2017
DOI: 10.1364/ao.56.005047
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Time-domain measurements reveal spatial aberrations in a sub-surface two-photon microscope

Abstract: We show that in a nonlinear microscopy system the effects of chromatic and spherical aberrations are revealed by a difference in the focal positions corresponding to the shortest pulse duration and the minimum lateral resolution. By interpreting experimental results from a high-numerical-aperture two-photon microscope using a previously reported spatio-temporal model, we conclude that the two-photon autocorrelation of the pulses at the focal plane can be used to minimize both the chromatic and spherical aberra… Show more

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Cited by 4 publications
(1 citation statement)
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“…Therefore, it offers many possibilities to visualize the internal structure of silicon ICs with high 3D spatial resolution (Ramsay et al, 2005; Ramsay, Serrels, et al, 2007). Another application of 2P OBIC using a SIL is found in the time‐domain measurements of pulse duration and two‐photon autocorrelation trace in a CMOS ICs that can be manipulated to minimize the chromatic and spherical aberrations in a nonlinear microscopy system (Rutkauskas et al, 2017). It reveals that the minimum pulse duration and maximum autocorrelation amplitude is attained by optimizing the objective lens position and the illumination wavefront, which could result in the best spatial resolution.…”
Section: Microscopic Measurements Of Obicmentioning
confidence: 99%
“…Therefore, it offers many possibilities to visualize the internal structure of silicon ICs with high 3D spatial resolution (Ramsay et al, 2005; Ramsay, Serrels, et al, 2007). Another application of 2P OBIC using a SIL is found in the time‐domain measurements of pulse duration and two‐photon autocorrelation trace in a CMOS ICs that can be manipulated to minimize the chromatic and spherical aberrations in a nonlinear microscopy system (Rutkauskas et al, 2017). It reveals that the minimum pulse duration and maximum autocorrelation amplitude is attained by optimizing the objective lens position and the illumination wavefront, which could result in the best spatial resolution.…”
Section: Microscopic Measurements Of Obicmentioning
confidence: 99%