Time Dependent Release of Interleukin-8 and Tumor Necrosis Factor-α in Platelet Concentrate

Abstract

Contaminating white blood cells in stored platelet concentrate (PC) are the source of many pro-inflammatory cytokines. These are implicated in transfusion reactions. To study the release of interleukin (IL)-8 and tumor necrosis factor alpha (TNF-a) at different time interval in PC prepared byplatelet rich plasma (PRP) and buffy coat (BC) using different principles. Fifteen PCs were prepared by both the methods. The supernatants of PCs prepared by PRP and BC methods were collected aseptically after 1, 18, 65 and 112 h of preparation. pH, platelet and WBC counts were done. The supernatants were frozen in aliquots at -56°C for measurement of IL-8 and TNF-a concentration using ELISA. The Mean ± SD value of WBC in PRP-PC was 7.4 ± 3.75 9 10 7 and in BC-PC 3.9 ± 2.2 9 10 7

The differences in the levels of interleukin 1 beta (IL-1ß) and tumor necrosis factor alpha (TNF-Α) based on the storage of thrombocyte concentrates

“…Shukla et al, found a relationship between the number of leucocytes and the TNF-α concentration (Shaiegan, 2006). The variation of TNF-α production in this study is due to the different number of leucocytes left in the TC component, but in this study, the number of leucocytes per bag was not measured (Shukla et al, 2014).…”