Time of sampling is crucial for measurement of cell-free plasma DNA following acute aseptic inflammation induced by exercise

Fatouros, Ioannis G.; Jamurtas, Athanasios Z.; Nikolaidis, Michalis G.; Destouni, Aspasia; Michailidis, Yiannis; Vrettou, Christina; Douroudos, Ioannis; Avloniti, Alexandra; Chatzinikolaou, Athanasios; Taxildaris, Kyriakos; Kanavakis, Emmanouel; Papassotiriou, Ioannis; Kouretas, Demetrios

doi:10.1016/j.clinbiochem.2010.08.020

Cited by 65 publications

(60 citation statements)

References 10 publications

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“…Other studies confirmed the very short half-life of cfDNA in blood stream. For example, Fatouros and colleagues have measured the kinetics of cfDNA concentrations in athletes following vigorous exercise and reported that cfDNA increased to 15-fold postexercise, stabilized at 13-fold for 30 minutes after exercise and normalized 30 minutes later (37). In short, there is no controversy on the kinetics of cfDNA clearance from blood stream; however, the mechanisms of its clearance have not been studied in detail.…”

Section: Properties Of Cfdnamentioning

confidence: 99%

Cell-free DNA (cfDNA): Clinical Significance and Utility in Cancer Shaped By Emerging Technologies

Volik¹,

Alcaide

Morin

et al. 2016

Molecular Cancer Research

300

235

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Precision oncology is predicated upon the ability to detect specific actionable genomic alterations and to monitor their adaptive evolution during treatment to counter resistance. Because of spatial and temporal heterogeneity and comorbidities associated with obtaining tumor tissues, especially in the case of metastatic disease, traditional methods for tumor sampling are impractical for this application. Known to be present in the blood of cancer patients for decades, cell-free DNA (cfDNA) is beginning to inform on tumor genetics, tumor burden, and mechanisms of progression and drug resistance. This substrate is amenable for inexpensive noninvasive testing and thus presents a viable approach to serial sampling for screening and monitoring tumor progression. The fragmentation, low yield, and variable admixture of normal DNA present formidable technical challenges for realization of this potential. This review summarizes the history of cfDNA discovery, its biological properties, and explores emerging technologies for clinically relevant sequence-based analysis of cfDNA in cancer patients. Molecular barcoding (or Unique Molecular Identifier, UMI)-based methods currently appear to offer an optimal balance between sensitivity, flexibility, and cost and constitute a promising approach for clinically relevant assays for near real-time monitoring of treatment-induced mutational adaptations to guide evidence-based precision oncology.

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Section: Properties Of Cfdnamentioning

confidence: 99%

Cell-free DNA (cfDNA): Clinical Significance and Utility in Cancer Shaped By Emerging Technologies

Volik¹,

Alcaide

Morin

et al. 2016

Molecular Cancer Research

300

235

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“…In the same study, cf-DNA concentrations peaked immediately after exercise and returned to the pre-exercise baseline within two hours after the treadmill protocol. 19 Akin to the postendurance exercise response, the concentration of cf-DNA follows a similar time course after a single bout of resistance training. 13 Prior to this study, the authors are aware of only a single investigation addressing cf-DNA concentrations in response to chronic changes in exercise workload.…”

Section: Introductionmentioning

confidence: 99%

“…13,14,16,19,20 The characteristic timecourse of cf-DNA after a single exercise session may offer benefits over common markers of tissue damage and inflammation. For example, during an investigation employing a protocol of exhaustive treadmill running, C-reactive protein (CRP), uric acid (UA) and creatine kinase (CK) concentrations increased until the final blood draw 24 hours post-exercise.…”

Section: Introductionmentioning

confidence: 99%

Changes in cell free DNA during a college soccer season

Gentles¹,

Hornsby²,

Gray³

et al. 2015

Journal of Trainology

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Objectives: This study investigated chronic changes in cell free DNA (cf-DNA) throughout a collegiate soccer season. The relationship between cf-DNA, C-reactive protein (CRP), creatine kinase (CK), testosterone (T), cortisol (C), testosterone-cortisol ratio (T:C), body mass and body composition were also examined. Design: Longitudinal study design with repeated measures and group comparisons. Methods: Twenty three NCAA Division I male soccer players were divided into two groups. Starters were placed in Group 1 (G1) and non-starters were placed in Group 2 (G2). cf-DNA, CRP, CK, T, C, T:C, body mass and body composition were taken three times, corresponding to pre-season, approximately mid-season and immediately after the concluding the season. Results: In G1, cf-DNA, CRP, CK, cf-DNA %∆, CRP %∆ and, CK %∆ were all statistically higher at T2 and T3 than T1. In G2, CRP %∆ was statistically higher at T2 than T1. In G2, cf-DNA %∆, CRP %∆ and CK %∆ were higher at T2 and T3 than T1. Conclusions: This suggests that cf-DNA may be a useful marker that can reflect accumulated soccer training and competitive stressors.(Journal of Trainology 2015;4:25-31)

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“…Intense exercise has recently been reported to provoke increases in the plasma and serum concentrations of circulating cellfree DNA (cf-DNA) 3 (2,3 ), a phenomenon considered a hallmark of various pathologic conditions, including cancer, autoimmune diseases, trauma, sepsis, stroke, and myocardial infarction (4 ). Despite intensive research, the sources, triggers, and mechanisms that cause increases in cf-DNA remain enigmatic.…”

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confidence: 99%

Short-Term Treadmill Running as a Model for Studying Cell-Free DNA Kinetics In Vivo

et al. 2011

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BACKGROUND Increased plasma concentrations of cell-free DNA (cf-DNA) are considered a hallmark of various clinical conditions. Despite intensive research in this field, limited data are available concerning the time course of release and clearance of cf-DNA in vivo. METHODS We extracted cf-DNA from plasma samples taken before and immediately after a 10-km cross-country run, and from samples taken before, immediately after, and 30 min after exhaustive short-term treadmill exercise. The contribution of nuclear (nDNA) and mitochondrial DNA (mtDNA) was measured by quantitative real-time PCR. The incremental treadmill exercise setup was exploited to delineate the precise sequencing and timing of cf-nDNA, lactate, and high-mobility group box 1 protein (HMGB1) release during the exercise and recovery phases. RESULTS Postexercise plasma cf-nDNA concentrations in cross-country and treadmill runners were significantly increased, by 7.6-fold and 9.9-fold, respectively (P < 0.001). cf-nDNA concentrations were not correlated with age, sex, or body mass index. Plasma concentrations of cf-nDNA and HMGB1 in postexercise samples of treadmill runners were significantly correlated (r = 0.84; P = 0.004). cf-mtDNA concentrations were not affected by treadmill exercise. Time-course analyses demonstrated that cf-nDNA is released within minutes after the onset of exercise and is rapidly cleared from the circulation after the cessation of exercise. Nearly congruent kinetics for cf-nDNA, lactate, and HMGB1 were observed during the exercise phase. CONCLUSIONS A single bout of exhaustive short-term treadmill exercise constitutes a versatile model system suitable for addressing basic questions about cf-DNA biology.

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Time of sampling is crucial for measurement of cell-free plasma DNA following acute aseptic inflammation induced by exercise

Cited by 65 publications

References 10 publications

Cell-free DNA (cfDNA): Clinical Significance and Utility in Cancer Shaped By Emerging Technologies

Cell-free DNA (cfDNA): Clinical Significance and Utility in Cancer Shaped By Emerging Technologies

Changes in cell free DNA during a college soccer season

Short-Term Treadmill Running as a Model for Studying Cell-Free DNA Kinetics In Vivo

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