Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells

Takagi, Mutsumi; Shibaki, Yuhei

doi:10.1016/j.jbiosc.2012.05.024

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…The phase shift in PC-3 cells in the interphase was markedly lower than that in PRECs throughout the cell cycle. Therefore, it was proposed that adherent PC-3 cancer cells could be noninvasively discriminated with high sensitivity and specificity from normal adherent PRECs by the periodical measurement of phase shift during culture using PLM (Takagi and Shibaki 2012). However, periodical measurement of phase shift in many cells requires a long time, and it is desired to discriminate cancer cells precisely and noninvasively by one-time measurement of the phase shift in each cell.…”

Section: Introductionmentioning

confidence: 99%

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Noninvasive discrimination between human normal and cancer cells by analysis of intracellular distribution of phase-shift data

Takagi

Tokunaga

2015

Cytotechnology

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Aiming to establish a method for the noninvasive discrimination of cancer cells from normal cells in adherent culture, we investigated to employ all phase shift data for all pixels inside a cell. The bird's-eye views of phase shifts of human prostate epithelial cells (PRECs) and human prostatic carcinoma epithelial cell (PC-3) lines acquired by phase-shifting laser microscopy showed tableland and cone shapes, respectively, while treatment of PRECs with cytochalasin D resulted in the cone shape. So, the profile of phase shift in both sections towards the x- and y-axes of the views through the peaks of the phase shifts in PRECs and PC-3 cells were trapezoid-like and triangle-like, respectively. Typical profiles of phase shifts in a section in PRECs or PC-3 cells were calculated by averaging from 10 cells and smoothing. Cancer index is defined as the deduction of sums of the squared difference between a real cell and the typical profiles for a PREC and a PC-3 cell. The cancer indices for PC-3 and hepatocellular carcinoma cell lines were positive, while those for PRECs and human normal cryopreserved hepatocytes were negative. Cancer indices along the major axis of fibroblast-like cells of normal mesenchymal stem cells and the osteosarcoma cell line were negative and positive, respectively. Consequently, several cancer cells could be noninvasively discriminated from normal cells by calculating the cancer index employing phase shift for all pixels inside the cells.

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Section: Introductionmentioning

confidence: 99%

“…Although many phase shift data for many pixels in a cell were available, only the highest phase shift in a cell was employed in those previous studies mentioned above (Tokumitsu et al 2010;Takagi and Tokunaga 2013;Takagi and Shibaki 2012).…”

Section: Introductionmentioning

confidence: 99%

Noninvasive discrimination between human normal and cancer cells by analysis of intracellular distribution of phase-shift data

Takagi

Tokunaga

2015

Cytotechnology

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Cell Processing Engineering for Regenerative Medicine

Takagi

2014

Bioreactor Engineering Research and Industrial Applications II

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The cell processing engineering including automatic cell processing and noninvasive cell quality estimation of adherent mammalian cells for regenerative medicine was reviewed. Automatic cell processing necessary for the industrialization of regenerative medicine was introduced. The cell quality such as cell heterogeneity should be noninvasively estimated before transplantation to patient, because cultured cells are usually not homogeneous but heterogeneous and most protocols of regenerative medicine are autologous system. The differentiation level could be estimated by two-dimensional cell morphology analysis using a conventional phase-contrast microscope. The phase-shifting laser microscope (PLM) could determine laser phase shift at all pixel in a view, which is caused by the transmitted laser through cell, and might be more noninvasive and more useful than the atomic force microscope and digital holographic microscope. The noninvasive determination of the laser phase shift of a cell using a PLM was carried out to determine the three-dimensional cell morphology and estimate the cell cycle phase of each adhesive cell and the mean proliferation activity of a cell population. The noninvasive discrimination of cancer cells from normal cells by measuring the phase shift was performed based on the difference in cytoskeleton density. Chemical analysis of the culture supernatant was also useful to estimate the differentiation level of a cell population. A probe beam, an infrared beam, and Raman spectroscopy are useful for diagnosing the viability, apoptosis, and differentiation of each adhesive cell.

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Correlation between actin content and laser phase shift of adhesive normal and malignant prostate epithelial cells

Takagi

Tokunaga

2013

Journal of Bioscience and Bioengineering

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Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells

Cited by 3 publications

References 9 publications

Noninvasive discrimination between human normal and cancer cells by analysis of intracellular distribution of phase-shift data

Noninvasive discrimination between human normal and cancer cells by analysis of intracellular distribution of phase-shift data

Cell Processing Engineering for Regenerative Medicine

Correlation between actin content and laser phase shift of adhesive normal and malignant prostate epithelial cells

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