Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts

Fatakdawala, Hussain; Griffiths, Leigh G.; Humphrey, Sterling; Marcu, Laura

doi:10.1117/1.jbo.19.8.080503

Cited by 8 publications

(9 citation statements)

References 9 publications

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“…This makes lifetime measurements robust and reproducible from multiple measurements (with varying experimental setups), making it a promising tool for clinical translation. Our previous work has explored the application of fluorescence lifetime imaging (FLIm) for oral carcinoma detection [9], atherosclerotic plaque assessment [10] and characterization of engineered tissues [11]. More recently we have also reported the ability to measure lifetime from tissue and to augment white-light video frames in real-time [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study

Fereidouni¹,

Gorpas

et al. 2017

Methods Appl. Fluoresc.

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Fluorescence lifetime imaging has been shown to serve as a valuable tool for interrogating and diagnosis of biological tissue at a mesoscopic level. The ability to analyze fluorescence decay curves to extract lifetime values in real-time is crucial for clinical translation and applications such as tumor margin delineation or intracoronary imaging of atherosclerotic plaques. In this work, we compare the performance of two popular non-parametric (fit-free) methods for determining lifetime values from fluorescence decays in real-time –the Phasor approach and Laguerre deconvolution. We demonstrate results from simulated and experimental data to compare the accuracy and speed of both methods and their dependence on noise and model parameters.

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Section: Introductionmentioning

confidence: 99%

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study

Fereidouni¹,

Gorpas

et al. 2017

Methods Appl. Fluoresc.

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“…[151,152] Morphological analysis of bioprinted and matured tissue constructs are generally carried out through histology and immunohistochemistry. [153] Both have been used for end-point analysis following standard steps such as tissue fixation, drying, slicing,and staining. Va rious staining methods,such as hematoxylin and eosin (H&E) or Massons trichrome,a re regularly used for histological analysis.I mmunohistochemistry (IHC) is more specific and uses antibodies against certain epitopes for the identification and assessment of the distribution of target features within at issue section.…”

Section: Viability and Morphologymentioning

confidence: 99%

3D Bioprinting of Tissue/Organ Models

2016

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In vitro tissue/organ models are useful platforms that can facilitate systematic, repetitive, and quantitative investigations of drugs/chemicals. The primary objective when developing tissue/organ models is to reproduce physiologically relevant functions that typically require complex culture systems. Bioprinting offers exciting prospects for constructing 3D tissue/organ models, as it enables the reproducible, automated production of complex living tissues. Bioprinted tissues/organs may prove useful for screening novel compounds or predicting toxicity, as the spatial and chemical complexity inherent to native tissues/organs can be recreated. In this Review, we highlight the importance of developing 3D in vitro tissue/organ models by 3D bioprinting techniques, characterization of these models for evaluating their resemblance to native tissue, and their application in the prioritization of lead candidates, toxicity testing, and as disease/tumor models.

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“…Morphologische Analysen durch Biodruck erzeugter und gereifter Gewebekonstrukte beruhen im Allgemeinen auf Histologie und Immunhistochemie . Beide Ansätze bedürfen einer Probenbehandlung durch Schritte wie Gewebefixierung, Trocknung, Dünnschnitt und Anfärben.…”

Section: Charakterisierung Von In‐vitro‐gewebemodellen Und ‐Organmodunclassified

3D‐Biodruck von Gewebe‐ und Organmodellen

2016

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Gewebe‐ oder Organmodelle können als nützliche In‐vitro‐Plattformen systematische und quantitative Untersuchungen von Wirkstoffen und Chemikalien erleichtern. Oberstes Ziel bei ihrer Entwicklung ist die Nachbildung der wichtigen physiologischen Funktionen, was üblicherweise komplexe Kultursysteme erforderlich macht. Biodrucktechniken bieten interessante Perspektiven für den Aufbau von Gewebe‐ und Organmodellen, da sie eine reproduzierbare, automatisierte Produktion komplexer lebender Gewebe ermöglichen. Auf diese Weise erhaltene Modelle haben Potenzial für das Screening neuer Verbindungen oder für die Vorhersage von Toxizitäten, da in ihnen die räumliche und chemische Komplexität von nativen Geweben oder Organen nachgebildet werden kann. Hier zeigen wir, wie Gewebe‐ und Organmodelle durch 3D‐Biodruck in vitro hergestellt werden können, wie sich ihre Vergleichbarkeit mit dem nativen Gewebe charakterisieren lässt, und wie sie bei der Auswahl von Leitstrukturen, bei Toxizitätstests und als Krankheits‐ oder Tumormodelle von Nutzen sein können.

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Time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy for nondestructive evaluation of vascular grafts

Cited by 8 publications

References 9 publications

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study

Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study

3D Bioprinting of Tissue/Organ Models

3D‐Biodruck von Gewebe‐ und Organmodellen

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