Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles

Hadjidemetriou, Marilena; Al-Ahmady, Zahraa S.; Kostarelos, Kostas

doi:10.1039/c5nr09158f

Cited by 179 publications

(184 citation statements)

References 33 publications

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“…1 mL of B or L was mixed with 390 µL of NPs at 4.6 mg Fe mL −1 , corresponding to 1.3 m 2 of NPs mL −1 of serum, close to previously reported values . The PC formation is a dynamic process, which is rapidly changing during the first 2–3 h, as shown in vitro and in vivo . Hence, based on these two reported studies, NPs were incubated for 24 h at 37 °C under agitation either at 1400 or 20 rpm.…”

Section: Methodssupporting

confidence: 84%

Protein Corona: Impact of Lymph Versus Blood in a Complex In Vitro Environment

Bonvin

Alexander

Chiappe

et al. 2017

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In biological environments, the surface of nanoparticles (NPs) are modified by protein corona (PC) that determines their biological behavior. Unfortunately, in vitro tests still give different PC than in vivo tests causing in vitro-in vivo discrepancy; hence, in vitro studies are not indicative for the NPs' behavior in vivo. Here is demonstrated that PC in vitro is strongly influenced by the type of extracellular fluid (ECF), blood or lymph, by their high and low flow conditions and transitions between ECFs, and a combination of these parameters. As a result, this in vitro study approaches fluidic and dynamic variations to which NPs are exposed in vivo: different ECF that NPs encounter first in different injection routes, different transitions in-between ECFs during circulation, and simultaneous change in the exposed flow in these transitions. The most-abundant proteins in PCs are found to be not the most abundant in ECFs, but those having high affinity for binding to the surface of NPs. Moreover, some proteins are differently abundant in PCs at different flows, which indicate force-promoted binding, catch bonds. These results suggest that future in vitro studies should consider more complex incubation conditions to improve the in vitro-in vivo consistency necessary for translational research.

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Section: Methodssupporting

confidence: 84%

Protein Corona: Impact of Lymph Versus Blood in a Complex In Vitro Environment

Bonvin

Alexander

Chiappe

et al. 2017

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“…In reality the dispersed particles are hydrated/ solvated which along with its corona are rarely spherical. The composition of the corona -especially the soft corona -is dynamic and fluctuates over time depending on the ionic strength, types of smaller and bigger molecules present in the environment and types of solvents [52,53]. Therefore, DLS provides only an indicative size of the colloid.…”

Section: R H (Hydrodynamic Radius) and R G (Radius Of Gyration)mentioning

confidence: 99%

DLS and zeta potential – What they are and what they are not?

Bhattacharjee

2016

Journal of Controlled Release

3,021

1,673

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“…Although relevant, factors defined by NPs are fewer compared to media-dependent factors. Composition of the NPs corona depends on the source of proteins [29–31], time [9,32] and temperature [33] of the incubation of NPs in biological fluids. Not all variables are so intuitive; corona composition can also be affected by serum heat inactivation [34], choice of anticoagulant [30], static versus dynamic conditions of the media [35] and even the presence of magnetic field in the case of iron oxide NPs [36].…”

Section: Introductionmentioning

confidence: 99%

Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona

et al. 2017

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Protein corona of nanoparticles (NPs), which forms when these particles come in to contact with protein-containing fluids, is considered as an overlooked factor in nanomedicine. Through numerous studies it has been becoming increasingly evident that it importantly dictates the interaction of NPs with their surroundings. Several factors that determine the compositions of NPs protein corona have been identified in recent years, but one has remained largely ignored—the composition of media used for dispersion of NPs. Here, we determined the effect of dispersion media on the composition of protein corona of polyacrylic acid-coated cobalt ferrite NPs (PAA NPs) and silica NPs. Our results confirmed some of the basic premises such as NPs type-dependent specificity of the protein corona. But more importantly, we demonstrated the effect of the dispersion media on the protein corona composition. The differences between constituents of the media used for dispersion of NPs, such as divalent ions and macromolecules were responsible for the differences in protein corona composition formed in the presence of fetal bovine serum (FBS). Our results suggest that the protein corona composition is a complex function of the constituents present in the media used for dispersion of NPs. Regardless of the dispersion media and FBS concentration, majority of proteins from either PAA NPs or silica NPs coronas were involved in the process of transport and hemostasis. Interestingly, corona of silica NPs contained three complement system related proteins: complement factor H, complement C3 and complement C4 while PAA NPs bound only one immune system related protein, α-2-glycoprotein. Importantly, relative abundance of complement C3 protein in corona of silica NPs was increased when NPs were dispersed in NaCl, which further implies the relevance of dispersion media used to prepare NPs.

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Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles

Cited by 179 publications

References 33 publications

Protein Corona: Impact of Lymph Versus Blood in a Complex In Vitro Environment

Protein Corona: Impact of Lymph Versus Blood in a Complex In Vitro Environment

DLS and zeta potential – What they are and what they are not?

Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona

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