Time-resolved circular dichroism: What can we learn on conformational changes?

Abstract

Time-resolved circular dichroism (CD) measurements can yield relevant information on the dynamics of conformational changes in molecules on the condition that some a priori knowledge is obtained. This can be the use of simple models such as the excitonic coupling or the octant rule or the phenomenological relationship between far-UV CD and secondary structures in proteins for example. This article describes such experiments where CD has brought relevant conformational information.

Multiscale conformational dynamics probed by time‐resolved circular dichroism from seconds to picoseconds

“…For further discussion, see Hache. 26 In other cases, quantum chemical calculations can help interpreting the TRCD measurements. 27,28 This issue is of primary importance for single-wavelength experiments but will be less so when broadband measurements can be performed routinely.…”

“…But it is often important to rely on other piece of information that can be general rules (excitonic coupling, octant rule) or empirical knowledge about the CD spectra expected after irradiation. For further discussion, see [26]. In other cases, quantum chemical calculations can help interpreting the TRCD measurements [27,28].…”