Time of ovulation in goats (Capra hircus) induced to superovulate with PMSG

Cameron, A. W. N.; Battye, K. M.; Trounson, Alan

doi:10.1530/jrf.0.0830747

Cited by 35 publications

(13 citation statements)

References 5 publications

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“…Furthermore, unfertilised ova may also be attributed to the abnormal maturation of oocytes, due to premature activation of the initial stages of meiotic oocyte maturation. This abnormality usually occurs as a result of increased blood LH concentrations following eCG superovulation treatment -as this hormone exhibits both LH and FSH bioactivity (Cameron et al, 1988 andKumar et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Lehloenya

2013

Small Ruminant Research

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This study was conducted to evaluate the use of one shot follicle stimulating hormone (FSH) plus equine chorionic gonadotrophin (eCG) (simplified) superovulation protocol in mature Boer goat does. Eighteen does used were allocated into two groups, during the natural breeding season (autumn). The two group were Group 1: the control (n = 9); multiple FSH protocol and Group 2: treatment group (n = 9); one shot FSH plus eCG protocol. All does were synchronised for oestrus, using controlled internal drug release dispensers (CIDR's) for 9 days. Does in the multiple FSH protocol group were superovulated with 200 μg pFSH/doe, administered i.m. in 7 dosages at 12 h intervals, starting 48 h prior to CIDR removal. In the treatment group (one shot FSH plus eCG protocol), the superovulation treatment was administered once as 200 μg FSH plus 350 IU eCG, 48 h prior to CIDR removal. All does were then observed for oestrous behaviour twice daily, at 12 h intervals following CIDR removal for a period of 72 h. Cervical inseminations (with 0.1 ml fresh undiluted Boer goat semen) were performed 36 h and 48 h following CIDR removal. Embryos were then surgically flushed on day 6 following the second artificial insemination. The time interval from CIDR removal to onset of oestrus was not affected by treatment. The duration of the oestrous period however was significantly longer in the one shot group, compared to the multiple FSH protocol. Does from the one shot FSH plus eCG protocol recorded a significantly (p < 0.05) lower mean number of CL's, structures (unfertilised ova plus embryos) and (p < 0.01) embryos recovered. A significantly (p < 0.01) higher number of unfertilised ova were observed from the one shot FSH plus eCG group (4.06 ± 0.9), compared to the multiple FSH group (0.24 ± 0.1). However, the number of degenerated embryos was not affected by treatment. The total number of transferable embryos was significantly lower in the one shot FSH plus eCG protocol (0.22 ± 0.2), compared to the multiple FSH protocol (9.56 ± 1.9). The size of follicles was not affected by treatment, however the total number of follicles at CIDR removal was significantly lower in the one shot FSH plus eCG group (11.67 ± 0.7), compared to the multiple FSH group (14.83 ± 0.7). AI might have been performed at the wrong time, because of the duration of the oestrous period being longer in Group 2. The multiple FSH superovulation protocol overall seemed to be more efficient in the Boer goat. More research is needed on the doses of gonadotrophins administered and the time of AI, in order for the simplified protocol (Group 2) to be effective.

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Section: Discussionmentioning

confidence: 99%

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Lehloenya

2013

Small Ruminant Research

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“…In this regard, mouflons maintained at similar environmental conditions have a higher number of ovarian cycles (mean 10 cycles) of normal duration [14] than that detected in the ibex of the current study. Most investigators who have examined the role of stress in ovulatory activity have considered that stress appears to delay or abolish ovulation in some females [28,29]. Specifically, stress reduces the normal pulsatile patterns of GnRH and, consequently, suppresses or delays the surge of LH [30,31].…”

Section: Discussionmentioning

confidence: 99%

Seasonal ovulatory activity and plasma prolactin concentrations in the Spanish ibex(Capra pyrenaica hispanica) maintained in captivity

Santiago-Moreno¹,

Gómez-Brunet²,

González-Bulnes³

et al. 2003

Reprod. Nutr. Dev.

