Time-Resolved Fluorescence Immunochromatography Assay (TRFICA) for Aflatoxin: Aiming at Increasing Strip Method Sensitivity

Li, Hui; Wang, Du; Tang, Xiaoqian; Zhang, Wen; Zhang, Qi; Li, Peiwu

doi:10.3389/fmicb.2020.00676

Cited by 24 publications

(13 citation statements)

References 61 publications

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“…On the other hand, the lateral flow immunochromatographic assay provides a semiquantitative signal only and it is not fully applicable for the quantification of a marker, though there have been promising experiments aiming to make the assay suitable for the determination of exact concentrations [ 70 ]. The improved versions of lateral flow immunochromatographic assays can provide fully or partially quantified signals; on the other hand, instrumentation for color density, fluorescence intensity, Raman spectroscopy or other instrumentation is necessary in this case [ 71 , 72 , 73 , 74 , 75 ]. The use of instrumentation would make the lateral flow immunochromatographic assay more applicable for diabetes mellitus, but this also creates material demands on equipment and limits the ability to perform the lateral flow immunochromatographic assay in point-of-care conditions.…”

Section: Biosensors and Bioassays Measuring Hba 1cmentioning

confidence: 99%

Glycated Hemoglobin and Methods for Its Point of Care Testing

Pohanka

2021

Biosensors

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Glycated hemoglobin (HbA1c) is a product of the spontaneous reaction between hemoglobin and elevated glucose levels in the blood. It is included among the so-called advanced glycation end products, of which is the most important for the clinical diagnosis of diabetes mellitus, and it can serve as an alternative to glycemia measurement. Compared to the diagnosis of diabetes mellitus by glycemia, the HbA1c level is less influenced by a short-term problem with diabetes compensation. Mass spectroscopy and chromatographic techniques are among the standard methods of HbA1c level measurement. Compared to glycemia measurement, there is lack of simple methods for diabetes mellitus diagnosis by means of the HbA1c assay using a point-of-care test. This review article is focused on the surveying of facts about HbA1c and its importance in diabetes mellitus diagnosis, and surveying standard methods and new methods suitable for the HbA1c assay under point-of-care conditions. Various bioassays and biosensors are mentioned and their specifications are discussed.

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Section: Biosensors and Bioassays Measuring Hba 1cmentioning

confidence: 99%

Glycated Hemoglobin and Methods for Its Point of Care Testing

Pohanka

2021

Biosensors

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“…Varied novel nanoparticles, such as quantum dots [ 22 ], fluorescent microspheres [ 23 ], magnetic nanoparticles [ 24 ], and up-conversion phosphorescent nanoparticles [ 25 ], have been used as reporters in LFIA to break through the limitation. Among them, fluorescence microspheres suffer from narrow Stokes shift, strong background signals, and photobleaching, influencing the accuracy and sensitivity of detection results [ 26 ]. After the first introduction of Eu (III) complexes by Weissman [ 26 ], the LFIAs with time-resolved fluorescence microspheres (TRFM-based LFIAs) as labels were found to be extremely suitable for rapid on-site detection with high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…Among them, fluorescence microspheres suffer from narrow Stokes shift, strong background signals, and photobleaching, influencing the accuracy and sensitivity of detection results [ 26 ]. After the first introduction of Eu (III) complexes by Weissman [ 26 ], the LFIAs with time-resolved fluorescence microspheres (TRFM-based LFIAs) as labels were found to be extremely suitable for rapid on-site detection with high sensitivity. Thus, time-resolved fluorescence microspheres (TRFM), assembled by encapsulating thousands of the lanthanides [ 27 ] chelated ions into nano-polystyrene microspheres or silica nanoparticles, have gained popularity among the labels in LFIAs [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Immunoassay Based on Time-Resolved Fluorescence Microspheres for Rapid and Quantitative Screening CA199 in Human Serum

Jiao

Peng

Liang

et al. 2022

IJMS

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Carbohydrate antigen 199 (CA199) is a serum biomarker which has certain value and significance in the diagnosis, prognosis, treatment, and postoperative monitoring of cancer. In this study, a lateral flow immunoassay based on europium (III) polystyrene time-resolved fluorescence microspheres (TRFM-based LFIA), integrated with a portable fluorescence reader, has been successfully establish for rapid and quantitative analysis of CA199 in human serum. Briefly, time-resolved fluorescence microspheres (TRFMs) were conjugated with antibody I (Ab1) against CA199 as detection probes, and antibody II (Ab2) was coated as capture element, and a “TRFMs-Ab1-CA199-Ab2” sandwich format would form when CA199 was detected by the TRFM-based LFIA. Under the optimal parameters, the detection limit of the TRFM-based LFIA for visible quantitation with the help of an ultraviolet light was 4.125 U/mL, which was four times lower than that of LFIA based on gold nanoparticles. Additionally, the fluorescence ratio is well linearly correlated with the CA199 concentration (0.00–66.0 U/mL) and logarithmic concentration (66.0–264.0 U/mL) for quantitative detection. Serum samples from 10 healthy people and 10 liver cancer patients were tested to confirm the performances of the point-of-care application of the TRFM-based LFIA, 20.0 U/mL of CA199 in human serum was defined as the threshold for distinguishing healthy people from liver cancer patients with an accuracy of about 60%. The establishment of TRFM-based LFIA will provide a sensitive, convenient, and efficient technical support for rapid screening of CA199 in cancer diagnosis and prognosis.

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“…Labelled with lanthanide chelates, time-resolved fluoroimmunoassay (TRFIA) have attracted attention due to several advantages over visual labels, such as increased sensitivity and reduced background noise due to a large Stokes shift and long fluorescence lifetime (Dong et al, 2020). This technique has been successfully applied for screening microbial antigens, mycotoxins, and antibiotics in agricultural products and clinical samples (Li et al, 2020;Salminen et al, 2019;Xia et al, 2009;Wang, Sun, et al, 2020). Nevertheless, there is currently no lateral flow immunoassay for detecting A. longipes.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive time‐resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco

Xiong

et al. 2021

J of Applied Microbiology

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Aims: Alternaria longipes is a causal agent of brown spot of tobacco, which remains a serious threat to tobacco production. Herein, we established a detection method for A. longipes in tobacco samples based on the principle of time-resolved fluoroimmunoassay, in order to fulfil the requirement of rapid, sensitive and accurate detection in situ. Methods and Results:A monoclonal antibody against A. longipes was generated, and its purity and titration were assessed using western blot and ELISA. The size of europium (III) nanospheres was measured to confirm successful antibody conjugation. The method described here can detect A. longipes protein lysates as low as 0.78 ng ml −1 , with recovery rates ranging from 85.96% to 99.67% in spiked tobacco.The specificity was also confirmed using a panel of microorganisms. Conclusions:The fluorescent strips allow rapid and sensitive onsite detection of A.longipes in tobacco samples, with high accuracy, specificity, and repeatability.Significance and Impact of the Study: This novel detection method provides convenience of using crude samples without complex procedures, and therefore allows rapid onsite detection by end users and quick responses towards A. longipes, which is critical for disease control and elimination of phytopathogens.

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Time-Resolved Fluorescence Immunochromatography Assay (TRFICA) for Aflatoxin: Aiming at Increasing Strip Method Sensitivity

Cited by 24 publications

References 61 publications

Glycated Hemoglobin and Methods for Its Point of Care Testing

Glycated Hemoglobin and Methods for Its Point of Care Testing

Lateral Flow Immunoassay Based on Time-Resolved Fluorescence Microspheres for Rapid and Quantitative Screening CA199 in Human Serum

Highly sensitive time‐resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco

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