Time-domain terahertz spectroscopy of artificial skin

Corridon, Peter R.; Ascazubi, Ricardo; Krest, Courtney; Wilke, Ingrid

doi:10.1117/12.646632

Cited by 22 publications

(15 citation statements)

References 44 publications

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“…To this end, across the years a variety of microscopic and macroscopic approaches have been used to evaluate primary scaffold constituents 14 – 16 , as well as whole kidney scaffold structure and function 9 , 10 , 13 , 17 – 20 . In several animal models, such techniques have shown that the decellularization process successfully removes native cellular components while retaining the vascular and ECM structures.…”

Section: Background and Summarymentioning

confidence: 99%

Intravital microscopy datasets examining key nephron segments of transplanted decellularized kidneys

Corridon

2022

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This study contains intravital microscopy (IVM) data examining the microarchitecture of acellular kidney scaffolds. Acellular scaffolds are cell-free collagen-based matrices derived from native organs that can be used as templates for regenerative medicine applications. This data set contains in vivo assays that evaluate the effectiveness of decellularization and how these acellular nephron compartments perform in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, and structural and functional integrities of decellularized tubular and peritubular capillary segments were acquired and compared to the native (non-transplanted) organ. Cohorts of 2–3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n = 4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). Micrographs and supporting measurements are provided to illustrate IVM processes used to perform this study and are publicly available in a data repository to assist scientific reproducibility and extend the use of this powerful imaging application to analyze other scaffold systems. Measurements(s) DNA quantification • tissue fluorescence • microvascular leakage • tubular and peritubular capillary integrity Technology Type(s) intravital microscopy • multiphoton microscopy • UV-visible spectroscopy Sample Characterization(s) rats • native and decellularized kidneys

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Section: Background and Summarymentioning

confidence: 99%

Intravital microscopy datasets examining key nephron segments of transplanted decellularized kidneys

Corridon

2022

Sci Data

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“…Collagen types I (predominant form), II, V, VI, XII, XIII, XIV, and XXIV, as well as various glycosaminoglycans can all be located in the ECM 18,20 . Keratocytes, mesenchymal-derived fibroblast cells with complex interactions with corneal epithelium, are the most abundant cell type in the corneal stroma and have various functions regarding the synthesis and organization of the ECM 21,22 . They are usually characterized by a typical dendritic morphology with cell-processes up to 50 mm long, and in some histological stains, the cells appear to be flattened and quiescent 23 .…”

Section: Introductionmentioning

confidence: 99%

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Pantić

Cumic

Valjarević

et al. 2023

Preprint

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Decellularized corneas offer a promising and sustainable source of replacement grafts, mimicking native tissue and reducing the risk of immune rejection post-transplantation. Despite great success in achieving acellular scaffolds, little consensus exists regarding the quality of the decellularized extracellular matrix (ECM). Metrics used to evaluate ECM performance are study-specific, subjective, and semi-quantitative. Thus, this work focused on developing a computational method to examine the effectiveness of corneal decellularization. We combined conventional semi-quantitative histological assessments and automated scaffold evaluations based on textual image analyses to assess decellularization efficiency. Our study highlights that it is possible to develop contemporary artificial intelligence (AI) models based on random forests and support vector machine algorithms, which can identify regions of interest in acellularized corneal stromal tissue with relatively high accuracy. These results provide a platform for developing AI biosensing systems for evaluating fine morphological changes in decellularized scaffolds crucial for evaluating their functionality.

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“…Furthermore, for decades, infrared-based systems have offered a quantitative and qualitative analysis of biological samples for various applications (27,(39)(40)(41)(42)(43)(44)(45). For our purposes, this infrared-based approach provided evidence of the effective removal of appreciable levels of residual detergent and cellular/tissue debris, via the absence of spectral peaks in the frequency range 2849 cm -1 to 3075 cm -1 (46) in the elute collected on the 2 nd and 3 rd day during the washout process (FIGURE 2c).…”

Section: Discussionmentioning

confidence: 99%

A scalable corneal xenograft platform: simultaneous opportunities for tissue engineering and circular economic sustainability by repurposing slaughterhouse waste

Wang¹,

Shakeel²,

Salih³

et al. 2023

Preprint

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High-throughput platforms that can generate copious quantities of corneal grafts will be invaluable in addressing the existing global demand for keratoplasty. Slaughterhouses generate substantial underutilized biological waste that can be repurposed to reduce current environmentally unfriendly practices. Such efforts to support ecological sustainability can simultaneously drive the development of transplantable ovine corneas and patient-specific bioartificial keratoprostheses. This study presents a dataset highlighting techniques that pioneered the development of a simplified, inexpensive, and high-throughput corneal xenograft platform. To the best of our knowledge, this is the first dataset to be generated in the United Arab Emirates devised on repurposing slaughterhouse waste in a manner that supports circular economic practices. These efforts outline the first step in developing a platform that can produce dozens of native ovine xenografts and acellular corneal scaffolds that effectively preserve ocular transparency, transmittance, and ECM components, and can be used to potentially create individualized and transplantable grafts. Such native grafts can then be genetically modified to reduce rejection and retrovirus transmission in recipients. Analogously, decellularization technologies can support corneal regeneration with key attributes comparable to native xenografts.

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Time-domain terahertz spectroscopy of artificial skin

Cited by 22 publications

References 44 publications

Intravital microscopy datasets examining key nephron segments of transplanted decellularized kidneys

Intravital microscopy datasets examining key nephron segments of transplanted decellularized kidneys

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

A scalable corneal xenograft platform: simultaneous opportunities for tissue engineering and circular economic sustainability by repurposing slaughterhouse waste

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