Time-resolved microfluorometric study of the binding sites of lipophilic cationic pyrene probes in mitochondria of living HeLa cells

Hüglin, D.; Seiffert, W.; Zimmermann, Herbert

doi:10.1016/1011-1344(95)07191-1

Cited by 25 publications

(9 citation statements)

References 34 publications

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“…It is nowadays used for membrane fluidity detection [3] and oxygen sensing film [4]. Other pyrene derivatives allow measurement of oxygen concentrations in living cells by the detection of fluorescence intensity variations [5,6] or fluorescence lifetime changes [7]. Lifetime measurements are not dependent on the absolute intensity of the emitted light and, therefore, independent of the probe concentration.…”

Section: Introductionmentioning

confidence: 99%

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Rharass

Ribou²,

Vigo³

et al. 2005

Free Radical Research

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We investigated the fluorescence lifetime of pyrene butyric acid (PBA) using various O2 concentrations in cells. Both in living and freshly fixed cells, PBA lifetime decreased with oxygen concentration. We recorded decay curves in single cells and measured PBA lifetime and NAD(P)H intensity values. Under nitrogen atmosphere, the probe lifetime differences (199 and 209 ns in living and freshly fixed cells, respectively) suggest a supplemental pathway for the deactivation of the probe when the cell functions are not stopped. We propose reactive oxygen species (ROS) to be the additional quenchers that cause this decrease. We further studied the effect of drugs generating ROS the anthracycline doxorubicin (adriamycin). For living cells, PBA lifetime decreased after adriamycin (ADR) treatment (200 and 1000 ng/ml). This supports our hypothesis that under nitrogen atmosphere and for freshly fixed cells, PBA lifetimes increase to an unchanging value due to absence of quenchers.

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Section: Introductionmentioning

confidence: 99%

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Rharass

Ribou²,

Vigo³

et al. 2005

Free Radical Research

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“…Lipophilic cationic photosensitizers are known to localize in mitochondria [57] while less lipophilic cationic photosensitizers localize in lysosomes [58]. The second positive charge present on BC-3′ increases the cationic character and thus apparently reduces the lipophilicity sufficiently to alter the localization from mitochondria to lysosomes and thus decreases the PDT efficacy to some extent; however, even BC-3′ still has very high activity versus comparable compounds.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and evaluation of cationic bacteriochlorin amphiphiles with effective in vitro photodynamic activity against cancer cells at low nanomolar concentration

Sharma

Krayer

Sperandio

et al. 2013

J. Porphyrins Phthalocyanines

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Bacteriochlorins are attractive candidates as photosensitizers for photodynamic therapy (PDT) due to their intense absorption in the near-infrared (NIR) region of the spectrum where light transmission through tissue is maximal. Many naturally occurring bacteriochlorins are inherently unstable due to adventitious atmospheric oxidation. A de novo synthesis affords bacteriochlorins that contain a geminal dimethyl group in each reduced pyrrole ring to increase stability against oxidation. Here, three new synthetic bacteriochlorins, each bearing a single side-chain containing one or two positive charges, were investigated for their in vitro PDT activity against HeLa human cancer cells. All bacteriochlorins were active at low nanomolar concentration when activated with NIR light; those bearing a single positive charge exhibited faster uptake and higher activity. The bacteriochlorins were localized in mitochondria, lysosomes and endoplasmic reticulum as shown by organelle specific fluorescent probes. Cell death was via apoptosis as shown by cell morphology and nuclear condensation. Taken together, the results show the importance of appropriate peripheral groups about a photosensitizer for effective PDT applications.

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“…Dummin et al 8 did not observe changes of cell viability (measured with the method of trypan blue exclusion), cell division, and respiratory activity (even for the concentration of ZnPc of 50 µM), or changes of mitochondria ultrastructure. It is supposed that phthalocyanines bind to the inner membrane of mitochondria, especially to hydrophobic proteins of the oxidation chain, 14 although some authors claim that the outer mitochondrial membrane is their final target (due to low amount of cardiolipin). 28 Laser light irradiation with zinc phthalocyanine (Fig.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Depleted Glutathione Levels on the Photodynamic Action of Zinc Phthalocyanine in CHO K1 Cells

Krajewska

Bryszewska

Chapman

2003

Journal of Clinical Laser Medicine & Surgery

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Time-resolved microfluorometric study of the binding sites of lipophilic cationic pyrene probes in mitochondria of living HeLa cells

Cited by 25 publications

References 34 publications

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Effect of Adriamycin treatment on the lifetime of pyrene butyric acid in single living cells

Synthesis and evaluation of cationic bacteriochlorin amphiphiles with effective in vitro photodynamic activity against cancer cells at low nanomolar concentration

The Influence of Depleted Glutathione Levels on the Photodynamic Action of Zinc Phthalocyanine in CHO K1 Cells

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