Time-Resolved Fluorescence Spectroscopy Using Synchrotron Radiation

Munro, Ian; Martin, Margaret M.

doi:10.1007/0-306-47057-8_4

Cited by 1 publication

(1 citation statement)

References 59 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, the lifetime of an excited state is supposed to be only slightly temperature/viscosity dependent (35) in the absence of quenching molecules, as it is for diphenylhexatriene in liposome membranes (36). However, it has been found that for triazine dyes, the fluorescence lifetime increases with an increase in solvent viscosity in a series of n ‐alcanols (37), similarly to our experiments.…”

Section: Discussionsupporting

confidence: 90%

Fluorescence Lifetimes Study of α‐Tocopherol and Biological Prenylquinols in Organic Solvents and Model Membranes

Kruk

Myśliwa-Kurdziel

Jemioła-Rzemińska

et al. 2006

Photochem & Photobiology

View full text Add to dashboard Cite

We have found that for biological prenyllipids, such as plastoquinol-9, alpha-tocopherol quinol, and alpha-tocopherol, the shortest fluorescence lifetimes were found in aprotic solvents (hexane, ethyl acetate) whereas the longest lifetimes were those of ubiquinonol-10 in these solvents. For all the investigated prenyllipids, fluorescence lifetime in alcohols increased along with an increase in solvent viscosity. In a concentrated hexane solution, the lifetimes of prenylquinols considerably decreased. This contrasts with methanol solutions, which is probably due to the self-association of these compounds in aprotic solvents. We have also found a correlation of the Stokes shift of prenyllipids fluorescence with the orientation polarizability of the solvents. Based on data obtained in organic solvents, measurements of the fluorescence lifetimes of prenyllipids in liposomes allowed an estimation of the relative distance of their fluorescent rings from the liposome membrane surface, and was found to be the shortest for alpha-tocopherol quinol in egg yolk phosphatidylcholine liposomes, and increased in the following order: alpha-tocopherol in dipalmitoyl phosphatidylcholine liposomes < alpha-tocopherol < plastoquinol-9 < ubiquinol-10 in egg-yolk phosphatidylcholine liposomes.

show abstract

Section: Discussionsupporting

confidence: 90%