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-Seasonal changes in ovulatory activity, assessed by the measurement of plasma progesterone and plasma prolactin concentrations were monitored in 10 Spanish ibex females captured in the National Wildlife Reserve of Sierra Nevada (South Spain, 37º N). Five of the 10 female ibex showed ovulatory activity with a mean (± s.e.m.) duration of the oestrous cycle of 19.4 ± 1 days (range: 17-23 days). On average, the five cyclic females weighed 28 ± 0.9 kg. Progesterone cycles occurred only in animals older than 4 years of age. Ovulatory activity extended from December to January. The duration of the breeding season was 43.2 ± 7.7 days. Ibex females younger than 4 years of age had a body weight lower (P < 0.01) than that of adults and none of them displayed ovulations. Plasma concentrations of prolactin levels were significantly affected by season (P < 0.05), following a trend that was roughly parallel to daylength. The highest values occurred in the spring (119.7 ± 21.4 ng·mL -1 ) and the summer (139.3 ± 19.8 ng·mL -1 ), the lowest values in the autumn (26 ± 7.4 ng·mL -1 ) and in the winter (19.7 ± 3.2 ng·mL -1 ). Our results showed a very restricted breeding season, despite the fact that the Spanish ibex originates from and lives in a temperate latitude, revealing a remarkably good adaptation to the harsh climatic and nutritional conditions of their mountainous habitat.breeding season / ovulation / progesterone

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“…The occurrence of the low pregnancy rates have been attributed to vaginal inflammation (e.g. vaginitis) by intravaginal progestagens and stress induced fertilization failure (Cameron et al, 1988;Macfarlane et al, 2000). Likewise, the variability in response to hormonal treatments used in synchronization is known to limit fertility (Maurel et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the advantages of this «simplified» method were that vaginal manipulation (and potentially vaginitis) was avoided and the deleterious effects derived from handling stress were minimized. In this sense, it is well known that stress induced by handling during synchronization treatments can lead to delayed or abolished ovulation in some females (Cameron et al, 1988;Macfarlane et al, 2000), or to an early luteal regression (Loskutoff et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Short communication. Synchronization of ovulation and artificial insemination protocol for Spanish ibex (Capra pyrenaica) based in progesterone and cloprostenol

Santiago-Moreno

Toledano-Dı́az

Gómez-Brunet

et al. 2008

Span. j. agric. res.

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The Spanish ibex (Capra pyrenaica) is an Iberian mountain ungulate that is listed as a threatened species, and thus is an ideal candidate for reproductive technologies that might enhance breeding programmes in this species. Previous studies have reported success in collecting viable epididymal spermatozoa from dead Spanish ibex males and subsequently artificially inseminating ibex females synchronized by inserting intravaginal progestagens for 11 days in conjunction with injections of equine chorionic gonadotropin (eCG) and cloprostenol two days before progestagen withdrawal (Santiago-Moreno et al., 2006a AbstractThis study describes the effectiveness of a method to synchronize ovulation in Spanish ibex for artificial insemination, based on one previously described for use in domestic goats. Estrus and ovulation were synchronised in eight adult ibex females (aged 3-5 yr old) during the breeding season (January) by i.m. injection of 25 mg of progesterone in olive oil plus an 100 µg i.m. of cloprostenol on day 0 followed by a single dose of 100 µg cloprostenol i.m. 9 days later (day 10). Ibexes were inseminated by laparoscopy with 200 × 10 6 spermatozoa at 52 h after the second cloprostenol injection. Two of the eight treated ibex (25%) conceived and carried their pregnancies to term. The protocol described here minimizes animal handling (in contrast to synchronization methods utilizing intravaginal progestins and equine chorionic gonadotropin injections), which greatly facilitates the application of reproductive technologies in Spanish ibex captive breeding programs.

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Time of ovulation in goats (Capra hircus) induced to superovulate with PMSG

Cited by 35 publications

References 5 publications

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Seasonal ovulatory activity and plasma prolactin concentrations in the Spanish ibex(Capra pyrenaica hispanica) maintained in captivity

Short communication. Synchronization of ovulation and artificial insemination protocol for Spanish ibex (Capra pyrenaica) based in progesterone and cloprostenol

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