Time does matter! Acute copper exposure abolishes rhythmicity of clock gene in Danio rerio

Doria, Halina Binde; Bóia-Ferreira, Marianna; Rodrigues, Silvia; Lo, Sze Mei; Domingues, Cinthia Eloise; Nakao, Lia S.; Campos, Sandro Xavier de; Ribeiro, Ciro Alberto de Oliveira; Randi, Marco Antônio Ferreira

doi:10.1016/j.ecoenv.2018.02.068

Cited by 18 publications

(5 citation statements)

References 79 publications

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“…While the animal-type CRY2 (zCRY2) and zCRY4 have only weak or no transcriptional repression ability for the CLOCK:BMAL complex, the four animal-type CRY1s have strong transcriptional repression abilities 14 , so may not only be involved in the circadian clock but also in clock-associated functions, such as the photoperiodic response. Although daily zCry expression patterns have been investigated both in vivo [2][3][4][5][6]8,11 and in vitro 7,9,10 , we comparatively reinvestigated the spatiotemporal expression profiles and light-responsiveness of zCrys to test the above hypothesis. We found that zCry1ab expression in the eye changes depending on the day length.…”

Section: Continuedmentioning

confidence: 99%

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

Okano

Saratani

Tamasawa

et al. 2020

Sci Rep

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The zebrafish (Danio rerio) is a model species that is used to study the circadian clock. it possesses light-entrainable circadian clocks in both central and peripheral tissues, and its core circadian factor cryptochromes (CRYs) have diverged significantly during evolution. In order to elucidate the functional diversity and involvement of cRYs in photoperiodic mechanisms, we investigated the daily expression profiles of six Cry transcripts in central (brain and eye) and peripheral (fin, skin and muscle) tissues. the zCry genes exhibited gene-specific diurnal conserved variations, and were divided into morning and evening groups. notably, zCry1ab exhibited biphasic expression profiles in the eye, with peaks in the morning and evening. comparing ocular zCry1ab expression in different photoperiods (18L:6D, 14L:10D, 10L:14D and 6L:18D) revealed that zCry1ab expression duration changed depending on the photoperiod: it increased at midnight and peaked before lights off. zCry1ab expression in constant light or dark after entrainment under long-or short-day conditions suggested that the evening clock and photic input pathway are involved in photoperiod-dependent zCry1ab expression. Laser microdissection followed by qRt-pcR analysis showed that the evening peak of zCry1ab was likely ascribed to visual photoreceptors. These results suggest the presence of an eye-specific photoperiodic time measurement served by zCry1ab.The circadian clock consists of three parts: the input pathway(s), the oscillation system and the output pathway(s). Light is the most important signal that activates the input pathway to synchronize the oscillator with daily external cycles. The oscillator generates time signals to control physiological circadian functions through the output pathway. Internal time signals are integrated with the environmental light signal to measure day length, which triggers seasonal photoperiodic responses in many organisms living in temperate zones 1 .The zebrafish (Danio rerio) circadian clock is one of the most studied among fish species, and is reported to exist in not only the central tissues but also the embryo, larva, peripheral tissues and cultured cells (refs. 2-11 in Table 1 , refs. 12-15 ). These zebrafish circadian clocks are directly light-entrainable and this feature is also seen in the other fish cells 16 in contrast to the mammalian circadian clocks that are not light-entrainable except for those in the eye.Among vertebrate core clock components such as period (PER), CLOCK, BMAL and cryptochrome (CRY) proteins, CRYs have both a wide evolutionary background and divergent molecular functions 6 . CRYs are structurally classified into several groups, and together form a large protein family with the photo repair enzymes, photolyases (PHRs). Among CRY/PHR family proteins, animal-type CRYs (CRY1 and CRY2) play a central role in the circadian clock oscillator. Animal-type CRY1 and CRY2 function as transcriptional repressors in the core loop of the circadian clock oscillation system 17,18 , while fruit fly CRY (...

