2020
DOI: 10.3791/60750
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Time-lapse Imaging of Mouse Macrophage Chemotaxis

Abstract: Chemotaxis is receptor-mediated guidance of cells along a chemical gradient, whereas chemokinesis is the stimulation of random cell motility by a chemical. Chemokinesis and chemotaxis are fundamental for the mobilization and deployment of immune cells. For example, chemokines (chemotactic cytokines) can rapidly recruit circulating neutrophils and monocytes to extravascular sites of inflammation. Chemoattractant receptors belong to the large family of G protein-coupled receptors. How chemoattractant (i.e., liga… Show more

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Cited by 10 publications
(2 citation statements)
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References 33 publications
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“…In our collagen hydrogels, macrophages tend to migrate in a range of 0.05-0.2 μm/min, reaching velocities of 0.5 μm/min in the lowest concentration gels. In contrast, in vitro assays where macrophages migrated on 2D surfaces, velocities around 1 μm/min have been observed [36]. In vivo fish models, similar results have been obtained, with velocities in the range of 1-2.5 μm/min [37], which can increase over 10 μm/min in the presence of a wound [38].…”
Section: Macrophage Migration and Velocity Are Inversely Proportional...supporting
confidence: 60%
“…In our collagen hydrogels, macrophages tend to migrate in a range of 0.05-0.2 μm/min, reaching velocities of 0.5 μm/min in the lowest concentration gels. In contrast, in vitro assays where macrophages migrated on 2D surfaces, velocities around 1 μm/min have been observed [36]. In vivo fish models, similar results have been obtained, with velocities in the range of 1-2.5 μm/min [37], which can increase over 10 μm/min in the presence of a wound [38].…”
Section: Macrophage Migration and Velocity Are Inversely Proportional...supporting
confidence: 60%
“…Stable gradients + [38] +/− [7,38] − + [39,40] + [17] Single-cell tracking − + [31,41] + [31] + [39,40] + [28,36] Fugetaxis detection + [42,43] − + [44] + [45] + [17] Distinction between chemotaxis and chemokinesis + [46] + [41,47] +/− [48] + [39,40] + High throughput +/− [49,50] − − + [13,51] + Specialized equipment +/− [6,50] − − + [39,40] +/− Real-time observation +/− [6] + [41,47] + [31,48] + [39,40] + [17] Multiple cell types − − +/− [48] +/− [51] + Patterning cells − − − +/− [39] + Multiple chemoattractants − − + [48] + [39,51] + Cell isolation/circuit reconfiguration − − − − + [37] Targeted live cell recovery − − − − +…”
Section: Fluid-walled Microfluidicsmentioning
confidence: 99%