“…However, the distinctness of A. florea and A. andreniformis as unequivocal biological species has now been well established based on drone morphology (Ruttner, 1975;Kuang and Li, 1985;Kuang, 1986, 1987;Ruttner, 1988;Wongsiri et al, 1990;Chen, 1993), nest structure (Dung et al, 1996;Rinderer et al, 1996), morphometrics (Rinderer et al, 1995), allozyme polymorphism (Nunamaker et al, 1984;Li et al, 1986;Gan et al, 1991), mtDNA sequence divergences (Smith, 1991;Nanork et al, 2001;360 H.R. Hepburn et al Takahashi et al, unpublished data), and differences in the timing of mating flights (Rinderer et al, 1993). Several of these differences contribute to complete reproductive isolation between the two species Dung et al, 1996).…”