Time-resolved in situ synchrotron-microCT: 4D deformation of bone and bone analogues using digital volume correlation

Fernández, Marta Peña; Kao, Alexander P.; Bonithon, Roxane; Howells, David; Bodey, Andrew J.; Wanelik, Kaz; Witte, Frank; Johnston, Richard; Arora, Hari; Tozzi, Gianluca

doi:10.1016/j.actbio.2021.06.014

Cited by 32 publications

(18 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This applied compression results in the deformation not only of the bone specimens, but also the endcaps and the loading system. As such, apparent yielding and/or failure of the bone specimens was not reached at 3% applied compression, similarly to previous in situ XCT studies (Nazarian and Müller, 2004; Peña Fernández et al, 2021; Turunen et al, 2020). During the acquisition of the XCT images small changes in the displacement fields are expected (Peña Fernández et al, 2021), while the inherent time-dependent behaviour of bone tissue leads to a redistribution of stresses in the specimen.…”

Section: Discussionsupporting

confidence: 89%

“…was not reached at 3% applied compression, similarly to previous in situ XCT studies (Nazarian and Müller, 2004;Peña Fernández et al, 2021;Turunen et al, 2020). During the acquisition of the XCT images small changes in the displacement fields are expected (Peña Fernández et al, 2021) , while the inherent time-dependent behaviour of bone tissue leads to a redistribution of stresses in the specimen.…”

Section: Limitationssupporting

confidence: 83%

“…However, those approaches would not allow tracking of the microdamage mechanism in one specimen. In contrast, in situ XCT experiments have shown great potential to evaluate crack patterns in bone tissue under step-wise or continuous mechanical loading (Lu et al, 2019; Peña Fernández et al, 2021, 2020b, 2019). In this study, even if the specimens did not reach the apparent yield point, microcracks were detected in regions were microdamage was predicted by the nonlinear µFE models (Figure 6).…”

Section: Discussionmentioning

confidence: 99%

“…To date, DVC remains the only available experimental technique providing 3D measurements of local displacements and derived strains within bone structures (Peña Fernández et al, 2021), and it has been previously used to validate local displacements predicted by linear elastic μFE models of bone structures (Chen et al, 2017; Fu et al, 2021; Hussein et al, 2018; Kusins et al, 2019; Palanca et al, 2022; Zauel et al, 2006), showing excellent correlation between experimental and predicted values when DVC displacements are used as boundary conditions for μFE models. However, bone displays a linear elastic-viscoplastic behaviour, where localised microdamage may occur even within the apparent elastic regime (Morgan et al, 2003; Schwiedrzik and Zysset, 2013; Stipsitz et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nonlinear micro finite element models based on digital volume correlation measurements predict early microdamage in newly formed bone

Fernández

Sasso

McPhee

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Bone regeneration in critical-sized defects is a clinical challenge, with biomaterials under constant development aiming at enhancing the natural bone healing process. The delivery of bone morphogenetic proteins (BMPs) in appropriate carriers represents a promising strategy for bone defect treatment but optimisation of the spatial-temporal release is still needed for the regeneration of bone with biological, structural, and mechanical properties comparable to the native tissue. Nonlinear micro finite element (μFE) models can address some of these challenges by providing a tool able to predict the biomechanical strength and microdamage onset in newly formed bone when subjected to physiological or supraphysiological loads. Yet, these models need to be validated against experimental data. In this study, experimental local displacements in newly formed bone induced by osteoinductive biomaterials subjected to in situ X-ray computed tomography compression in the apparent elastic regime and measured using digital volume correlation (DVC) were used to validate μFE models. Displacement predictions from homogeneous linear μFE models were highly correlated to DVC-measured local displacements, while tissue heterogeneity capturing mineralisation differences showed negligible effects. Nonlinear μFE models improved the correlation and showed that tissue microdamage occurs at low apparent strains. Microdamage seemed to occur next to large cavities or in biomaterial-induced thin trabeculae, independent of the mineralisation. While localisation of plastic strain accumulation was similar, the amount of damage accumulated in these locations was slightly higher when including material heterogeneity. These results demonstrate the ability of the nonlinear μFE model to capture local microdamage in newly formed bone tissue and can be exploited to improve the current understanding of healing bone and mechanical competence. This will ultimately aid the development of BMPs delivery systems for bone defect treatment able to regenerate bone with optimal biological, mechanical, and structural properties.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Limitationssupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nonlinear micro finite element models based on digital volume correlation measurements predict early microdamage in newly formed bone

