Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila

Araújo, Sofia J.; Llimargas, Marta

doi:10.1007/978-1-0716-2887-4_11

Cited by 3 publications

(3 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the confocal microscope, focus on one of the embryos of interest. For a detailed protocol on how to perform live imaging of Drosophila embryos, refer to Araújo and Llimargas (2023) .…”

Section: Methodsmentioning

confidence: 99%

Recoil Measurements in <em>Drosophila</em> Embryos: from Mounting to Image Analysis

Sánchez-Cisneros,

Bhide,

Ríos-Barrera

2023

BIO-PROTOCOL

View full text Add to dashboard Cite

Tension and force propagation play a central role in tissue morphogenesis, as they enable sub- and supra-cellular shape changes required for the generation of new structures. Force is often generated by the cytoskeleton, which forms complex meshworks that reach cell–cell or cell–extracellular matrix junctions to induce cellular rearrangements. These mechanical properties can be measured through laser microdissection, which concentrates energy in the tissue of interest, disrupting its cytoskeleton. If the tissue is undergoing tension, this cut will induce a recoil in the surrounding regions of the cut. This protocol describes how one can perform laser microdissection experiments and subsequently measure the recoil speed of the sample of interest. While we explain how to carry out these experiments in Drosophila embryos , the recoil calibration and downstream analyses can be applied to other types of preparations. Key features Allows measuring tension in live Drosophila embryos with a relatively simple approach. Describes a quick way to mount a high number of embryos. Includes a segmentation-free recoil quantification that reduces bias and speeds up analysis. Graphical overview

show abstract

“…In the confocal microscope, focus on one of the embryos of interest. For a detailed protocol on how to perform live imaging of Drosophila embryos, refer to Araújo and Llimargas (2023) .…”

Section: Methodsmentioning

confidence: 99%

Recoil Measurements in <em>Drosophila</em> Embryos: from Mounting to Image Analysis

Sánchez-Cisneros,

Bhide,

Ríos-Barrera

2023

BIO-PROTOCOL

View full text Add to dashboard Cite

show abstract

“…Yet, with the exception of a few studies by ourselves and others (Azizoglu et al 2017; Shih et al 2016; Löf-Öhlin et al 2017; Darrigrand et al 2024), the majority of studies on murine pancreatic tubulogenesis have relied on static histochemical analyses. Similarly, most “real time” murine branching studies in other organs have been performed in either low temporal frequency or in 2D (Riccio et al 2016; Mederacke et al 2022; Metzger et al 2008)(Araújo and Llimargas 2023; Mullapudi et al 2019; Scheele et al 2017; Packard et al 2013)(Riccio et al 2016; Mederacke et al 2022; Metzger et al 2008)Meanwhile, high frequency 4D (x, y, z, time) imaging of non-mammalian models of branching organs have also contributed to the advancement of the field (Araújo and Llimargas 2023; Mullapudi et al 2019; Scheele et al 2017; Packard et al 2013). Here we bridge a gap and advance our understanding of mammalian tubulogenesis, by quantifying the dynamics of lumenal network development through analysis of high temporal frequency 4D data.…”

Section: Introductionmentioning

confidence: 99%

“…This can only be achieved through analysis of high frequency 4D (x, y, z, time) data. Most "real time" branching studies have been performed in either low frequency or only in 2D (Riccio et al 2016;Mederacke et al 2022;Metzger et al 2008), but a few high frequency 4D studies in other species and models of branching organs exist and have contributed to the advancement of the field (Araújo and Llimargas 2023;Mullapudi et al 2019;Scheele et al 2017;Packard et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Real time quantification reveals novel dynamic processes in pancreatic lumenal network establishment and remodeling

Jackson,

Heilmann,

Ebeid

et al. 2023

Preprint

View full text Add to dashboard Cite

During embryogenesis dynamic changes in tissue architecture transform primitive anlages to functional organs. Here we document in real time how pancreatic lumens are derived and transformed using a new apical-polarity mouse reporter. Our 4D imaging data reveals dynamic remodeling of apical proteins and lumens to primarily drive each stage of pancreatic duct development. Furthermore, we pinpoint two unique transitions during lumenogenesis. Contrary to current de novo models of polarity acquisition, we show that expansion and rearrangement of the pre-existing central primary lumen drives early network growth. We also document how the endocrine promoting niche - a plexus of interconnected ducts - is resolved. We show that an arborized network forms by gradual closing of ductal loops, rather than via pruning. These highly novel tissue dynamics provide a new framework within which cell and molecular signaling can be investigated in order to better understand the interplay between organ architecture and cell fate.

show abstract

Morphometric Image Analysis and its Applications in Biomedicine Using Different Microscopy Modes

Merhar,

Naicker

2023

Biomedical Visualization

View full text Add to dashboard Cite

Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila

Cited by 3 publications

References 31 publications

Recoil Measurements in <em>Drosophila</em> Embryos: from Mounting to Image Analysis

Recoil Measurements in <em>Drosophila</em> Embryos: from Mounting to Image Analysis

Real time quantification reveals novel dynamic processes in pancreatic lumenal network establishment and remodeling

Morphometric Image Analysis and its Applications in Biomedicine Using Different Microscopy Modes

Contact Info

Product

Resources

About