Time-dependent radiation characteristics of Nannochloropsis oculata during batch culture

Heng, Ri-Liang; Pilon, Laurent

doi:10.1016/j.jqsrt.2014.04.008

Cited by 27 publications

(30 citation statements)

References 29 publications

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“…Figure 2(b) demonstrates that the scattering cross section hC sca;λ i of the cell grown under nitrogen-limited conditions was larger than that of the cell grown under nitrogen-replete conditions. This was also consistent with experimental measurements [5]. It can be attributed to (i) the reduction in absorption by pigments and (ii) the increase in volume fraction of metabolites featuring larger refractive index n 6;λ than other intracellular compartments (Tables 1 and 2).…”

Section: A Radiation Characteristics Of Microalgae Cell Grown Under supporting

confidence: 91%

“…Figure 2(a) also shows that the spectral absorption cross section hC abs;λ i of the cell grown under nitrogenlimited conditions was smaller than that of the cell grown under nitrogen-replete conditions and decreased with time due to the decrease in pigment concentrations associated with the increase in lipid content ( Table 2). These observations are consistent with experimental measurements for N. oculata [5] and Anabaena cylindrica [44]. Moreover, the predictions of the spectral absorption cross section hC abs;λ i by the volume-equivalent homogeneous sphere and the coated sphere approximations were in excellent agreement (within 5%) with those by the superposition T-matrix method for all growth conditions considered.…”

Section: A Radiation Characteristics Of Microalgae Cell Grown Under supporting

confidence: 88%

“…(1). In addition, the order of magnitude of hC abs;λ i predicted was similar to that measured experimentally for N. oculata 1.5-3.5 μm in diameter [5,6]. Figure 2(a) also shows that the spectral absorption cross section hC abs;λ i of the cell grown under nitrogenlimited conditions was smaller than that of the cell grown under nitrogen-replete conditions and decreased with time due to the decrease in pigment concentrations associated with the increase in lipid content ( Table 2).…”

Section: A Radiation Characteristics Of Microalgae Cell Grown Under supporting

confidence: 84%

“…On the other hand, Table 2 summarizes the range of radius r 6 , number of spheres N 6 , and the total volume V 6 of lipid compartments, as well as the Chl a, chlorophyll b (Chl b), and photoprotective carotenoid (PPC) concentrations in the simulated microalgae grown under nitrogen-replete and at two different times after sudden nitrogen starvation, namely 12 and 24 h. Indeed, when subjected to nitrogen starvation, C. reinhardtii and other microalgae (e.g., N. oculata) reduce their pigment concentration Chl a, Chl b, and PPC concentrations and simultaneously increase their lipid content [5,6,24,28,33]. Here, each pigment concentration was reduced, from their values under nitrogen-replete conditions, by the same proportions as those observed experimentally for N. oculata [6].…”

Section: A Problem Statementmentioning

confidence: 99%

“…Estimating the absorption and scattering cross sections, the scattering phase function and the backscattering ratio of photosynthetic eukaryotic microalgae cells have been a subject of interest in various fields, including ocean optics for real-time monitoring of algal blooms using satellite remote sensing [1][2][3], biofuel production [4][5][6], and biomass production of value-added products [7]. In remote sensing of microalgae blooms, the objective is to monitor and detect the blooms by comparing the spatial and temporal changes in chlorophyll a (Chl a) concentration maps [8].…”

Section: Introductionmentioning

confidence: 99%

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Can spherical eukaryotic microalgae cells be treated as optically homogeneous?

Bhowmik

Pilon

2016

J. Opt. Soc. Am. A

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This study aims to answer the question of whether spherical unicellular photoautotrophic eukaryotic microalgae cells, consisting of various intracellular compartments with their respective optical properties, can be modeled as homogeneous spheres with some effective complex index of refraction. The spectral radiation characteristics in the photosynthetically active region of a spherical heterogeneous microalgae cell, representative of Chlamydomonas reinhardtii and consisting of spherical compartments corresponding to the cell wall, cytoplasm, chloroplast, nucleus, and mitochondria, were estimated using the superposition T-matrix method. The effects of the presence of intracellular lipids and/or starch accumulation caused by stresses, such as nitrogen limitation, were explored. Predictions by the T-matrix method were qualitatively and quantitatively consistent with experimental measurements for various microalgae species. The volume-equivalent homogeneous sphere approximation with volume-averaged effective complex index of refraction gave accurate estimates of the spectral (i) absorption and (ii) scattering cross sections of the heterogeneous cells under both nitrogen-replete and nitrogen-limited conditions. In addition, the effect of a strongly refracting cell wall, representative of Chlorella vulgaris, was investigated. In this case, for the purpose of predicting their integral radiation characteristics, the microalgae should be represented as a coated sphere with a coating corresponding to the cell wall and a homogeneous core with volume-averaged complex index of refraction for the rest of the cell. However, both homogeneous sphere and coated sphere approximations predicted strong resonances in the scattering phase function and spectral backscattering cross section that were not observed in that of the heterogeneous cells.

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Section: A Radiation Characteristics Of Microalgae Cell Grown Under supporting

confidence: 91%

Section: A Radiation Characteristics Of Microalgae Cell Grown Under supporting

confidence: 88%

Section: A Radiation Characteristics Of Microalgae Cell Grown Under supporting

confidence: 84%