Fernández

Sasso

McPhee

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, such an approach does not provide information on how the deformation of internal structure influences the overall mechanical response of an APM foam element. A possible solution is the use of advanced radiographical imaging methods to reveal the deformation processes within the microstructure of the specimen during a loading procedure [ 3 , 20 , 21 , 22 ]. Herein, the time-resolved 4D µCT experiments based on in-situ loading of a specimen in the X-ray scanner that is used for its simultaneous imaging enables capturing the deforming microstructure of the observed APM sample.…”

Section: Introductionmentioning

confidence: 99%

Fast 4D On-the-Fly Tomography for Observation of Advanced Pore Morphology (APM) Foam Elements Subjected to Compressive Loading

et al. 2021

View full text Add to dashboard Cite

Observation of dynamic testing by means of X-ray computed tomography (CT) and in-situ loading devices has proven its importance in material analysis already, yielding detailed 3D information on the internal structure of the object of interest and its changes during the experiment. However, the acquisition of the tomographic projections is, in general, a time-consuming task. The standard method for such experiments is the time-lapse CT, where the loading is suspended for the CT scan. On the other hand, modern X-ray tubes and detectors allow for shorter exposure times with an acceptable image quality. Consequently, the experiment can be designed in a way so that the mechanical test is running continuously, as well as the rotational platform, and the radiographic projections are taken one after another in a fast, free-running mode. Performing this so-called on-the-fly CT, the time for the experiment can be reduced substantially, compared to the time-lapse CT. In this paper, the advanced pore morphology (APM) foam elements were used as the test objects for in-situ X-ray microtomography experiments, during which series of CT scans were acquired, each with the duration of 12 s. The contrast-to-noise ratio and the full-width-half-maximum parameters are used for the quality assessment of the resultant 3D models. A comparison to the 3D models obtained by time-lapse CT is provided.

show abstract

In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

Dejea,

Pierantoni,

Orozco

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Articular cartilage and meniscus transfer and distribute mechanical loads in the knee joint. Degeneration of these connective tissues occurs during the progression of knee osteoarthritis, which affects their composition, microstructure, and mechanical properties. A deeper understanding of disease progression can be obtained by studying them simultaneously. Time‐resolved synchrotron‐based X‐ray phase‐contrast tomography (SR‐PhC‐µCT) allows to capture the tissue dynamics. This proof‐of‐concept study presents a rheometer setup for simultaneous in situ unconfined compression and SR‐PhC‐µCT of connective knee tissues. The microstructural response of bovine cartilage (n = 16) and meniscus (n = 4) samples under axial continuously increased strain, or two steps of 15% strain (stress–relaxation) is studied. The chondrocyte distribution in cartilage and the collagen fiber orientation in the meniscus are assessed. Variations in chondrocyte density reveal an increase in the top 40% of the sample during loading, compared to the lower half. Meniscus collagen fibers reorient perpendicular to the loading direction during compression and partially redisperse during relaxation. Radiation damage, image repeatability, and image quality assessments show little to no effects on the results. In conclusion, this approach is highly promising for future studies of human knee tissues to understand their microstructure, mechanical response, and progression in degenerative diseases.

show abstract

Time-resolved in situ synchrotron-microCT: 4D deformation of bone and bone analogues using digital volume correlation

Cited by 32 publications

References 94 publications

Nonlinear micro finite element models based on digital volume correlation measurements predict early microdamage in newly formed bone

Nonlinear micro finite element models based on digital volume correlation measurements predict early microdamage in newly formed bone

Fast 4D On-the-Fly Tomography for Observation of Advanced Pore Morphology (APM) Foam Elements Subjected to Compressive Loading

In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

Contact Info

Product

Resources

About