 15 days, the rats were killed and their kidneys were taken, and blood analysis was performed. Tubular necrosis and interstitial fibrosis scores were determined histopathologically; and biochemically, nitric oxide (NO), malondialdehyde (MDA), and reduced glutathione (GSH) levels in kidneys were determined. Urea, creatinine, Na þ , and K þ levels were investigated in the blood analysis. Statistical analyses were made by the chi-square test and analysis of variance. Serum urea and creatinine levels were significantly higher in rats treated with GEN alone than rats in the control and the GEN þ PE-treated groups. The GSH level in renal tissue of only GEN-treated rats was significantly lower than those in the control group, and administration of PE to GEN-treated rats significantly increased the level of GSH. The group that was given GEN and PE had significantly lower MDA levels in kidney cortex tissue than those given GEN alone. There was no significant difference of NO levels between the groups. In rats treated with GEN þ PE, despite the presence of mild tubular degeneration and tubular necrosis is less severe, and glomeruli maintained a better morphology when compared with the GEN-treated group. We think that PE prevents kidney damage by decreasing oxidative stress in kidney.

show abstract

“…Chez le rongeur, suite à une exposition aiguë, à raison de 0,5 à 5 mg/kg en injection intrapéritonéale unique, une variation au niveau rénal de l'expression des enzymes antioxydantes (SOD, catalase…) et une augmentation de la peroxydation lipidique sont observées (Banday et al, 2008a ;Belles et al, 2007 ;Priyamvada et al, 2010). Chez le rat, après exposition chronique (3 mois, 40 mg/kg) l'uranium induit une augmentation de marqueurs du stress oxydant (peroxydation lipidique et oxydation du glutathion (GSSG)) au niveau rénal (Linares et al, 2006).…”

Section: Stress Oxydantunclassified

Données nouvelles sur la néphrotoxicité de l’uranium

Guéguen

Rouas

2012

Radioprotection

View full text Add to dashboard Cite

RÉSUMÉL'uranium est un radioélément ainsi qu'un métal lourd auquel l'homme peut être exposé du fait de sa présence dans l'environnement ou des activités humaines. Il exerce principalement une toxicité chimique au niveau du tubule contourné proximal du rein après exposition aiguë ou chronique. Après exposition à une forte dose d'uranium, la néphrite tubulaire aiguë est révélée par une protéinurie ainsi qu'une diminution du débit de filtration glomérulaire. Plus récemment, l'utilisation de marqueurs d'intégrité tissulaire telle que la béta 2-microglobuline ou des enzymes tissulaires a pu être corrélée aux altérations histopathologiques des tubules contournés proximaux. Lors d'exposition chronique à de faible niveau, l'utilisation de marqueurs plus spécifiques et plus sensibles s'avère nécessaire lorsque les lésions sont faibles. Des études expérimentales développées au laboratoire ont montré la pertinence de certains de ces biomarqueurs tel que Kim-1 pour détecter de faibles altérations rénales. L'uranium exerce sa cytotoxicité au niveau des cellules épithéliales des tubules contournés proximaux probablement du fait de son entrée dans la cellule où il s'accumule préférentiellement dans le noyau cellulaire, lorsqu'il n'est pas sous forme de précipités. Les mécanismes conduisant à la mort des cellules ne sont pas encore totalement élucidés mais le stress oxydant semble y jouer un rôle important. ABSTRACT New data on uranium nephrotoxicity.Uranium is an element and also a heavy metal to which humans can be exposed due to its natural presence or human activities. It has mainly a chemical toxicity on the proximal convoluted tubules of the kidney after acute or chronic exposure. At high dose exposure, acute tubular nephritis is observed, as indicated by proteinuria and a decreased glomerular filtration rate. More recently, the use of structural biomarkers such as beta 2-microglobuline or tubular enzymes has been correlated with histopathological injury of the proximal convoluted tubules. During chronic exposure at a low level, the use of some of these sensitive and specific biomarkers would be necessary, particularly when the injury is slight. Recent experimental studies in the laboratory have shown the relevance of such new biomarkers as Kim-1 to detect slight renal injury. Uranium exerts its toxicity on epithelial tubular cells in which it accumulates mainly in the nucleus when it is not precipitated. The mechanisms leading to cell death are not totally elucidated but oxidative stress seems to play an important role.

show abstract

Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues

Cited by 109 publications

References 34 publications

Protective Effect of Hydrogen-rich Water against Gentamicin-induced Nephrotoxicity in Rats using Blood Oxygenation Level-dependent MR Imaging

Protective Effect of Hydrogen-rich Water against Gentamicin-induced Nephrotoxicity in Rats using Blood Oxygenation Level-dependent MR Imaging

Pomegranate Extract Attenuates Gentamicin-Induced Nephrotoxicity in Rats by Reducing Oxidative Stress

Données nouvelles sur la néphrotoxicité de l’uranium

Contact Info

Product

Resources

About