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Section: Continuedmentioning

confidence: 99%

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

Okano

Saratani

Tamasawa

et al. 2020

Sci Rep

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“…Circadian rhythms have an essential role in sleep cycles, hormone secretion, blood pressure, and other routine life processes. Zebrafish is a good model organism for circadian rhythms studies (Doria et al 2018 ; Yang et al 2019 ). The circadian rhythms are endogenously driven, controlled by genes.…”

Section: Discussionmentioning

confidence: 99%

Assessment of eco-toxic effects of commonly used water disinfectant on zebrafish (Danio rerio) swimming behaviour and recovery responses: an early-warning biomarker approach

Ren

Ramesh

et al. 2022

Environ Sci Pollut Res

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Eco-toxicity profiles for commonly used disinfectants were lacking. Available traditional toxicity techniques have some limitations (assessments and ethical issues). Behaviour toxicology is a promising research area towards early warning and non-invasive approaches. We studied the potential eco-toxic effects of sodium hypochlorite (NaOCl) on the swimming behaviour of zebrafish. Zebrafish were exposed to different concentrations (Treatment I, Treatment II, Treatment III, and Treatment IV) of NaOCl for 360 h. Recovery study (144 h) was conducted for NaOCl treatment groups. The swimming behaviour of zebrafish was quantified efficiently using an online monitoring system (OMS). OMS dataset was processed for determination of behavioural differences by MATLAB and SPSS. Compared to the control group, the swimming strength of zebrafish under NaOCl treatments declined significantly ( p < 0.001). Avoidance behaviour has occurred on zebrafish under NaOCl exposure periods. Furthermore, NaOCl toxicity also adjusted circadian rhythms on zebrafish. Zebrafish swimming strength was significantly ( p < 0.001) improved under-recovery periods. Moreover, normal diurnal patterns have occurred. NaOCl could cause behavioural abnormalities in non-target organisms. Continuous exposure to common disinfectants could cause external and internal stress on non-target organisms, resulting in behavioural changes and circadian rhythm adjustments. Continuous changes in behavioural and circadian rhythms might reduce organisms’ fitness and adaptation capacity. This study highlights (1) the importance of computer-based toxicity assessments, and (2) swimming behaviour is an early warning biomarker for eco-toxicity studies. Graphic Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s11356-021-18333-1.

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“…Interestingly, visual and light perception upon exposure to Cd were some overrepresented biological functions found on our results that are still hardly discussed in the literature. These pathways might be related either with the role of Cd on circadian disruption through oxidative stress and immune response (Jiménez-Ortega et al, 2011; Xiao et al, 2016), and therefore having an altered perception of the environment (Doria et al, 2018). As well as the differences on Cd damage extent between the dark and the light phase (Xiao et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Detecting Cd adaptation footprint in C. riparius with a multi-genomic approach

Doria

Hannapel

Pfenninger

2021

Preprint

Self Cite

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Evolutionary processes and acquired tolerance to toxicants are important factors governing how animals respond to chemical exposure. Evidence for increased tolerance to cadmium (Cd), a widely distributed toxic metal in aquatic environments, in Chironomus riparius is conflicting and still questioned if it happens through phenotypic plasticity or genetic adaptation. The present study considered the relevance of directional environmental changes by increasing contaminant concentration in a multigenerational selection experiment. Evaluation of measurable life-cycle traits, transcriptomic responses and quantitative genetics from an evolve and resequencing (E&R) experiment were integrated to assess the potential of C. riparius to adapt to Cd. Survival tests revealed some adaptation to Cd exposure. Genomic analyses showed a strong, genome-wide selective response in all replicates, emphasizing that even control laboratory conditions continually exert selective pressure. The integration of transcriptomic and genomic data could isolate the genes related to Cd acquired resistance. Those genes could be linked to an efflux of metals. Therefore, it is possible to conclude that C. riparius can endure long-term Cd exposure also through genetic adaptation.

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Time does matter! Acute copper exposure abolishes rhythmicity of clock gene in Danio rerio

Cited by 18 publications

References 79 publications

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

Assessment of eco-toxic effects of commonly used water disinfectant on zebrafish (Danio rerio) swimming behaviour and recovery responses: an early-warning biomarker approach

Detecting Cd adaptation footprint in C. riparius with a multi-genomic approach

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